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Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation

Chlorpyrifos (CPF), a widely used organophosphate pesticide, is reported to severely impair mammalian reproductive system. Pterostilbene (PTS), an effective free radical scavenger, is considered as beneficial for mammalian reproduction. However, the toxicity of CPF on oocyte maturation and whether P...

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Autores principales: Guo, Lili, Zhao, Yongda, Huan, Yanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724426/
https://www.ncbi.nlm.nih.gov/pubmed/34993205
http://dx.doi.org/10.3389/fcell.2021.803181
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author Guo, Lili
Zhao, Yongda
Huan, Yanjun
author_facet Guo, Lili
Zhao, Yongda
Huan, Yanjun
author_sort Guo, Lili
collection PubMed
description Chlorpyrifos (CPF), a widely used organophosphate pesticide, is reported to severely impair mammalian reproductive system. Pterostilbene (PTS), an effective free radical scavenger, is considered as beneficial for mammalian reproduction. However, the toxicity of CPF on oocyte maturation and whether PTS can eliminate the detrimental effect of CPF on oocytes remain unclear. Here, porcine oocytes were applied to investigate the potential effect and possible mechanism of CPF and PTS during oocyte maturation. This work demonstrated that CPF significantly delayed the meiotic progression and decreased the polar body extrusion by disturbing spindle assembly and chromosome alignment and causing DNA damage in oocytes (p < 0.05). And, CPF significantly impaired oocyte cytoplasmic maturation by inducing the high level of reactive oxygen species and decreasing glutathione content (p < 0.05). Moreover, CPF significantly triggered embryo apoptosis and reduced the blastocyst rate and cell number following parthenogenetic activation (p < 0.05). Whereas CPF-exposed oocytes were treated with PTS, these defects caused by CPF were obviously rescued, and oocyte maturation and subsequent embryonic development were also significantly ameliorated (p < 0.05). In conclusion, these results revealed that CPF exerted the toxic effect on porcine oocytes, while PTS effectively alleviated CPF-induced damage on oocytes. This work provides a potential strategy to protect oocyte maturation in mammalian species.
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spelling pubmed-87244262022-01-05 Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation Guo, Lili Zhao, Yongda Huan, Yanjun Front Cell Dev Biol Cell and Developmental Biology Chlorpyrifos (CPF), a widely used organophosphate pesticide, is reported to severely impair mammalian reproductive system. Pterostilbene (PTS), an effective free radical scavenger, is considered as beneficial for mammalian reproduction. However, the toxicity of CPF on oocyte maturation and whether PTS can eliminate the detrimental effect of CPF on oocytes remain unclear. Here, porcine oocytes were applied to investigate the potential effect and possible mechanism of CPF and PTS during oocyte maturation. This work demonstrated that CPF significantly delayed the meiotic progression and decreased the polar body extrusion by disturbing spindle assembly and chromosome alignment and causing DNA damage in oocytes (p < 0.05). And, CPF significantly impaired oocyte cytoplasmic maturation by inducing the high level of reactive oxygen species and decreasing glutathione content (p < 0.05). Moreover, CPF significantly triggered embryo apoptosis and reduced the blastocyst rate and cell number following parthenogenetic activation (p < 0.05). Whereas CPF-exposed oocytes were treated with PTS, these defects caused by CPF were obviously rescued, and oocyte maturation and subsequent embryonic development were also significantly ameliorated (p < 0.05). In conclusion, these results revealed that CPF exerted the toxic effect on porcine oocytes, while PTS effectively alleviated CPF-induced damage on oocytes. This work provides a potential strategy to protect oocyte maturation in mammalian species. Frontiers Media S.A. 2021-12-21 /pmc/articles/PMC8724426/ /pubmed/34993205 http://dx.doi.org/10.3389/fcell.2021.803181 Text en Copyright © 2021 Guo, Zhao and Huan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Guo, Lili
Zhao, Yongda
Huan, Yanjun
Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title_full Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title_fullStr Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title_full_unstemmed Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title_short Pterostilbene Alleviates Chlorpyrifos-Induced Damage During Porcine Oocyte Maturation
title_sort pterostilbene alleviates chlorpyrifos-induced damage during porcine oocyte maturation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724426/
https://www.ncbi.nlm.nih.gov/pubmed/34993205
http://dx.doi.org/10.3389/fcell.2021.803181
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