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Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella
Candida auris has globally emerged as a multidrug-resistant fungus linked to healthcare-associated outbreaks. There is still limited evidence on its virulence, pathogenicity determinants, and complex host–pathogen interactions. This study analyzes the in vivo fungal behaviour, immune response, and h...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725852/ https://www.ncbi.nlm.nih.gov/pubmed/34890523 http://dx.doi.org/10.1080/22221751.2021.2017756 |
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author | Garcia-Bustos, Victor Pemán, Javier Ruiz-Gaitán, Alba Cabañero-Navalon, Marta Dafne Cabanilles-Boronat, Ana Fernández-Calduch, María Marcilla-Barreda, Lucía Sigona-Giangreco, Ignacio A. Salavert, Miguel Tormo-Mas, María Ángeles Ruiz-Saurí, Amparo |
author_facet | Garcia-Bustos, Victor Pemán, Javier Ruiz-Gaitán, Alba Cabañero-Navalon, Marta Dafne Cabanilles-Boronat, Ana Fernández-Calduch, María Marcilla-Barreda, Lucía Sigona-Giangreco, Ignacio A. Salavert, Miguel Tormo-Mas, María Ángeles Ruiz-Saurí, Amparo |
author_sort | Garcia-Bustos, Victor |
collection | PubMed |
description | Candida auris has globally emerged as a multidrug-resistant fungus linked to healthcare-associated outbreaks. There is still limited evidence on its virulence, pathogenicity determinants, and complex host–pathogen interactions. This study analyzes the in vivo fungal behaviour, immune response, and host–pathogen interactions upon C. auris infection compared to C. albicans and C. parapsilosis in G. mellonella. This was performed by immunolabelling fungal structures and larval plasmatocytes and using a quantitative approach incorporating bioinformatic morphometric techniques into the study of microbial pathogenesis. C. auris presents a remarkably higher immunogenic activity than expected at its moderate degree of tissue invasion. It induces a greater inflammatory response than C. albicans and C. parapsilosis at the expense of plasmatocyte nodule formation, especially in non-aggregative strains. It specifically invades the larval respiratory system, in a pattern not previously observed in other Candida species, and presents inter-phenotypic tissue tropism differences. C. auris filaments in vivo less frequently than C. albicans or C. parapsilosis mostly through pseudohyphal growth. Filamentation might not be a major pathogenic determinant in C. auris, as less virulent aggregative phenotypes form pseudohyphae to a greater extent. C. auris has important both interspecific and intraspecific virulence and phenotype heterogeneity, with aggregative phenotypes of C. auris sharing characteristics with low pathogenic species such as C. parapsilosis. Our work suggests that C. auris owns an important morphogenetic plasticity that distinguishes it from other yeasts of the genus. Routine phenotypic identification of aggregative or non-aggregative phenotypes should be performed in the clinical setting as it may impact patient management. |
format | Online Article Text |
id | pubmed-8725852 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-87258522022-01-05 Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella Garcia-Bustos, Victor Pemán, Javier Ruiz-Gaitán, Alba Cabañero-Navalon, Marta Dafne Cabanilles-Boronat, Ana Fernández-Calduch, María Marcilla-Barreda, Lucía Sigona-Giangreco, Ignacio A. Salavert, Miguel Tormo-Mas, María Ángeles Ruiz-Saurí, Amparo Emerg Microbes Infect Research Article Candida auris has globally emerged as a multidrug-resistant fungus linked to healthcare-associated outbreaks. There is still limited evidence on its virulence, pathogenicity determinants, and complex host–pathogen interactions. This study analyzes the in vivo fungal behaviour, immune response, and host–pathogen interactions upon C. auris infection compared to C. albicans and C. parapsilosis in G. mellonella. This was performed by immunolabelling fungal structures and larval plasmatocytes and using a quantitative approach incorporating bioinformatic morphometric techniques into the study of microbial pathogenesis. C. auris presents a remarkably higher immunogenic activity than expected at its moderate degree of tissue invasion. It induces a greater inflammatory response than C. albicans and C. parapsilosis at the expense of plasmatocyte nodule formation, especially in non-aggregative strains. It specifically invades the larval respiratory system, in a pattern not previously observed in other Candida species, and presents inter-phenotypic tissue tropism differences. C. auris filaments in vivo less frequently than C. albicans or C. parapsilosis mostly through pseudohyphal growth. Filamentation might not be a major pathogenic determinant in C. auris, as less virulent aggregative phenotypes form pseudohyphae to a greater extent. C. auris has important both interspecific and intraspecific virulence and phenotype heterogeneity, with aggregative phenotypes of C. auris sharing characteristics with low pathogenic species such as C. parapsilosis. Our work suggests that C. auris owns an important morphogenetic plasticity that distinguishes it from other yeasts of the genus. Routine phenotypic identification of aggregative or non-aggregative phenotypes should be performed in the clinical setting as it may impact patient management. Taylor & Francis 2021-12-21 /pmc/articles/PMC8725852/ /pubmed/34890523 http://dx.doi.org/10.1080/22221751.2021.2017756 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Garcia-Bustos, Victor Pemán, Javier Ruiz-Gaitán, Alba Cabañero-Navalon, Marta Dafne Cabanilles-Boronat, Ana Fernández-Calduch, María Marcilla-Barreda, Lucía Sigona-Giangreco, Ignacio A. Salavert, Miguel Tormo-Mas, María Ángeles Ruiz-Saurí, Amparo Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title | Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title_full | Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title_fullStr | Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title_full_unstemmed | Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title_short | Host–pathogen interactions upon Candida auris infection: fungal behaviour and immune response in Galleria mellonella |
title_sort | host–pathogen interactions upon candida auris infection: fungal behaviour and immune response in galleria mellonella |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725852/ https://www.ncbi.nlm.nih.gov/pubmed/34890523 http://dx.doi.org/10.1080/22221751.2021.2017756 |
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