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Construction of developmentally inspired periosteum-like tissue for bone regeneration

The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor re...

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Autores principales: Dai, Kai, Deng, Shunshu, Yu, Yuanman, Zhu, Fuwei, Wang, Jing, Liu, Changsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720863/
https://www.ncbi.nlm.nih.gov/pubmed/34975148
http://dx.doi.org/10.1038/s41413-021-00166-w
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author Dai, Kai
Deng, Shunshu
Yu, Yuanman
Zhu, Fuwei
Wang, Jing
Liu, Changsheng
author_facet Dai, Kai
Deng, Shunshu
Yu, Yuanman
Zhu, Fuwei
Wang, Jing
Liu, Changsheng
author_sort Dai, Kai
collection PubMed
description The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2 (BMP-2)-loaded scaffold to create periosteum-like tissue (PT) in vivo, mimicking the mesenchymal condensation during native long bone development. We found that BMP-2-induced endochondral ossification plays an indispensable role in the construction of PTs. Moreover, we confirmed that BMP-2-induced PTs exhibit a similar architecture to the periosteum and harbor abundant functional periosteum-like tissue-derived cells (PTDCs), blood vessels, and osteochondral progenitor cells. Interestingly, we found that the addition of chondroitin sulfate (CS), an essential component of the extracellular matrix (ECM), could further increase the abundance and enhance the function of recruited PTDCs from the PTs and finally increase the regenerative capacity of the PTs in autologous transplantation assays, even in old mice. This novel biomimetic strategy for generating PT through in vivo endochondral ossification deserves further clinical translation.
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spelling pubmed-87208632022-01-20 Construction of developmentally inspired periosteum-like tissue for bone regeneration Dai, Kai Deng, Shunshu Yu, Yuanman Zhu, Fuwei Wang, Jing Liu, Changsheng Bone Res Article The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2 (BMP-2)-loaded scaffold to create periosteum-like tissue (PT) in vivo, mimicking the mesenchymal condensation during native long bone development. We found that BMP-2-induced endochondral ossification plays an indispensable role in the construction of PTs. Moreover, we confirmed that BMP-2-induced PTs exhibit a similar architecture to the periosteum and harbor abundant functional periosteum-like tissue-derived cells (PTDCs), blood vessels, and osteochondral progenitor cells. Interestingly, we found that the addition of chondroitin sulfate (CS), an essential component of the extracellular matrix (ECM), could further increase the abundance and enhance the function of recruited PTDCs from the PTs and finally increase the regenerative capacity of the PTs in autologous transplantation assays, even in old mice. This novel biomimetic strategy for generating PT through in vivo endochondral ossification deserves further clinical translation. Nature Publishing Group UK 2022-01-03 /pmc/articles/PMC8720863/ /pubmed/34975148 http://dx.doi.org/10.1038/s41413-021-00166-w Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dai, Kai
Deng, Shunshu
Yu, Yuanman
Zhu, Fuwei
Wang, Jing
Liu, Changsheng
Construction of developmentally inspired periosteum-like tissue for bone regeneration
title Construction of developmentally inspired periosteum-like tissue for bone regeneration
title_full Construction of developmentally inspired periosteum-like tissue for bone regeneration
title_fullStr Construction of developmentally inspired periosteum-like tissue for bone regeneration
title_full_unstemmed Construction of developmentally inspired periosteum-like tissue for bone regeneration
title_short Construction of developmentally inspired periosteum-like tissue for bone regeneration
title_sort construction of developmentally inspired periosteum-like tissue for bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720863/
https://www.ncbi.nlm.nih.gov/pubmed/34975148
http://dx.doi.org/10.1038/s41413-021-00166-w
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