Cargando…
HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis
Hepatocellular carcinoma (HCC) is the most prevalent type of hepatic carcinoma. Long noncoding RNAs (lncRNAs) are considered crucial regulators of gene expression; however, their functions in HCC are not well understood. Thus, the present study is aimed at elucidating the functions of the lncRNA HOX...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8723882/ https://www.ncbi.nlm.nih.gov/pubmed/34988230 http://dx.doi.org/10.1155/2021/9289719 |
_version_ | 1784625816802426880 |
---|---|
author | Zeng, Cheng Ye, Shaojun Chen, Yu Zhang, Qu Luo, Yan Gai, Liang Luo, Bo |
author_facet | Zeng, Cheng Ye, Shaojun Chen, Yu Zhang, Qu Luo, Yan Gai, Liang Luo, Bo |
author_sort | Zeng, Cheng |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is the most prevalent type of hepatic carcinoma. Long noncoding RNAs (lncRNAs) are considered crucial regulators of gene expression; however, their functions in HCC are not well understood. Thus, the present study is aimed at elucidating the functions of the lncRNA HOXA-AS3 in HCC. The functions of the HOXA-AS3/miR-455-5p/programmed death-ligand 1 (PD-L1) axis were investigated in vitro via qRT-PCR and dual-luciferase reporter assays. The effect of HOXA-AS3 expression on tumor growth and metastasis was assessed using a mouse xenograft model. High HOXA-AS3 expression was observed in the HCC cell lines. Furthermore, overexpression of HOXA-AS3 in HCC cells enhanced proliferation, migration, and invasion, regulated the cell cycle, and retarded apoptosis. We also identified an miR-455-5p binding site in HOXA-AS3. By sponging miR-455-5p, HOXA-AS3 increased the expression of PD-L1. Additionally, both the inhibition of PD-L1 and overexpression of miR-455-5p reversed the effects on cell proliferation and invasion triggered by the overexpression of HOXA-AS3. In conclusion, HOXA-AS3 modulated the functions of HCC cells through the miR-455-5p/PD-L1 axis. Therefore, HOXA-AS3 may be a novel therapeutic target for HCC. |
format | Online Article Text |
id | pubmed-8723882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-87238822022-01-04 HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis Zeng, Cheng Ye, Shaojun Chen, Yu Zhang, Qu Luo, Yan Gai, Liang Luo, Bo J Immunol Res Research Article Hepatocellular carcinoma (HCC) is the most prevalent type of hepatic carcinoma. Long noncoding RNAs (lncRNAs) are considered crucial regulators of gene expression; however, their functions in HCC are not well understood. Thus, the present study is aimed at elucidating the functions of the lncRNA HOXA-AS3 in HCC. The functions of the HOXA-AS3/miR-455-5p/programmed death-ligand 1 (PD-L1) axis were investigated in vitro via qRT-PCR and dual-luciferase reporter assays. The effect of HOXA-AS3 expression on tumor growth and metastasis was assessed using a mouse xenograft model. High HOXA-AS3 expression was observed in the HCC cell lines. Furthermore, overexpression of HOXA-AS3 in HCC cells enhanced proliferation, migration, and invasion, regulated the cell cycle, and retarded apoptosis. We also identified an miR-455-5p binding site in HOXA-AS3. By sponging miR-455-5p, HOXA-AS3 increased the expression of PD-L1. Additionally, both the inhibition of PD-L1 and overexpression of miR-455-5p reversed the effects on cell proliferation and invasion triggered by the overexpression of HOXA-AS3. In conclusion, HOXA-AS3 modulated the functions of HCC cells through the miR-455-5p/PD-L1 axis. Therefore, HOXA-AS3 may be a novel therapeutic target for HCC. Hindawi 2021-12-27 /pmc/articles/PMC8723882/ /pubmed/34988230 http://dx.doi.org/10.1155/2021/9289719 Text en Copyright © 2021 Cheng Zeng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zeng, Cheng Ye, Shaojun Chen, Yu Zhang, Qu Luo, Yan Gai, Liang Luo, Bo HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title | HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title_full | HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title_fullStr | HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title_full_unstemmed | HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title_short | HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis |
title_sort | hoxa-as3 promotes proliferation and migration of hepatocellular carcinoma cells via the mir-455-5p/pd-l1 axis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8723882/ https://www.ncbi.nlm.nih.gov/pubmed/34988230 http://dx.doi.org/10.1155/2021/9289719 |
work_keys_str_mv | AT zengcheng hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT yeshaojun hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT chenyu hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT zhangqu hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT luoyan hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT gailiang hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis AT luobo hoxaas3promotesproliferationandmigrationofhepatocellularcarcinomacellsviathemir4555ppdl1axis |