CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples

The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared the semi-quantitative, cassette-based ReaScan CXCL13 assay with the quantitative recomBead CXCL13 assay using a collection of 209 cerebrospinal fluid samples. The categ...

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Autores principales: Haglund, Sofie, Lager, Malin, Gyllemark, Paula, Andersson, Gärda, Ekelund, Oskar, Sundqvist, Martin, Henningsson, Anna J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732935/
https://www.ncbi.nlm.nih.gov/pubmed/34626256
http://dx.doi.org/10.1007/s10096-021-04350-y
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author Haglund, Sofie
Lager, Malin
Gyllemark, Paula
Andersson, Gärda
Ekelund, Oskar
Sundqvist, Martin
Henningsson, Anna J.
author_facet Haglund, Sofie
Lager, Malin
Gyllemark, Paula
Andersson, Gärda
Ekelund, Oskar
Sundqvist, Martin
Henningsson, Anna J.
author_sort Haglund, Sofie
collection PubMed
description The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared the semi-quantitative, cassette-based ReaScan CXCL13 assay with the quantitative recomBead CXCL13 assay using a collection of 209 cerebrospinal fluid samples. The categorical agreement between results interpreted as negative, grey zone, and positive by the two methods was 87%. The diagnostic sensitivity was higher using the recomBead assay, whereas specificity was higher using ReaScan. Few manual steps, and a short turn-around time with no batching of samples makes the ReaScan CXCL13 assay an attractive complement to serology in the diagnostics of LNB. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-021-04350-y.
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spelling pubmed-87329352022-01-18 CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples Haglund, Sofie Lager, Malin Gyllemark, Paula Andersson, Gärda Ekelund, Oskar Sundqvist, Martin Henningsson, Anna J. Eur J Clin Microbiol Infect Dis Brief Report The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared the semi-quantitative, cassette-based ReaScan CXCL13 assay with the quantitative recomBead CXCL13 assay using a collection of 209 cerebrospinal fluid samples. The categorical agreement between results interpreted as negative, grey zone, and positive by the two methods was 87%. The diagnostic sensitivity was higher using the recomBead assay, whereas specificity was higher using ReaScan. Few manual steps, and a short turn-around time with no batching of samples makes the ReaScan CXCL13 assay an attractive complement to serology in the diagnostics of LNB. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-021-04350-y. Springer Berlin Heidelberg 2021-10-09 2022 /pmc/articles/PMC8732935/ /pubmed/34626256 http://dx.doi.org/10.1007/s10096-021-04350-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Haglund, Sofie
Lager, Malin
Gyllemark, Paula
Andersson, Gärda
Ekelund, Oskar
Sundqvist, Martin
Henningsson, Anna J.
CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title_full CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title_fullStr CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title_full_unstemmed CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title_short CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
title_sort cxcl13 in laboratory diagnosis of lyme neuroborreliosis—the performance of the recombead and reascan cxcl13 assays in human cerebrospinal fluid samples
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8732935/
https://www.ncbi.nlm.nih.gov/pubmed/34626256
http://dx.doi.org/10.1007/s10096-021-04350-y
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