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An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733245/ https://www.ncbi.nlm.nih.gov/pubmed/35004629 http://dx.doi.org/10.3389/fchem.2021.813219 |
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author | Zhu, Jianyu Dou, Leina Shao, Shibei Kou, Jiaqian Yu, Xuezhi Wen, Kai Wang, Zhanhui Yu, Wenbo |
author_facet | Zhu, Jianyu Dou, Leina Shao, Shibei Kou, Jiaqian Yu, Xuezhi Wen, Kai Wang, Zhanhui Yu, Wenbo |
author_sort | Zhu, Jianyu |
collection | PubMed |
description | Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapid diagnosis of mushroom poisoning. In this study, we established a highly sensitive and automated magnetic bead (MB)-based chemiluminescence immunoassay (CLIA) for the early, rapid diagnosis of mushroom poisoning. The limits of detection (LODs) for phallotoxins were 0.010 ng/ml in human serum and 0.009 ng/ml in human urine. Recoveries ranged from 81.6 to 95.6% with a coefficient of variation <12.9%. Analysis of Amanita phalloides samples by the automated MB-based CLIA was in accordance with that of HPLC-MS/MS. The advantages the MB-based CLIA, high sensitivity, repeatability, and stability, were due to the use of MBs as immune carriers, chemiluminescence as a detection signal, and an integrated device to automate the whole process. Therefore, the proposed automated MB-based CLIA is a promising option for the early and rapid clinical diagnosis of mushroom poisoning. |
format | Online Article Text |
id | pubmed-8733245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87332452022-01-07 An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning Zhu, Jianyu Dou, Leina Shao, Shibei Kou, Jiaqian Yu, Xuezhi Wen, Kai Wang, Zhanhui Yu, Wenbo Front Chem Chemistry Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapid diagnosis of mushroom poisoning. In this study, we established a highly sensitive and automated magnetic bead (MB)-based chemiluminescence immunoassay (CLIA) for the early, rapid diagnosis of mushroom poisoning. The limits of detection (LODs) for phallotoxins were 0.010 ng/ml in human serum and 0.009 ng/ml in human urine. Recoveries ranged from 81.6 to 95.6% with a coefficient of variation <12.9%. Analysis of Amanita phalloides samples by the automated MB-based CLIA was in accordance with that of HPLC-MS/MS. The advantages the MB-based CLIA, high sensitivity, repeatability, and stability, were due to the use of MBs as immune carriers, chemiluminescence as a detection signal, and an integrated device to automate the whole process. Therefore, the proposed automated MB-based CLIA is a promising option for the early and rapid clinical diagnosis of mushroom poisoning. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733245/ /pubmed/35004629 http://dx.doi.org/10.3389/fchem.2021.813219 Text en Copyright © 2021 Zhu, Dou, Shao, Kou, Yu, Wen, Wang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Zhu, Jianyu Dou, Leina Shao, Shibei Kou, Jiaqian Yu, Xuezhi Wen, Kai Wang, Zhanhui Yu, Wenbo An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title | An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title_full | An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title_fullStr | An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title_full_unstemmed | An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title_short | An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning |
title_sort | automated and highly sensitive chemiluminescence immunoassay for diagnosing mushroom poisoning |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733245/ https://www.ncbi.nlm.nih.gov/pubmed/35004629 http://dx.doi.org/10.3389/fchem.2021.813219 |
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