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Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism

It is proposed that gluten- and casein-rich diets (GRD and CRD) can synergistically exacerbate dysbiosis as comorbidity in autism by worsening leaky gut that affects the brain through the gut–brain axis. In this study, 35 young male rats were divided into 7 groups, Group 1 serves as control; Group 2...

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Autores principales: Al Dera, Hussain, Alrafaei, Bahauddeen, AL Tamimi, Muneerah I., Alfawaz, Hanan A., Bhat, Ramesa Shafi, Soliman, Dina A., Abuaish, Sameera, El-Ansary, Afaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724359/
https://www.ncbi.nlm.nih.gov/pubmed/35070443
http://dx.doi.org/10.1515/tnsci-2020-0207
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author Al Dera, Hussain
Alrafaei, Bahauddeen
AL Tamimi, Muneerah I.
Alfawaz, Hanan A.
Bhat, Ramesa Shafi
Soliman, Dina A.
Abuaish, Sameera
El-Ansary, Afaf
author_facet Al Dera, Hussain
Alrafaei, Bahauddeen
AL Tamimi, Muneerah I.
Alfawaz, Hanan A.
Bhat, Ramesa Shafi
Soliman, Dina A.
Abuaish, Sameera
El-Ansary, Afaf
author_sort Al Dera, Hussain
collection PubMed
description It is proposed that gluten- and casein-rich diets (GRD and CRD) can synergistically exacerbate dysbiosis as comorbidity in autism by worsening leaky gut that affects the brain through the gut–brain axis. In this study, 35 young male rats were divided into 7 groups, Group 1 serves as control; Group 2, clindamycin (CL)-treated; and Group 3, propionic acid (PPA)-induced rodent model of autism. These three groups were fed standard diet until the end of the experiment. Groups 4–7 are rats treated similarly with CL and PPA, then fed on CRD or GRD until the end of the experiment. Serum zonulin, glutathione (GSH), lipid peroxides, and gut microbial composition were measured in the seven studied groups. Data demonstrate the significant increase in serum zonulin as marker of leaky gut in the CL-treated groups fed on CRD or GRD. Lipid peroxides were significantly higher in the serum of GRD-fed rats compared to CRD-fed or normal diet-fed rats. GSH was much lower in CL-treated groups fed on CRD or GRD compared to PPA-treated rats fed on both diets. Both diets differentially affected the diversity of the gut microbiota. This study demonstrates that CRD and GRD exacerbates leaky gut, according to serum zonulin, which was used as marker for increased gut permeability.
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spelling pubmed-87243592022-01-20 Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism Al Dera, Hussain Alrafaei, Bahauddeen AL Tamimi, Muneerah I. Alfawaz, Hanan A. Bhat, Ramesa Shafi Soliman, Dina A. Abuaish, Sameera El-Ansary, Afaf Transl Neurosci Research Article It is proposed that gluten- and casein-rich diets (GRD and CRD) can synergistically exacerbate dysbiosis as comorbidity in autism by worsening leaky gut that affects the brain through the gut–brain axis. In this study, 35 young male rats were divided into 7 groups, Group 1 serves as control; Group 2, clindamycin (CL)-treated; and Group 3, propionic acid (PPA)-induced rodent model of autism. These three groups were fed standard diet until the end of the experiment. Groups 4–7 are rats treated similarly with CL and PPA, then fed on CRD or GRD until the end of the experiment. Serum zonulin, glutathione (GSH), lipid peroxides, and gut microbial composition were measured in the seven studied groups. Data demonstrate the significant increase in serum zonulin as marker of leaky gut in the CL-treated groups fed on CRD or GRD. Lipid peroxides were significantly higher in the serum of GRD-fed rats compared to CRD-fed or normal diet-fed rats. GSH was much lower in CL-treated groups fed on CRD or GRD compared to PPA-treated rats fed on both diets. Both diets differentially affected the diversity of the gut microbiota. This study demonstrates that CRD and GRD exacerbates leaky gut, according to serum zonulin, which was used as marker for increased gut permeability. De Gruyter 2021-12-31 /pmc/articles/PMC8724359/ /pubmed/35070443 http://dx.doi.org/10.1515/tnsci-2020-0207 Text en © 2021 Hussain Al Dera et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Al Dera, Hussain
Alrafaei, Bahauddeen
AL Tamimi, Muneerah I.
Alfawaz, Hanan A.
Bhat, Ramesa Shafi
Soliman, Dina A.
Abuaish, Sameera
El-Ansary, Afaf
Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title_full Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title_fullStr Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title_full_unstemmed Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title_short Leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
title_sort leaky gut biomarkers in casein- and gluten-rich diet fed rat model of autism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724359/
https://www.ncbi.nlm.nih.gov/pubmed/35070443
http://dx.doi.org/10.1515/tnsci-2020-0207
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