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AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.

Artemisinin is an important drug for resistance against malaria. Artemisinin is derived from the glandular trichome of leaves, stems, or buds of the Chinese traditional herb Artemisia annua. Increasing the trichome density may enhance the artemisinin content of A. annua. It has been proven that cycl...

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Autores principales: Dong, Boran, Wang, Xingxing, Jiang, Rui, Fang, Shiyuan, Li, Jinxing, Li, Qing, Lv, Zong you, Chen, Wan sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733389/
https://www.ncbi.nlm.nih.gov/pubmed/35003194
http://dx.doi.org/10.3389/fpls.2021.808283
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author Dong, Boran
Wang, Xingxing
Jiang, Rui
Fang, Shiyuan
Li, Jinxing
Li, Qing
Lv, Zong you
Chen, Wan sheng
author_facet Dong, Boran
Wang, Xingxing
Jiang, Rui
Fang, Shiyuan
Li, Jinxing
Li, Qing
Lv, Zong you
Chen, Wan sheng
author_sort Dong, Boran
collection PubMed
description Artemisinin is an important drug for resistance against malaria. Artemisinin is derived from the glandular trichome of leaves, stems, or buds of the Chinese traditional herb Artemisia annua. Increasing the trichome density may enhance the artemisinin content of A. annua. It has been proven that cyclins are involved in the development of trichomes in tomato, Arabidopsis, and tobacco, but it is unclear whether the cyclins in A. annua influence trichome development. In this study, we showed that AaCycTL may regulate trichome development and affect the content of artemisinin. We cloned AaCycTL and found that it has the same expression files as the artemisinin biosynthesis pathway gene. We overexpressed AaCycTL in Arabidopsis, and the results indicated that AaCycTL changed the wax coverage on the surface of Arabidopsis leaves. The trichome density decreased as well. Using yeast two-hybrid and BiFC assays, we show that AaCycTL can interact with AaTAR1. Moreover, we overexpressed AaCycTL in A. annua and found that the expression of AaCycTL was increased to 82–195%. Changes in wax coverage on the surface of transgenic A. annua leaves or stems were found as well. We identified the expression of the artemisinin biosynthesis pathway genes ADS, CYP71AV1, and ALDH1 has decreased to 88–98%, 76–97%, and 82–97% in the AaCycTL-overexpressing A. annua lines, respectively. Furthermore, we found reduced the content of artemisinin. In agreement, overexpression of AaCycTL in A. annua or Arabidopsis may alter waxy loading, change the initiation of trichomes and downregulate trichome density. Altogether, AaCycTL mediates trichome development in A. annua and thus may serve to regulate trichome density and be used for artemisinin biosynthesis.
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spelling pubmed-87333892022-01-07 AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L. Dong, Boran Wang, Xingxing Jiang, Rui Fang, Shiyuan Li, Jinxing Li, Qing Lv, Zong you Chen, Wan sheng Front Plant Sci Plant Science Artemisinin is an important drug for resistance against malaria. Artemisinin is derived from the glandular trichome of leaves, stems, or buds of the Chinese traditional herb Artemisia annua. Increasing the trichome density may enhance the artemisinin content of A. annua. It has been proven that cyclins are involved in the development of trichomes in tomato, Arabidopsis, and tobacco, but it is unclear whether the cyclins in A. annua influence trichome development. In this study, we showed that AaCycTL may regulate trichome development and affect the content of artemisinin. We cloned AaCycTL and found that it has the same expression files as the artemisinin biosynthesis pathway gene. We overexpressed AaCycTL in Arabidopsis, and the results indicated that AaCycTL changed the wax coverage on the surface of Arabidopsis leaves. The trichome density decreased as well. Using yeast two-hybrid and BiFC assays, we show that AaCycTL can interact with AaTAR1. Moreover, we overexpressed AaCycTL in A. annua and found that the expression of AaCycTL was increased to 82–195%. Changes in wax coverage on the surface of transgenic A. annua leaves or stems were found as well. We identified the expression of the artemisinin biosynthesis pathway genes ADS, CYP71AV1, and ALDH1 has decreased to 88–98%, 76–97%, and 82–97% in the AaCycTL-overexpressing A. annua lines, respectively. Furthermore, we found reduced the content of artemisinin. In agreement, overexpression of AaCycTL in A. annua or Arabidopsis may alter waxy loading, change the initiation of trichomes and downregulate trichome density. Altogether, AaCycTL mediates trichome development in A. annua and thus may serve to regulate trichome density and be used for artemisinin biosynthesis. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733389/ /pubmed/35003194 http://dx.doi.org/10.3389/fpls.2021.808283 Text en Copyright © 2021 Dong, Wang, Jiang, Fang, Li, Li, Lv and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Dong, Boran
Wang, Xingxing
Jiang, Rui
Fang, Shiyuan
Li, Jinxing
Li, Qing
Lv, Zong you
Chen, Wan sheng
AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title_full AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title_fullStr AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title_full_unstemmed AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title_short AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.
title_sort aacyctl regulates cuticle and trichome development in arabidopsis and artemisia annua l.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733389/
https://www.ncbi.nlm.nih.gov/pubmed/35003194
http://dx.doi.org/10.3389/fpls.2021.808283
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