FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver

Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24(low) progenitors are less mature and have greater proliferative capacity than CD71/CD24(high). We present protocols for isolation of CD71/CD24(low) pr...

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Detalles Bibliográficos
Autores principales: Braun, Tatum W., Kuoch, Michael K., Khandros, Eugene, Li, Hojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724924/
https://www.ncbi.nlm.nih.gov/pubmed/35024628
http://dx.doi.org/10.1016/j.xpro.2021.101070
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author Braun, Tatum W.
Kuoch, Michael K.
Khandros, Eugene
Li, Hojun
author_facet Braun, Tatum W.
Kuoch, Michael K.
Khandros, Eugene
Li, Hojun
author_sort Braun, Tatum W.
collection PubMed
description Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24(low) progenitors are less mature and have greater proliferative capacity than CD71/CD24(high). We present protocols for isolation of CD71/CD24(low) progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24(low) progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).
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spelling pubmed-87249242022-01-11 FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver Braun, Tatum W. Kuoch, Michael K. Khandros, Eugene Li, Hojun STAR Protoc Protocol Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24(low) progenitors are less mature and have greater proliferative capacity than CD71/CD24(high). We present protocols for isolation of CD71/CD24(low) progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24(low) progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019). Elsevier 2021-12-29 /pmc/articles/PMC8724924/ /pubmed/35024628 http://dx.doi.org/10.1016/j.xpro.2021.101070 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Braun, Tatum W.
Kuoch, Michael K.
Khandros, Eugene
Li, Hojun
FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title_full FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title_fullStr FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title_full_unstemmed FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title_short FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
title_sort facs and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724924/
https://www.ncbi.nlm.nih.gov/pubmed/35024628
http://dx.doi.org/10.1016/j.xpro.2021.101070
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