Dual UMIs and Dual Barcodes With Minimal PCR Amplification Removes Artifacts and Acquires Accurate Antibody Repertoire

Antibody repertoire sequencing (Rep-seq) has been widely used to reveal repertoire dynamics and to interrogate antibodies of interest at single nucleotide-level resolution. However, polymerase chain reaction (PCR) amplification introduces extensive artifacts including chimeras and nucleotide errors,...

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Detalles Bibliográficos
Autores principales: Wang, Qilong, Zeng, Huikun, Zhu, Yan, Wang, Minhui, Zhang, Yanfang, Yang, Xiujia, Tang, Haipei, Li, Hongliang, Chen, Yuan, Ma, Cuiyu, Lan, Chunhong, Liu, Bin, Yang, Wei, Yu, Xueqing, Zhang, Zhenhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727365/
https://www.ncbi.nlm.nih.gov/pubmed/35003093
http://dx.doi.org/10.3389/fimmu.2021.778298

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