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Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)

BACKGROUND AND STUDY AIMS: Previous studies have identified that colorectal cancer has different fucosylation levels compared to the normal colon. Ulex europaeus agglutinin-I (UEA-I), which specifically combines with α1-2 fucose glycan, is usually used to detect fucosylation levels. Therefore, we us...

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Autores principales: Wang, Weijun, Tian, Shuxin, Jiang, Xin, Pang, Suya, Shi, Huiying, Fan, Mengke, Wang, Zeyu, Jiang, Weiwei, Hu, Weiqian, Xiao, Xueyan, Lin, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722710/
https://www.ncbi.nlm.nih.gov/pubmed/34988023
http://dx.doi.org/10.3389/fonc.2021.792420
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author Wang, Weijun
Tian, Shuxin
Jiang, Xin
Pang, Suya
Shi, Huiying
Fan, Mengke
Wang, Zeyu
Jiang, Weiwei
Hu, Weiqian
Xiao, Xueyan
Lin, Rong
author_facet Wang, Weijun
Tian, Shuxin
Jiang, Xin
Pang, Suya
Shi, Huiying
Fan, Mengke
Wang, Zeyu
Jiang, Weiwei
Hu, Weiqian
Xiao, Xueyan
Lin, Rong
author_sort Wang, Weijun
collection PubMed
description BACKGROUND AND STUDY AIMS: Previous studies have identified that colorectal cancer has different fucosylation levels compared to the normal colon. Ulex europaeus agglutinin-I (UEA-I), which specifically combines with α1-2 fucose glycan, is usually used to detect fucosylation levels. Therefore, we used confocal laser endomicroscopy (CLE) to investigate fluorescently labeled UEA-Fluorescein isothiocyanate (FITC) for detecting colonic cancer. PATIENTS AND METHODS: We stained frozen mouse colon tissue sections of normal, adenoma, and adenocarcinoma species with UEA-FITC to detect fucosylation levels in different groups. White light endoscopy and endocytoscopy were first used to detect the lesions. The UEA-FITC was then stained in the mice and human colon tissues in vitro. The CLE was used to detect the UEA-FITC levels of the corresponding lesions, and videos were recorded for quantitation analysis. The diagnostic accuracy of UEA-FITC using CLE was evaluated in terms of sensitivity and specificity. RESULTS: The UEA expression level in colorectal cancer was lower than that in normal intestinal epithelium. The fluorescence intensity ratio of UEA-FITC in colorectal cancer was significantly lower than that in normal tissue detected by CLE in both mice and humans. The combination of UEA-FITC and CLE presented a good diagnostic accuracy with a sensitivity of 95.6% and a specificity of 97.7% for detecting colorectal cancer. The positive and negative predictive values were 91.6% and 95.6%, respectively. Overall, 95.6% of the sites were correctly classified by CLE. CONCLUSIONS: We developed a new imaging strategy to improve the diagnostic efficacy of CLE by using UEA-FITC.
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spelling pubmed-87227102022-01-04 Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video) Wang, Weijun Tian, Shuxin Jiang, Xin Pang, Suya Shi, Huiying Fan, Mengke Wang, Zeyu Jiang, Weiwei Hu, Weiqian Xiao, Xueyan Lin, Rong Front Oncol Oncology BACKGROUND AND STUDY AIMS: Previous studies have identified that colorectal cancer has different fucosylation levels compared to the normal colon. Ulex europaeus agglutinin-I (UEA-I), which specifically combines with α1-2 fucose glycan, is usually used to detect fucosylation levels. Therefore, we used confocal laser endomicroscopy (CLE) to investigate fluorescently labeled UEA-Fluorescein isothiocyanate (FITC) for detecting colonic cancer. PATIENTS AND METHODS: We stained frozen mouse colon tissue sections of normal, adenoma, and adenocarcinoma species with UEA-FITC to detect fucosylation levels in different groups. White light endoscopy and endocytoscopy were first used to detect the lesions. The UEA-FITC was then stained in the mice and human colon tissues in vitro. The CLE was used to detect the UEA-FITC levels of the corresponding lesions, and videos were recorded for quantitation analysis. The diagnostic accuracy of UEA-FITC using CLE was evaluated in terms of sensitivity and specificity. RESULTS: The UEA expression level in colorectal cancer was lower than that in normal intestinal epithelium. The fluorescence intensity ratio of UEA-FITC in colorectal cancer was significantly lower than that in normal tissue detected by CLE in both mice and humans. The combination of UEA-FITC and CLE presented a good diagnostic accuracy with a sensitivity of 95.6% and a specificity of 97.7% for detecting colorectal cancer. The positive and negative predictive values were 91.6% and 95.6%, respectively. Overall, 95.6% of the sites were correctly classified by CLE. CONCLUSIONS: We developed a new imaging strategy to improve the diagnostic efficacy of CLE by using UEA-FITC. Frontiers Media S.A. 2021-12-20 /pmc/articles/PMC8722710/ /pubmed/34988023 http://dx.doi.org/10.3389/fonc.2021.792420 Text en Copyright © 2021 Wang, Tian, Jiang, Pang, Shi, Fan, Wang, Jiang, Hu, Xiao and Lin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wang, Weijun
Tian, Shuxin
Jiang, Xin
Pang, Suya
Shi, Huiying
Fan, Mengke
Wang, Zeyu
Jiang, Weiwei
Hu, Weiqian
Xiao, Xueyan
Lin, Rong
Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title_full Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title_fullStr Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title_full_unstemmed Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title_short Molecular Imaging of Ulex Europaeus Agglutinin in Colorectal Cancer Using Confocal Laser Endomicroscopy (With Video)
title_sort molecular imaging of ulex europaeus agglutinin in colorectal cancer using confocal laser endomicroscopy (with video)
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8722710/
https://www.ncbi.nlm.nih.gov/pubmed/34988023
http://dx.doi.org/10.3389/fonc.2021.792420
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