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Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model
Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI(2))-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8723811/ https://www.ncbi.nlm.nih.gov/pubmed/34981388 http://dx.doi.org/10.1007/s11356-021-17781-z |
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author | Çavuşoğlu, Dilek Macar, Oksal Kalefetoğlu Macar, Tuğçe Çavuşoğlu, Kültiğin Yalçın, Emine |
author_facet | Çavuşoğlu, Dilek Macar, Oksal Kalefetoğlu Macar, Tuğçe Çavuşoğlu, Kültiğin Yalçın, Emine |
author_sort | Çavuşoğlu, Dilek |
collection | PubMed |
description | Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI(2))-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicity, and biochemical parameters as well as meristematic cell injuries in the roots were investigated. Ratios of catechin and caffeine in GTex were determined by HPLC analysis. Also, free radical scavenging activity of GTex was tested against superoxide and hydrogen peroxide radicals. As a result of HgCI(2) application, germination percentage, root elongation, weight gain, and mitotic index (MI) declined, while the frequency of micronucleus (MN), chromosomal abnormalities (CAs), and meristematic cell damages increased. HgCI(2) administration also led to a significant increase in malondialdehyde content, superoxide dismutase, and catalase activities which are signs of oxidative stress. On contrary, applications of GTex together with HgCI(2) reduced HgCI(2)-induced adverse effects in all parameters in a dose-dependent manner. Antioxidant components in GTex were listed as caffeine, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin according to their abundance. GTex exhibited a strong scavenging ability in the presence of superoxide and hydrogen peroxide radicals. The present study revealed the strong protective capacity of GTex against HgCI(2)-induced toxicity in A. cepa owing to its high antioxidant content with a multifaceted perspective. With this study, a reliable starting point was established for future studies investigating the more common and diverse use of GTex against toxic substances. |
format | Online Article Text |
id | pubmed-8723811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-87238112022-01-04 Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model Çavuşoğlu, Dilek Macar, Oksal Kalefetoğlu Macar, Tuğçe Çavuşoğlu, Kültiğin Yalçın, Emine Environ Sci Pollut Res Int Research Article Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI(2))-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicity, and biochemical parameters as well as meristematic cell injuries in the roots were investigated. Ratios of catechin and caffeine in GTex were determined by HPLC analysis. Also, free radical scavenging activity of GTex was tested against superoxide and hydrogen peroxide radicals. As a result of HgCI(2) application, germination percentage, root elongation, weight gain, and mitotic index (MI) declined, while the frequency of micronucleus (MN), chromosomal abnormalities (CAs), and meristematic cell damages increased. HgCI(2) administration also led to a significant increase in malondialdehyde content, superoxide dismutase, and catalase activities which are signs of oxidative stress. On contrary, applications of GTex together with HgCI(2) reduced HgCI(2)-induced adverse effects in all parameters in a dose-dependent manner. Antioxidant components in GTex were listed as caffeine, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin according to their abundance. GTex exhibited a strong scavenging ability in the presence of superoxide and hydrogen peroxide radicals. The present study revealed the strong protective capacity of GTex against HgCI(2)-induced toxicity in A. cepa owing to its high antioxidant content with a multifaceted perspective. With this study, a reliable starting point was established for future studies investigating the more common and diverse use of GTex against toxic substances. Springer Berlin Heidelberg 2022-01-04 2022 /pmc/articles/PMC8723811/ /pubmed/34981388 http://dx.doi.org/10.1007/s11356-021-17781-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021, corrected publication 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Article Çavuşoğlu, Dilek Macar, Oksal Kalefetoğlu Macar, Tuğçe Çavuşoğlu, Kültiğin Yalçın, Emine Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title | Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title_full | Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title_fullStr | Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title_full_unstemmed | Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title_short | Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model |
title_sort | mitigative effect of green tea extract against mercury(ii) chloride toxicity in allium cepa l. model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8723811/ https://www.ncbi.nlm.nih.gov/pubmed/34981388 http://dx.doi.org/10.1007/s11356-021-17781-z |
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