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Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis

BACKGROUND: Neonates possess an immature and plastic immune system, which is a major cause of some diseases in newborns. Necrotizing enterocolitis (NEC) is a severe and devastating intestinal disease that typically affects premature infants. However, the development of intestinal immune cells in neo...

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Autores principales: Zhao, Xiuhao, Liang, Wenhua, Wang, Yonghui, Yi, Ruirong, Luo, Lingjie, Wang, Weifang, Sun, Nannan, Yu, Mingcheng, Xu, Weijue, Sheng, Qingfeng, Lu, Li, Pang, Jianfeng, Lv, Zhibao, Wang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725364/
https://www.ncbi.nlm.nih.gov/pubmed/34983626
http://dx.doi.org/10.1186/s13578-021-00739-6
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author Zhao, Xiuhao
Liang, Wenhua
Wang, Yonghui
Yi, Ruirong
Luo, Lingjie
Wang, Weifang
Sun, Nannan
Yu, Mingcheng
Xu, Weijue
Sheng, Qingfeng
Lu, Li
Pang, Jianfeng
Lv, Zhibao
Wang, Feng
author_facet Zhao, Xiuhao
Liang, Wenhua
Wang, Yonghui
Yi, Ruirong
Luo, Lingjie
Wang, Weifang
Sun, Nannan
Yu, Mingcheng
Xu, Weijue
Sheng, Qingfeng
Lu, Li
Pang, Jianfeng
Lv, Zhibao
Wang, Feng
author_sort Zhao, Xiuhao
collection PubMed
description BACKGROUND: Neonates possess an immature and plastic immune system, which is a major cause of some diseases in newborns. Necrotizing enterocolitis (NEC) is a severe and devastating intestinal disease that typically affects premature infants. However, the development of intestinal immune cells in neonates and their roles in the pathological process of NEC have not been elucidated. RESULTS: We examined the ontogeny of intestinal lamina propria lymphocytes in the early life of mice and found a high percentage of RORγt(+) cells (containing inflammatory Th17 and ILC3 populations) during the first week of life. Importantly, the proportion of RORγt(+) cells of intestinal lamina propria further increased in both NEC mice and patients tissue than the control. Furthermore, the application of GSK805, a specific antagonist of RORγt, inhibited IL-17A release and ameliorated NEC severity. CONCLUSIONS: Our data reveal the high proportion of RORγt(+) cells in newborn mice may directly contribute to the development of NEC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00739-6.
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spelling pubmed-87253642022-01-06 Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis Zhao, Xiuhao Liang, Wenhua Wang, Yonghui Yi, Ruirong Luo, Lingjie Wang, Weifang Sun, Nannan Yu, Mingcheng Xu, Weijue Sheng, Qingfeng Lu, Li Pang, Jianfeng Lv, Zhibao Wang, Feng Cell Biosci Research BACKGROUND: Neonates possess an immature and plastic immune system, which is a major cause of some diseases in newborns. Necrotizing enterocolitis (NEC) is a severe and devastating intestinal disease that typically affects premature infants. However, the development of intestinal immune cells in neonates and their roles in the pathological process of NEC have not been elucidated. RESULTS: We examined the ontogeny of intestinal lamina propria lymphocytes in the early life of mice and found a high percentage of RORγt(+) cells (containing inflammatory Th17 and ILC3 populations) during the first week of life. Importantly, the proportion of RORγt(+) cells of intestinal lamina propria further increased in both NEC mice and patients tissue than the control. Furthermore, the application of GSK805, a specific antagonist of RORγt, inhibited IL-17A release and ameliorated NEC severity. CONCLUSIONS: Our data reveal the high proportion of RORγt(+) cells in newborn mice may directly contribute to the development of NEC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-021-00739-6. BioMed Central 2022-01-04 /pmc/articles/PMC8725364/ /pubmed/34983626 http://dx.doi.org/10.1186/s13578-021-00739-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhao, Xiuhao
Liang, Wenhua
Wang, Yonghui
Yi, Ruirong
Luo, Lingjie
Wang, Weifang
Sun, Nannan
Yu, Mingcheng
Xu, Weijue
Sheng, Qingfeng
Lu, Li
Pang, Jianfeng
Lv, Zhibao
Wang, Feng
Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title_full Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title_fullStr Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title_full_unstemmed Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title_short Ontogeny of RORγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
title_sort ontogeny of rorγt(+) cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725364/
https://www.ncbi.nlm.nih.gov/pubmed/34983626
http://dx.doi.org/10.1186/s13578-021-00739-6
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