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Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions
mRNA localization is an evolutionarily widespread phenomenon that can facilitate subcellular protein targeting. Extensive work has focused on mRNA targeting through ‘zip-codes’ within untranslated regions (UTRs), whereas much less is known about translation-dependent cues. Here, we examine mRNA loca...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722394/ https://www.ncbi.nlm.nih.gov/pubmed/34846063 http://dx.doi.org/10.1242/dev.200027 |
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author | Tocchini, Cristina Rohner, Michèle Guerard, Laurent Ray, Poulomi Von Stetina, Stephen E. Mango, Susan E. |
author_facet | Tocchini, Cristina Rohner, Michèle Guerard, Laurent Ray, Poulomi Von Stetina, Stephen E. Mango, Susan E. |
author_sort | Tocchini, Cristina |
collection | PubMed |
description | mRNA localization is an evolutionarily widespread phenomenon that can facilitate subcellular protein targeting. Extensive work has focused on mRNA targeting through ‘zip-codes’ within untranslated regions (UTRs), whereas much less is known about translation-dependent cues. Here, we examine mRNA localization in Caenorhabditis elegans embryonic epithelia. From an smFISH-based survey, we identified mRNAs associated with the cell membrane or cortex, and with apical junctions in a stage- and cell type-specific manner. Mutational analyses for one of these transcripts, dlg-1/discs large, revealed that it relied on a translation-dependent process and did not require its 5′ or 3′ UTRs. We suggest a model in which dlg-1 transcripts are co-translationally localized with the nascent protein: first the translating complex goes to the cell membrane using sequences located at the C-terminal/3′ end, and then apically using N-terminal/5′ sequences. These studies identify a translation-based process for mRNA localization within developing epithelia and determine the necessary cis-acting sequences for dlg-1 mRNA targeting. |
format | Online Article Text |
id | pubmed-8722394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-87223942022-01-26 Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions Tocchini, Cristina Rohner, Michèle Guerard, Laurent Ray, Poulomi Von Stetina, Stephen E. Mango, Susan E. Development Research Article mRNA localization is an evolutionarily widespread phenomenon that can facilitate subcellular protein targeting. Extensive work has focused on mRNA targeting through ‘zip-codes’ within untranslated regions (UTRs), whereas much less is known about translation-dependent cues. Here, we examine mRNA localization in Caenorhabditis elegans embryonic epithelia. From an smFISH-based survey, we identified mRNAs associated with the cell membrane or cortex, and with apical junctions in a stage- and cell type-specific manner. Mutational analyses for one of these transcripts, dlg-1/discs large, revealed that it relied on a translation-dependent process and did not require its 5′ or 3′ UTRs. We suggest a model in which dlg-1 transcripts are co-translationally localized with the nascent protein: first the translating complex goes to the cell membrane using sequences located at the C-terminal/3′ end, and then apically using N-terminal/5′ sequences. These studies identify a translation-based process for mRNA localization within developing epithelia and determine the necessary cis-acting sequences for dlg-1 mRNA targeting. The Company of Biologists Ltd 2021-12-16 /pmc/articles/PMC8722394/ /pubmed/34846063 http://dx.doi.org/10.1242/dev.200027 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Tocchini, Cristina Rohner, Michèle Guerard, Laurent Ray, Poulomi Von Stetina, Stephen E. Mango, Susan E. Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title | Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title_full | Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title_fullStr | Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title_full_unstemmed | Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title_short | Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions |
title_sort | translation-dependent mrna localization to caenorhabditis elegans adherens junctions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722394/ https://www.ncbi.nlm.nih.gov/pubmed/34846063 http://dx.doi.org/10.1242/dev.200027 |
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