GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver

Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST-PFO tag enables analysis of the localization of choleste...

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Detalles Bibliográficos
Autores principales: Goicoechea, Leire, Arenas, Fabian, Castro, Fernanda, Nuñez, Susana, Torres, Sandra, Garcia-Ruiz, Carmen, Fernandez-Checa, José C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728529/
https://www.ncbi.nlm.nih.gov/pubmed/35024626
http://dx.doi.org/10.1016/j.xpro.2021.101068

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728529/
https://www.ncbi.nlm.nih.gov/pubmed/35024626
http://dx.doi.org/10.1016/j.xpro.2021.101068

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