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Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE

PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were con...

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Autores principales: Altera, Annalisa, Barone, Virginia, Kondova, Ivanela, Langermans, Jan A. M., Gentile, Mariangela, Pin, Carmen, Nicoletti, Claudio, Bertelli, Eugenio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727310/
https://www.ncbi.nlm.nih.gov/pubmed/34967853
http://dx.doi.org/10.1167/iovs.62.15.32
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author Altera, Annalisa
Barone, Virginia
Kondova, Ivanela
Langermans, Jan A. M.
Gentile, Mariangela
Pin, Carmen
Nicoletti, Claudio
Bertelli, Eugenio
author_facet Altera, Annalisa
Barone, Virginia
Kondova, Ivanela
Langermans, Jan A. M.
Gentile, Mariangela
Pin, Carmen
Nicoletti, Claudio
Bertelli, Eugenio
author_sort Altera, Annalisa
collection PubMed
description PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were conducted on small fragments of retina sampled from different regions of the retina. RESULTS: RPE cells smooth endoplasmic reticulum shows a previously unknown arrangement characterized by an interlaced compartmental pattern (ICP). Electron tomograms and 3D-modelling demonstrated that the smooth endoplasmic reticulum with an ICP (ICPSER) consisted of four parallel, independent and interwoven networks of tubules arranged as interconnected coiled coils. Its architecture realized a compact labyrinthine structure of tightly packed tubules stabilized by intertubular filamentous tethers. On average, the ICPSER is present in about 14.6% of RPE cells. Although ICPSER was preferentially found in cells located in the peripheral and in the para/perifoveal retina, ICPSER cells significantly increased in number upon light exposure in the para/perifovea and in the fovea. CONCLUSIONS: An ICPSER is apparently a unique feature to primate RPE. Its rapid appearance in the area centralis of the retina upon light exposure suggests a function related to the foveate structure of primate retina or to the diurnal habits of animals that may require additional protection from photo-oxidation or enhanced requests of visual pigments regeneration.
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spelling pubmed-87273102022-01-14 Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE Altera, Annalisa Barone, Virginia Kondova, Ivanela Langermans, Jan A. M. Gentile, Mariangela Pin, Carmen Nicoletti, Claudio Bertelli, Eugenio Invest Ophthalmol Vis Sci Retinal Cell Biology PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were conducted on small fragments of retina sampled from different regions of the retina. RESULTS: RPE cells smooth endoplasmic reticulum shows a previously unknown arrangement characterized by an interlaced compartmental pattern (ICP). Electron tomograms and 3D-modelling demonstrated that the smooth endoplasmic reticulum with an ICP (ICPSER) consisted of four parallel, independent and interwoven networks of tubules arranged as interconnected coiled coils. Its architecture realized a compact labyrinthine structure of tightly packed tubules stabilized by intertubular filamentous tethers. On average, the ICPSER is present in about 14.6% of RPE cells. Although ICPSER was preferentially found in cells located in the peripheral and in the para/perifoveal retina, ICPSER cells significantly increased in number upon light exposure in the para/perifovea and in the fovea. CONCLUSIONS: An ICPSER is apparently a unique feature to primate RPE. Its rapid appearance in the area centralis of the retina upon light exposure suggests a function related to the foveate structure of primate retina or to the diurnal habits of animals that may require additional protection from photo-oxidation or enhanced requests of visual pigments regeneration. The Association for Research in Vision and Ophthalmology 2021-12-30 /pmc/articles/PMC8727310/ /pubmed/34967853 http://dx.doi.org/10.1167/iovs.62.15.32 Text en Copyright 2021 The Authors https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Retinal Cell Biology
Altera, Annalisa
Barone, Virginia
Kondova, Ivanela
Langermans, Jan A. M.
Gentile, Mariangela
Pin, Carmen
Nicoletti, Claudio
Bertelli, Eugenio
Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title_full Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title_fullStr Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title_full_unstemmed Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title_short Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
title_sort light-induced smooth endoplasmic reticulum rearrangement in a unique interlaced compartmental pattern in macaca mulatta rpe
topic Retinal Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727310/
https://www.ncbi.nlm.nih.gov/pubmed/34967853
http://dx.doi.org/10.1167/iovs.62.15.32
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