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Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment
Tumor innervation has recently been documented and characterized in various settings and tumor types. However, the role that nerves innervating tumors play in the pathogenesis of cancer has not been clarified. In this study, we searched for neural signaling from bulk RNA sequencing from The Cancer G...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728105/ https://www.ncbi.nlm.nih.gov/pubmed/35024574 http://dx.doi.org/10.1096/fba.2021-00076 |
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author | Dai, Hongyue Lou, Shan Zhang, Yanbo Thanawala, Monica Huang, Kai‐Chih Ji, Lexiang Carden, Sarah Liao, Tiffany Abbassi, Mandana Shu, Chengyi J. Lantermann, Alexandra Sadaghiani, Masoud Blom, Daniel Wagner, John Huang, Pearl |
author_facet | Dai, Hongyue Lou, Shan Zhang, Yanbo Thanawala, Monica Huang, Kai‐Chih Ji, Lexiang Carden, Sarah Liao, Tiffany Abbassi, Mandana Shu, Chengyi J. Lantermann, Alexandra Sadaghiani, Masoud Blom, Daniel Wagner, John Huang, Pearl |
author_sort | Dai, Hongyue |
collection | PubMed |
description | Tumor innervation has recently been documented and characterized in various settings and tumor types. However, the role that nerves innervating tumors play in the pathogenesis of cancer has not been clarified. In this study, we searched for neural signaling from bulk RNA sequencing from The Cancer Genome Atlas (TCGA) dataset and looked for patterns of interactions between different cell types within the tumor environment. Using a presynapse signature (PSS) as a probe, we showed that multiple stromal cell types crosstalk and/or contribute to neural signals. Based on the correlation and linear regression, we hypothesized that neural signals contribute to an immune‐suppressive tumor microenvironment (TME). To test this hypothesis, we performed in vitro dorsal root ganglion (DRG)/macrophage coculture experiments. Compared to the M2 macrophage monoculture, the DRG/M2 macrophage coculture prevented anti‐inflammatory M2 to pro‐inflammatory M1 polarization by LPS stimulation. Finally, a survey of different TCGA tumor types indicated that higher RNA neural signature is predictive of poor patient outcomes in multiple tumor types. |
format | Online Article Text |
id | pubmed-8728105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87281052022-01-11 Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment Dai, Hongyue Lou, Shan Zhang, Yanbo Thanawala, Monica Huang, Kai‐Chih Ji, Lexiang Carden, Sarah Liao, Tiffany Abbassi, Mandana Shu, Chengyi J. Lantermann, Alexandra Sadaghiani, Masoud Blom, Daniel Wagner, John Huang, Pearl FASEB Bioadv Research Articles Tumor innervation has recently been documented and characterized in various settings and tumor types. However, the role that nerves innervating tumors play in the pathogenesis of cancer has not been clarified. In this study, we searched for neural signaling from bulk RNA sequencing from The Cancer Genome Atlas (TCGA) dataset and looked for patterns of interactions between different cell types within the tumor environment. Using a presynapse signature (PSS) as a probe, we showed that multiple stromal cell types crosstalk and/or contribute to neural signals. Based on the correlation and linear regression, we hypothesized that neural signals contribute to an immune‐suppressive tumor microenvironment (TME). To test this hypothesis, we performed in vitro dorsal root ganglion (DRG)/macrophage coculture experiments. Compared to the M2 macrophage monoculture, the DRG/M2 macrophage coculture prevented anti‐inflammatory M2 to pro‐inflammatory M1 polarization by LPS stimulation. Finally, a survey of different TCGA tumor types indicated that higher RNA neural signature is predictive of poor patient outcomes in multiple tumor types. John Wiley and Sons Inc. 2021-10-04 /pmc/articles/PMC8728105/ /pubmed/35024574 http://dx.doi.org/10.1096/fba.2021-00076 Text en © 2021 Cygnal Therapeutics. FASEB BioAdvances published by Wiley Periodicals LLC on behalf of The Federation of American Societies for Experimental Biology https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Dai, Hongyue Lou, Shan Zhang, Yanbo Thanawala, Monica Huang, Kai‐Chih Ji, Lexiang Carden, Sarah Liao, Tiffany Abbassi, Mandana Shu, Chengyi J. Lantermann, Alexandra Sadaghiani, Masoud Blom, Daniel Wagner, John Huang, Pearl Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title | Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title_full | Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title_fullStr | Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title_full_unstemmed | Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title_short | Transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
title_sort | transcriptional neural‐like signaling contributes to an immune‐suppressive tumor microenvironment |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728105/ https://www.ncbi.nlm.nih.gov/pubmed/35024574 http://dx.doi.org/10.1096/fba.2021-00076 |
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