Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel

Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining...

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Detalles Bibliográficos
Autores principales: Baracho, Gisele V., Kara, Nihan, Rigaud, Stephanie, Lo, Evelyn, Widmann, Stephanie J., Tyznik, Aaron J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728532/
https://www.ncbi.nlm.nih.gov/pubmed/35024627
http://dx.doi.org/10.1016/j.xpro.2021.101069
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author Baracho, Gisele V.
Kara, Nihan
Rigaud, Stephanie
Lo, Evelyn
Widmann, Stephanie J.
Tyznik, Aaron J.
author_facet Baracho, Gisele V.
Kara, Nihan
Rigaud, Stephanie
Lo, Evelyn
Widmann, Stephanie J.
Tyznik, Aaron J.
author_sort Baracho, Gisele V.
collection PubMed
description Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells.
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spelling pubmed-87285322022-01-11 Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel Baracho, Gisele V. Kara, Nihan Rigaud, Stephanie Lo, Evelyn Widmann, Stephanie J. Tyznik, Aaron J. STAR Protoc Protocol Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells. Elsevier 2021-12-29 /pmc/articles/PMC8728532/ /pubmed/35024627 http://dx.doi.org/10.1016/j.xpro.2021.101069 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Baracho, Gisele V.
Kara, Nihan
Rigaud, Stephanie
Lo, Evelyn
Widmann, Stephanie J.
Tyznik, Aaron J.
Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title_full Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title_fullStr Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title_full_unstemmed Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title_short Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel
title_sort functional phenotyping of circulating human cytotoxic t cells and nk cells using a 16-color flow cytometry panel
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728532/
https://www.ncbi.nlm.nih.gov/pubmed/35024627
http://dx.doi.org/10.1016/j.xpro.2021.101069
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