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Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity

Palladium (Pd) is a widely used metal and extremely important biomaterial for the reconstruction of occlusions during dental restorations. However, metallic biomaterials can cause serious allergic reactions, such as Pd-related oral mucositis seen in dentistry. Metal allergy is categorized as a type...

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Autores principales: Ito, Koyu, Kanaseki, Takayuki, Tokita, Serina, Torigoe, Toshihiko, Hirasawa, Noriyasu, Ogasawara, Kouetsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732370/
https://www.ncbi.nlm.nih.gov/pubmed/35003059
http://dx.doi.org/10.3389/fimmu.2021.736936
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author Ito, Koyu
Kanaseki, Takayuki
Tokita, Serina
Torigoe, Toshihiko
Hirasawa, Noriyasu
Ogasawara, Kouetsu
author_facet Ito, Koyu
Kanaseki, Takayuki
Tokita, Serina
Torigoe, Toshihiko
Hirasawa, Noriyasu
Ogasawara, Kouetsu
author_sort Ito, Koyu
collection PubMed
description Palladium (Pd) is a widely used metal and extremely important biomaterial for the reconstruction of occlusions during dental restorations. However, metallic biomaterials can cause serious allergic reactions, such as Pd-related oral mucositis seen in dentistry. Metal allergy is categorized as a type IV allergy and we demonstrated that CD8 T cells play an important role in Pd allergy previously. As TCR of CD8 T cells recognizes MHC class I/peptide complex, the antigen specificity to this complex seems to be generated during Pd allergy. However, it remains unknown if Pd affects the MHC class I/peptide complex. In this study, we investigated the behavior of the MHC class I/peptide complex in response to Pd treatment. We found that PdCl(2) treatment altered peptide presentation on MHC class I and that co-culture with Pd-treated DC2.4 cells induced activation of Pd-responsive TCR-expressing T cell line. Furthermore, PdCl(2) treatment induced temporal MHC class I internalization and inhibition of membrane movement suppressed Pd-induced T cell-mediated antigenicity. These data suggest that Pd-induced MHC class I internalization is critical for generation of antigenicity through a mechanism including differential peptide loading on MHC class I, which results in Pd allergy.
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spelling pubmed-87323702022-01-07 Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity Ito, Koyu Kanaseki, Takayuki Tokita, Serina Torigoe, Toshihiko Hirasawa, Noriyasu Ogasawara, Kouetsu Front Immunol Immunology Palladium (Pd) is a widely used metal and extremely important biomaterial for the reconstruction of occlusions during dental restorations. However, metallic biomaterials can cause serious allergic reactions, such as Pd-related oral mucositis seen in dentistry. Metal allergy is categorized as a type IV allergy and we demonstrated that CD8 T cells play an important role in Pd allergy previously. As TCR of CD8 T cells recognizes MHC class I/peptide complex, the antigen specificity to this complex seems to be generated during Pd allergy. However, it remains unknown if Pd affects the MHC class I/peptide complex. In this study, we investigated the behavior of the MHC class I/peptide complex in response to Pd treatment. We found that PdCl(2) treatment altered peptide presentation on MHC class I and that co-culture with Pd-treated DC2.4 cells induced activation of Pd-responsive TCR-expressing T cell line. Furthermore, PdCl(2) treatment induced temporal MHC class I internalization and inhibition of membrane movement suppressed Pd-induced T cell-mediated antigenicity. These data suggest that Pd-induced MHC class I internalization is critical for generation of antigenicity through a mechanism including differential peptide loading on MHC class I, which results in Pd allergy. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8732370/ /pubmed/35003059 http://dx.doi.org/10.3389/fimmu.2021.736936 Text en Copyright © 2021 Ito, Kanaseki, Tokita, Torigoe, Hirasawa and Ogasawara https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ito, Koyu
Kanaseki, Takayuki
Tokita, Serina
Torigoe, Toshihiko
Hirasawa, Noriyasu
Ogasawara, Kouetsu
Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title_full Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title_fullStr Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title_full_unstemmed Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title_short Palladium-Induced Temporal Internalization of MHC Class I Contributes to T Cell-Mediated Antigenicity
title_sort palladium-induced temporal internalization of mhc class i contributes to t cell-mediated antigenicity
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732370/
https://www.ncbi.nlm.nih.gov/pubmed/35003059
http://dx.doi.org/10.3389/fimmu.2021.736936
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