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Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae

Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them...

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Autores principales: Peng, Junbo, Wu, Linna, Zhang, Wei, Zhang, Qi, Xing, Qikai, Wang, Xuncheng, Li, Xinghong, Yan, Jiye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721227/
https://www.ncbi.nlm.nih.gov/pubmed/34987541
http://dx.doi.org/10.3389/fpls.2021.804696
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author Peng, Junbo
Wu, Linna
Zhang, Wei
Zhang, Qi
Xing, Qikai
Wang, Xuncheng
Li, Xinghong
Yan, Jiye
author_facet Peng, Junbo
Wu, Linna
Zhang, Wei
Zhang, Qi
Xing, Qikai
Wang, Xuncheng
Li, Xinghong
Yan, Jiye
author_sort Peng, Junbo
collection PubMed
description Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for LtCFEM2 and LtCFEM6, were significantly elevated during infection. In addition, almost all LtCFEM genes, apart from LtCFEM2, LtCFEM3, and LtCFEM6, were transcriptionally up-regulated at 35°C in contrast to that at 25°C and 30°C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in Nicotiana benthamiana leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in N. benthamiana. Moreover, two genes, LtCFEM7 and LtCFEM8, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen L. theobromae.
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spelling pubmed-87212272022-01-04 Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae Peng, Junbo Wu, Linna Zhang, Wei Zhang, Qi Xing, Qikai Wang, Xuncheng Li, Xinghong Yan, Jiye Front Plant Sci Plant Science Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for LtCFEM2 and LtCFEM6, were significantly elevated during infection. In addition, almost all LtCFEM genes, apart from LtCFEM2, LtCFEM3, and LtCFEM6, were transcriptionally up-regulated at 35°C in contrast to that at 25°C and 30°C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in Nicotiana benthamiana leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in N. benthamiana. Moreover, two genes, LtCFEM7 and LtCFEM8, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen L. theobromae. Frontiers Media S.A. 2021-12-20 /pmc/articles/PMC8721227/ /pubmed/34987541 http://dx.doi.org/10.3389/fpls.2021.804696 Text en Copyright © 2021 Peng, Wu, Zhang, Zhang, Xing, Wang, Li and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Peng, Junbo
Wu, Linna
Zhang, Wei
Zhang, Qi
Xing, Qikai
Wang, Xuncheng
Li, Xinghong
Yan, Jiye
Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title_full Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title_fullStr Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title_full_unstemmed Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title_short Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae
title_sort systemic identification and functional characterization of common in fungal extracellular membrane proteins in lasiodiplodia theobromae
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721227/
https://www.ncbi.nlm.nih.gov/pubmed/34987541
http://dx.doi.org/10.3389/fpls.2021.804696
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