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Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT
Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s synd...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732922/ https://www.ncbi.nlm.nih.gov/pubmed/34590290 http://dx.doi.org/10.1007/s13577-021-00621-0 |
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author | Asakawa, Takeyoshi Yamada, Atsushi Kugino, Masumi Hasegawa, Tomokazu Yoshimura, Kentaro Sasa, Kiyohito Kinoshita, Mitsuhiro Nitta, Masakazu Nagata, Karin Sugiyama, Tomomi Kamijo, Ryutaro Funatsu, Takahiro |
author_facet | Asakawa, Takeyoshi Yamada, Atsushi Kugino, Masumi Hasegawa, Tomokazu Yoshimura, Kentaro Sasa, Kiyohito Kinoshita, Mitsuhiro Nitta, Masakazu Nagata, Karin Sugiyama, Tomomi Kamijo, Ryutaro Funatsu, Takahiro |
author_sort | Asakawa, Takeyoshi |
collection | PubMed |
description | Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13577-021-00621-0. |
format | Online Article Text |
id | pubmed-8732922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-87329222022-01-18 Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT Asakawa, Takeyoshi Yamada, Atsushi Kugino, Masumi Hasegawa, Tomokazu Yoshimura, Kentaro Sasa, Kiyohito Kinoshita, Mitsuhiro Nitta, Masakazu Nagata, Karin Sugiyama, Tomomi Kamijo, Ryutaro Funatsu, Takahiro Hum Cell Cell Line Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13577-021-00621-0. Springer Singapore 2021-09-29 2022 /pmc/articles/PMC8732922/ /pubmed/34590290 http://dx.doi.org/10.1007/s13577-021-00621-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Cell Line Asakawa, Takeyoshi Yamada, Atsushi Kugino, Masumi Hasegawa, Tomokazu Yoshimura, Kentaro Sasa, Kiyohito Kinoshita, Mitsuhiro Nitta, Masakazu Nagata, Karin Sugiyama, Tomomi Kamijo, Ryutaro Funatsu, Takahiro Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title | Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title_full | Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title_fullStr | Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title_full_unstemmed | Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title_short | Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT |
title_sort | establishment of down’s syndrome periodontal ligament cells by transfection with sv40t-ag and htert |
topic | Cell Line |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732922/ https://www.ncbi.nlm.nih.gov/pubmed/34590290 http://dx.doi.org/10.1007/s13577-021-00621-0 |
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