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Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis

Previous studies have shown that the miR-17-92 cluster is involved in the occurrence and development of bladder cancer. However, the role of serum miR-17-92 cluster in the diagnosis of bladder cancer has not been studied. In the present study, we evaluated the expression of miR-17-92 cluster members...

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Autores principales: Wang, Jingyao, Peng, Xiqi, Li, Rongkang, Liu, Kaihao, Zhang, Chunduo, Chen, Xuan, Huang, Guocheng, Zhao, Liwen, Chen, Zebo, Lai, Yongqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727362/
https://www.ncbi.nlm.nih.gov/pubmed/35004321
http://dx.doi.org/10.3389/fonc.2021.795837
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author Wang, Jingyao
Peng, Xiqi
Li, Rongkang
Liu, Kaihao
Zhang, Chunduo
Chen, Xuan
Huang, Guocheng
Zhao, Liwen
Chen, Zebo
Lai, Yongqing
author_facet Wang, Jingyao
Peng, Xiqi
Li, Rongkang
Liu, Kaihao
Zhang, Chunduo
Chen, Xuan
Huang, Guocheng
Zhao, Liwen
Chen, Zebo
Lai, Yongqing
author_sort Wang, Jingyao
collection PubMed
description Previous studies have shown that the miR-17-92 cluster is involved in the occurrence and development of bladder cancer. However, the role of serum miR-17-92 cluster in the diagnosis of bladder cancer has not been studied. In the present study, we evaluated the expression of miR-17-92 cluster members in bladder cancer tissues by analyzing 428 cases from TCGA database. Next, we collected the sera of 74 bladder cancer patients and 90 controls, and used qRT-PCR to detect the relative expression of the cluster. The results showed that the expression of the cluster members in the sera of patients were significantly higher than that of the controls, and they were positively correlated with the clinical stage and pathological grade of the patients. We evaluated their ability to diagnose bladder cancer using ROC, of which miR-92a-3p (AUC = 0.902), miR-17-5p (AUC = 0.845) and miR-20a-5p (AUC = 0.806) were the most prominent. Finally, we established a diagnostic model by logistic regression (AUC = 0.969). We further validated the results of the study using another dataset from the GEO database. Moreover, we evaluated the prognostic value of the cluster. The results revealed that miR-20a-5p was correlated with recurrence of bladder cancer. In summary, the present study validated the overexpression of serum miR-17-92 cluster in bladder cancer. The model composed of the three cluster members were confirmed to be a promising noninvasive biomarker for bladder cancer diagnosis.
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spelling pubmed-87273622022-01-06 Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis Wang, Jingyao Peng, Xiqi Li, Rongkang Liu, Kaihao Zhang, Chunduo Chen, Xuan Huang, Guocheng Zhao, Liwen Chen, Zebo Lai, Yongqing Front Oncol Oncology Previous studies have shown that the miR-17-92 cluster is involved in the occurrence and development of bladder cancer. However, the role of serum miR-17-92 cluster in the diagnosis of bladder cancer has not been studied. In the present study, we evaluated the expression of miR-17-92 cluster members in bladder cancer tissues by analyzing 428 cases from TCGA database. Next, we collected the sera of 74 bladder cancer patients and 90 controls, and used qRT-PCR to detect the relative expression of the cluster. The results showed that the expression of the cluster members in the sera of patients were significantly higher than that of the controls, and they were positively correlated with the clinical stage and pathological grade of the patients. We evaluated their ability to diagnose bladder cancer using ROC, of which miR-92a-3p (AUC = 0.902), miR-17-5p (AUC = 0.845) and miR-20a-5p (AUC = 0.806) were the most prominent. Finally, we established a diagnostic model by logistic regression (AUC = 0.969). We further validated the results of the study using another dataset from the GEO database. Moreover, we evaluated the prognostic value of the cluster. The results revealed that miR-20a-5p was correlated with recurrence of bladder cancer. In summary, the present study validated the overexpression of serum miR-17-92 cluster in bladder cancer. The model composed of the three cluster members were confirmed to be a promising noninvasive biomarker for bladder cancer diagnosis. Frontiers Media S.A. 2021-12-22 /pmc/articles/PMC8727362/ /pubmed/35004321 http://dx.doi.org/10.3389/fonc.2021.795837 Text en Copyright © 2021 Wang, Peng, Li, Liu, Zhang, Chen, Huang, Zhao, Chen and Lai https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wang, Jingyao
Peng, Xiqi
Li, Rongkang
Liu, Kaihao
Zhang, Chunduo
Chen, Xuan
Huang, Guocheng
Zhao, Liwen
Chen, Zebo
Lai, Yongqing
Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title_full Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title_fullStr Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title_full_unstemmed Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title_short Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis
title_sort evaluation of serum mir-17-92 cluster as noninvasive biomarkers for bladder cancer diagnosis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727362/
https://www.ncbi.nlm.nih.gov/pubmed/35004321
http://dx.doi.org/10.3389/fonc.2021.795837
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