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An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning

Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapi...

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Autores principales: Zhu, Jianyu, Dou, Leina, Shao, Shibei, Kou, Jiaqian, Yu, Xuezhi, Wen, Kai, Wang, Zhanhui, Yu, Wenbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733245/
https://www.ncbi.nlm.nih.gov/pubmed/35004629
http://dx.doi.org/10.3389/fchem.2021.813219
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author Zhu, Jianyu
Dou, Leina
Shao, Shibei
Kou, Jiaqian
Yu, Xuezhi
Wen, Kai
Wang, Zhanhui
Yu, Wenbo
author_facet Zhu, Jianyu
Dou, Leina
Shao, Shibei
Kou, Jiaqian
Yu, Xuezhi
Wen, Kai
Wang, Zhanhui
Yu, Wenbo
author_sort Zhu, Jianyu
collection PubMed
description Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapid diagnosis of mushroom poisoning. In this study, we established a highly sensitive and automated magnetic bead (MB)-based chemiluminescence immunoassay (CLIA) for the early, rapid diagnosis of mushroom poisoning. The limits of detection (LODs) for phallotoxins were 0.010 ng/ml in human serum and 0.009 ng/ml in human urine. Recoveries ranged from 81.6 to 95.6% with a coefficient of variation <12.9%. Analysis of Amanita phalloides samples by the automated MB-based CLIA was in accordance with that of HPLC-MS/MS. The advantages the MB-based CLIA, high sensitivity, repeatability, and stability, were due to the use of MBs as immune carriers, chemiluminescence as a detection signal, and an integrated device to automate the whole process. Therefore, the proposed automated MB-based CLIA is a promising option for the early and rapid clinical diagnosis of mushroom poisoning.
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spelling pubmed-87332452022-01-07 An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning Zhu, Jianyu Dou, Leina Shao, Shibei Kou, Jiaqian Yu, Xuezhi Wen, Kai Wang, Zhanhui Yu, Wenbo Front Chem Chemistry Mushrooms containing Amanita peptide toxins are the major cause of mushroom poisoning, and lead to approximately 90% of deaths. Phallotoxins are the fastest toxin causing poisoning among Amanita peptide toxins. Thus, it is imperative to construct a highly sensitive quantification method for the rapid diagnosis of mushroom poisoning. In this study, we established a highly sensitive and automated magnetic bead (MB)-based chemiluminescence immunoassay (CLIA) for the early, rapid diagnosis of mushroom poisoning. The limits of detection (LODs) for phallotoxins were 0.010 ng/ml in human serum and 0.009 ng/ml in human urine. Recoveries ranged from 81.6 to 95.6% with a coefficient of variation <12.9%. Analysis of Amanita phalloides samples by the automated MB-based CLIA was in accordance with that of HPLC-MS/MS. The advantages the MB-based CLIA, high sensitivity, repeatability, and stability, were due to the use of MBs as immune carriers, chemiluminescence as a detection signal, and an integrated device to automate the whole process. Therefore, the proposed automated MB-based CLIA is a promising option for the early and rapid clinical diagnosis of mushroom poisoning. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733245/ /pubmed/35004629 http://dx.doi.org/10.3389/fchem.2021.813219 Text en Copyright © 2021 Zhu, Dou, Shao, Kou, Yu, Wen, Wang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Zhu, Jianyu
Dou, Leina
Shao, Shibei
Kou, Jiaqian
Yu, Xuezhi
Wen, Kai
Wang, Zhanhui
Yu, Wenbo
An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title_full An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title_fullStr An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title_full_unstemmed An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title_short An Automated and Highly Sensitive Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning
title_sort automated and highly sensitive chemiluminescence immunoassay for diagnosing mushroom poisoning
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733245/
https://www.ncbi.nlm.nih.gov/pubmed/35004629
http://dx.doi.org/10.3389/fchem.2021.813219
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