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Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study

BACKGROUND: The role of alterations in gut microbiota composition (termed dysbiosis) has been implicated in the pathobiology of depressive symptoms; however, evidence remains limited. This cross-sectional pilot study is aimed at exploring whether depressive symptom scores changed during neoadjuvant...

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Autores principales: Gonzalez-Mercado, Velda J., Lim, Jean, Saligan, Leorey N., Perez, Nicole, Rodriguez, Carmen, Bernabe, Raul, Ozorio, Samia, Pedro, Elsa, Sepehri, Farrah, Aouizerat, Brad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8731300/
https://www.ncbi.nlm.nih.gov/pubmed/35003805
http://dx.doi.org/10.1155/2021/7967552
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author Gonzalez-Mercado, Velda J.
Lim, Jean
Saligan, Leorey N.
Perez, Nicole
Rodriguez, Carmen
Bernabe, Raul
Ozorio, Samia
Pedro, Elsa
Sepehri, Farrah
Aouizerat, Brad
author_facet Gonzalez-Mercado, Velda J.
Lim, Jean
Saligan, Leorey N.
Perez, Nicole
Rodriguez, Carmen
Bernabe, Raul
Ozorio, Samia
Pedro, Elsa
Sepehri, Farrah
Aouizerat, Brad
author_sort Gonzalez-Mercado, Velda J.
collection PubMed
description BACKGROUND: The role of alterations in gut microbiota composition (termed dysbiosis) has been implicated in the pathobiology of depressive symptoms; however, evidence remains limited. This cross-sectional pilot study is aimed at exploring whether depressive symptom scores changed during neoadjuvant chemotherapy and radiation therapy to treat rectal cancer, and if gut microbial taxa abundances and predicted functional pathways correlate with depressive symptoms at the end of chemotherapy and radiation therapy. METHODS: 40 newly diagnosed rectal cancer patients (ages 28-81; 23 males) were assessed for depressive symptoms using the Hamilton Rating Scale for Depression (HAM-D) and provided stool samples for 16S rRNA sequencing. Gut microbiome data were analyzed using QIIME2, and correlations and regression analyses were performed in R. RESULTS: Participants had significantly higher depressive symptoms at the end as compared to before CRT. The relative abundances of Gemella, Bacillales Family XI, Actinomyces, Streptococcus, Lactococcus, Weissella, and Leuconostocaceae were positively correlated (Spearman's rho = 0.42 to 0.32), while Coprobacter, Intestinibacter, Intestimonas, Lachnospiraceae, Phascolarctobacterium, Ruminiclostridium, Ruminococcaceae (UCG-005 and uncultured), Tyzzerella, and Parasutterella (Spearman's rho = −0.43 to − 0.31) were negatively correlated with HAM-D scores. Of the 14 predicted MetaCyc pathways that correlated with depressive symptom scores at the end of CRT, 11 (79%) were associated with biosynthetic pathways. CONCLUSIONS: Significant bacterial taxa and predicted functional pathways correlated with depressive symptoms at the end of chemotherapy and radiation therapy for rectal cancer which warrants further examination and replication of our findings.
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spelling pubmed-87313002022-01-06 Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study Gonzalez-Mercado, Velda J. Lim, Jean Saligan, Leorey N. Perez, Nicole Rodriguez, Carmen Bernabe, Raul Ozorio, Samia Pedro, Elsa Sepehri, Farrah Aouizerat, Brad Depress Res Treat Research Article BACKGROUND: The role of alterations in gut microbiota composition (termed dysbiosis) has been implicated in the pathobiology of depressive symptoms; however, evidence remains limited. This cross-sectional pilot study is aimed at exploring whether depressive symptom scores changed during neoadjuvant chemotherapy and radiation therapy to treat rectal cancer, and if gut microbial taxa abundances and predicted functional pathways correlate with depressive symptoms at the end of chemotherapy and radiation therapy. METHODS: 40 newly diagnosed rectal cancer patients (ages 28-81; 23 males) were assessed for depressive symptoms using the Hamilton Rating Scale for Depression (HAM-D) and provided stool samples for 16S rRNA sequencing. Gut microbiome data were analyzed using QIIME2, and correlations and regression analyses were performed in R. RESULTS: Participants had significantly higher depressive symptoms at the end as compared to before CRT. The relative abundances of Gemella, Bacillales Family XI, Actinomyces, Streptococcus, Lactococcus, Weissella, and Leuconostocaceae were positively correlated (Spearman's rho = 0.42 to 0.32), while Coprobacter, Intestinibacter, Intestimonas, Lachnospiraceae, Phascolarctobacterium, Ruminiclostridium, Ruminococcaceae (UCG-005 and uncultured), Tyzzerella, and Parasutterella (Spearman's rho = −0.43 to − 0.31) were negatively correlated with HAM-D scores. Of the 14 predicted MetaCyc pathways that correlated with depressive symptom scores at the end of CRT, 11 (79%) were associated with biosynthetic pathways. CONCLUSIONS: Significant bacterial taxa and predicted functional pathways correlated with depressive symptoms at the end of chemotherapy and radiation therapy for rectal cancer which warrants further examination and replication of our findings. Hindawi 2021-12-29 /pmc/articles/PMC8731300/ /pubmed/35003805 http://dx.doi.org/10.1155/2021/7967552 Text en Copyright © 2021 Velda J. Gonzalez-Mercado et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gonzalez-Mercado, Velda J.
Lim, Jean
Saligan, Leorey N.
Perez, Nicole
Rodriguez, Carmen
Bernabe, Raul
Ozorio, Samia
Pedro, Elsa
Sepehri, Farrah
Aouizerat, Brad
Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title_full Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title_fullStr Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title_full_unstemmed Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title_short Gut Microbiota and Depressive Symptoms at the End of CRT for Rectal Cancer: A Cross-Sectional Pilot Study
title_sort gut microbiota and depressive symptoms at the end of crt for rectal cancer: a cross-sectional pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8731300/
https://www.ncbi.nlm.nih.gov/pubmed/35003805
http://dx.doi.org/10.1155/2021/7967552
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