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Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE
PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were con...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Association for Research in Vision and Ophthalmology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727310/ https://www.ncbi.nlm.nih.gov/pubmed/34967853 http://dx.doi.org/10.1167/iovs.62.15.32 |
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author | Altera, Annalisa Barone, Virginia Kondova, Ivanela Langermans, Jan A. M. Gentile, Mariangela Pin, Carmen Nicoletti, Claudio Bertelli, Eugenio |
author_facet | Altera, Annalisa Barone, Virginia Kondova, Ivanela Langermans, Jan A. M. Gentile, Mariangela Pin, Carmen Nicoletti, Claudio Bertelli, Eugenio |
author_sort | Altera, Annalisa |
collection | PubMed |
description | PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were conducted on small fragments of retina sampled from different regions of the retina. RESULTS: RPE cells smooth endoplasmic reticulum shows a previously unknown arrangement characterized by an interlaced compartmental pattern (ICP). Electron tomograms and 3D-modelling demonstrated that the smooth endoplasmic reticulum with an ICP (ICPSER) consisted of four parallel, independent and interwoven networks of tubules arranged as interconnected coiled coils. Its architecture realized a compact labyrinthine structure of tightly packed tubules stabilized by intertubular filamentous tethers. On average, the ICPSER is present in about 14.6% of RPE cells. Although ICPSER was preferentially found in cells located in the peripheral and in the para/perifoveal retina, ICPSER cells significantly increased in number upon light exposure in the para/perifovea and in the fovea. CONCLUSIONS: An ICPSER is apparently a unique feature to primate RPE. Its rapid appearance in the area centralis of the retina upon light exposure suggests a function related to the foveate structure of primate retina or to the diurnal habits of animals that may require additional protection from photo-oxidation or enhanced requests of visual pigments regeneration. |
format | Online Article Text |
id | pubmed-8727310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-87273102022-01-14 Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE Altera, Annalisa Barone, Virginia Kondova, Ivanela Langermans, Jan A. M. Gentile, Mariangela Pin, Carmen Nicoletti, Claudio Bertelli, Eugenio Invest Ophthalmol Vis Sci Retinal Cell Biology PURPOSE: To investigate light-induced modifications of the smooth endoplasmic reticulum of the RPE in primates. METHODS: Eyes of three terminally anesthetized Rhesus monkeys were exposed to 5000 lux for 10 minutes or kept in the dark. Transmission electron microscopy and electron tomography were conducted on small fragments of retina sampled from different regions of the retina. RESULTS: RPE cells smooth endoplasmic reticulum shows a previously unknown arrangement characterized by an interlaced compartmental pattern (ICP). Electron tomograms and 3D-modelling demonstrated that the smooth endoplasmic reticulum with an ICP (ICPSER) consisted of four parallel, independent and interwoven networks of tubules arranged as interconnected coiled coils. Its architecture realized a compact labyrinthine structure of tightly packed tubules stabilized by intertubular filamentous tethers. On average, the ICPSER is present in about 14.6% of RPE cells. Although ICPSER was preferentially found in cells located in the peripheral and in the para/perifoveal retina, ICPSER cells significantly increased in number upon light exposure in the para/perifovea and in the fovea. CONCLUSIONS: An ICPSER is apparently a unique feature to primate RPE. Its rapid appearance in the area centralis of the retina upon light exposure suggests a function related to the foveate structure of primate retina or to the diurnal habits of animals that may require additional protection from photo-oxidation or enhanced requests of visual pigments regeneration. The Association for Research in Vision and Ophthalmology 2021-12-30 /pmc/articles/PMC8727310/ /pubmed/34967853 http://dx.doi.org/10.1167/iovs.62.15.32 Text en Copyright 2021 The Authors https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Retinal Cell Biology Altera, Annalisa Barone, Virginia Kondova, Ivanela Langermans, Jan A. M. Gentile, Mariangela Pin, Carmen Nicoletti, Claudio Bertelli, Eugenio Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title | Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title_full | Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title_fullStr | Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title_full_unstemmed | Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title_short | Light-Induced Smooth Endoplasmic Reticulum Rearrangement in a Unique Interlaced Compartmental Pattern in Macaca mulatta RPE |
title_sort | light-induced smooth endoplasmic reticulum rearrangement in a unique interlaced compartmental pattern in macaca mulatta rpe |
topic | Retinal Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727310/ https://www.ncbi.nlm.nih.gov/pubmed/34967853 http://dx.doi.org/10.1167/iovs.62.15.32 |
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