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The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana

Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and...

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Autores principales: Krahmer, Johanna, Hindle, Matthew, Perby, Laura K., Mogensen, Helle K., Nielsen, Tom H., Halliday, Karen J., van Ooijen, Gerben, Le Bihan, Thierry, Millar, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733343/
https://www.ncbi.nlm.nih.gov/pubmed/34740825
http://dx.doi.org/10.1016/j.mcpro.2021.100172
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author Krahmer, Johanna
Hindle, Matthew
Perby, Laura K.
Mogensen, Helle K.
Nielsen, Tom H.
Halliday, Karen J.
van Ooijen, Gerben
Le Bihan, Thierry
Millar, Andrew J.
author_facet Krahmer, Johanna
Hindle, Matthew
Perby, Laura K.
Mogensen, Helle K.
Nielsen, Tom H.
Halliday, Karen J.
van Ooijen, Gerben
Le Bihan, Thierry
Millar, Andrew J.
author_sort Krahmer, Johanna
collection PubMed
description Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and phosphoproteomic time series from Arabidopsis thaliana plants under constant light conditions, estimating that just 0.4% of quantified proteins but a much larger proportion of quantified phospho-sites were rhythmic. Approximately half of the rhythmic phospho-sites were most phosphorylated at subjective dawn, a pattern we term the “phospho-dawn.” Members of the SnRK/CDPK family of protein kinases are candidate regulators. A CCA1-overexpressing line that disables the clock gene circuit lacked most circadian protein phosphorylation. However, the few phospho-sites that fluctuated despite CCA1-overexpression still tended to peak in abundance close to subjective dawn, suggesting that the canonical clock mechanism is necessary for most but perhaps not all protein phosphorylation rhythms. To test the potential functional relevance of our datasets, we conducted phosphomimetic experiments using the bifunctional enzyme fructose-6-phosphate-2-kinase/phosphatase (F2KP), as an example. The rhythmic phosphorylation of diverse protein targets is controlled by the clock gene circuit, implicating posttranslational mechanisms in the transmission of circadian timing information in plants.
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spelling pubmed-87333432022-01-11 The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana Krahmer, Johanna Hindle, Matthew Perby, Laura K. Mogensen, Helle K. Nielsen, Tom H. Halliday, Karen J. van Ooijen, Gerben Le Bihan, Thierry Millar, Andrew J. Mol Cell Proteomics Research Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and phosphoproteomic time series from Arabidopsis thaliana plants under constant light conditions, estimating that just 0.4% of quantified proteins but a much larger proportion of quantified phospho-sites were rhythmic. Approximately half of the rhythmic phospho-sites were most phosphorylated at subjective dawn, a pattern we term the “phospho-dawn.” Members of the SnRK/CDPK family of protein kinases are candidate regulators. A CCA1-overexpressing line that disables the clock gene circuit lacked most circadian protein phosphorylation. However, the few phospho-sites that fluctuated despite CCA1-overexpression still tended to peak in abundance close to subjective dawn, suggesting that the canonical clock mechanism is necessary for most but perhaps not all protein phosphorylation rhythms. To test the potential functional relevance of our datasets, we conducted phosphomimetic experiments using the bifunctional enzyme fructose-6-phosphate-2-kinase/phosphatase (F2KP), as an example. The rhythmic phosphorylation of diverse protein targets is controlled by the clock gene circuit, implicating posttranslational mechanisms in the transmission of circadian timing information in plants. American Society for Biochemistry and Molecular Biology 2021-11-03 /pmc/articles/PMC8733343/ /pubmed/34740825 http://dx.doi.org/10.1016/j.mcpro.2021.100172 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research
Krahmer, Johanna
Hindle, Matthew
Perby, Laura K.
Mogensen, Helle K.
Nielsen, Tom H.
Halliday, Karen J.
van Ooijen, Gerben
Le Bihan, Thierry
Millar, Andrew J.
The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title_full The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title_fullStr The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title_full_unstemmed The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title_short The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana
title_sort circadian clock gene circuit controls protein and phosphoprotein rhythms in arabidopsis thaliana
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733343/
https://www.ncbi.nlm.nih.gov/pubmed/34740825
http://dx.doi.org/10.1016/j.mcpro.2021.100172
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