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Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold

The males of many species of New World Phlebotomines produce volatile terpenoid chemicals, shown in Lutzomyia longipalpis s.l. to be sex/aggregation pheromones. Pheromone is produced by secretory cells which surround a cuticular reservoir which collects the pheromone and passes it through a cuticula...

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Autores principales: Tonelli, Gabriel B., Andrade-Filho, José D., Campos, Aldenise M., Margonari, Carina, Amaral, Amanda R., Volf, Petr, Shaw, Elisabeth J., Hamilton, James G. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730455/
https://www.ncbi.nlm.nih.gov/pubmed/34932549
http://dx.doi.org/10.1371/journal.pntd.0009733
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author Tonelli, Gabriel B.
Andrade-Filho, José D.
Campos, Aldenise M.
Margonari, Carina
Amaral, Amanda R.
Volf, Petr
Shaw, Elisabeth J.
Hamilton, James G. C.
author_facet Tonelli, Gabriel B.
Andrade-Filho, José D.
Campos, Aldenise M.
Margonari, Carina
Amaral, Amanda R.
Volf, Petr
Shaw, Elisabeth J.
Hamilton, James G. C.
author_sort Tonelli, Gabriel B.
collection PubMed
description The males of many species of New World Phlebotomines produce volatile terpenoid chemicals, shown in Lutzomyia longipalpis s.l. to be sex/aggregation pheromones. Pheromone is produced by secretory cells which surround a cuticular reservoir which collects the pheromone and passes it through a cuticular duct to the surface of the insect. The pheromone then passes through specialised cuticular structures on the abdominal surface prior to evaporation. The shape and distribution of the specialised structures are highly diverse and differ according to species. In this study we used SEM to examine the interior cuticular pheromone collection and transport structures of 3 members of the Lu. longipalpis s.l. species complex and Migonemyia migonei. We found a new structure which we have called the manifold which appears to be a substantial extension of the interior tergal cuticle connected in-line with the cuticular duct and reservoir. The manifold of the Campo Grande member of the complex is longer and wider than the Jacobina member whereas the manifold of the Sobral member was shorter than both other members of the complex. Overall, the secretory apparatus of the Sobral member was smaller than the other two. The manifold of M. migonei was very different to those found in Lu. longipalpis s.l. and was positioned in a pit-like structure within the tergal cuticle. The secretory reservoir was connected by a short duct to the manifold. Differences in the size and shape of the manifold may be related to the chemical structure of the pheromone and may have taxonomic value. Examination of the interior cuticle by SEM may help to locate the secretory apparatus of vector species where pheromonal activity has been inferred from behavioural studies but the external secretory structures or pheromones have not yet been found.
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spelling pubmed-87304552022-01-06 Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold Tonelli, Gabriel B. Andrade-Filho, José D. Campos, Aldenise M. Margonari, Carina Amaral, Amanda R. Volf, Petr Shaw, Elisabeth J. Hamilton, James G. C. PLoS Negl Trop Dis Research Article The males of many species of New World Phlebotomines produce volatile terpenoid chemicals, shown in Lutzomyia longipalpis s.l. to be sex/aggregation pheromones. Pheromone is produced by secretory cells which surround a cuticular reservoir which collects the pheromone and passes it through a cuticular duct to the surface of the insect. The pheromone then passes through specialised cuticular structures on the abdominal surface prior to evaporation. The shape and distribution of the specialised structures are highly diverse and differ according to species. In this study we used SEM to examine the interior cuticular pheromone collection and transport structures of 3 members of the Lu. longipalpis s.l. species complex and Migonemyia migonei. We found a new structure which we have called the manifold which appears to be a substantial extension of the interior tergal cuticle connected in-line with the cuticular duct and reservoir. The manifold of the Campo Grande member of the complex is longer and wider than the Jacobina member whereas the manifold of the Sobral member was shorter than both other members of the complex. Overall, the secretory apparatus of the Sobral member was smaller than the other two. The manifold of M. migonei was very different to those found in Lu. longipalpis s.l. and was positioned in a pit-like structure within the tergal cuticle. The secretory reservoir was connected by a short duct to the manifold. Differences in the size and shape of the manifold may be related to the chemical structure of the pheromone and may have taxonomic value. Examination of the interior cuticle by SEM may help to locate the secretory apparatus of vector species where pheromonal activity has been inferred from behavioural studies but the external secretory structures or pheromones have not yet been found. Public Library of Science 2021-12-21 /pmc/articles/PMC8730455/ /pubmed/34932549 http://dx.doi.org/10.1371/journal.pntd.0009733 Text en © 2021 Tonelli et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tonelli, Gabriel B.
Andrade-Filho, José D.
Campos, Aldenise M.
Margonari, Carina
Amaral, Amanda R.
Volf, Petr
Shaw, Elisabeth J.
Hamilton, James G. C.
Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title_full Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title_fullStr Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title_full_unstemmed Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title_short Examination of the interior of sand fly (Diptera: Psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: Discovering the manifold
title_sort examination of the interior of sand fly (diptera: psychodidae) abdomen reveals novel cuticular structures involved in pheromone release: discovering the manifold
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730455/
https://www.ncbi.nlm.nih.gov/pubmed/34932549
http://dx.doi.org/10.1371/journal.pntd.0009733
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