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Assembly of Multiple Full‐Size Genes or Genomic DNA Fragments on Human Artificial Chromosomes Using the Iterative Integration System

Human artificial chromosomes (HACs) are gene delivery vectors that have been used for decades for gene functional studies. HACs have several advantages over viral‐based gene transfer systems, including stable episomal maintenance in a single copy in the cell and the ability to carry up to megabase‐s...

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Detalles Bibliográficos
Autores principales:	Lee, Nicholas C. O., Petrov, Nikolai S., Larionov, Vladimir, Kouprina, Natalay
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730363/ https://www.ncbi.nlm.nih.gov/pubmed/34919348 http://dx.doi.org/10.1002/cpz1.316

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