Cargando…

Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re

Panax notoginseng saponins (PNS) have been used to treat cardiovascular diseases for hundreds of years in China. Lysozyme can bind to exogenous compounds and promote their activity. Nevertheless, knowledge of whether there is a synergistic role between lysozyme and PNS is far from sufficient. In thi...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Yun, Cui, Lijian, Yang, Hongchao, Chen, Ning, Guo, Huishan, Gan, Xiaoruo, Wang, Rong, Shi, Weiye, Wu, Yu, Zhang, Yan, Lv, Pin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733556/
https://www.ncbi.nlm.nih.gov/pubmed/35004822
http://dx.doi.org/10.3389/fnut.2021.795888
_version_ 1784627822191443968
author Huang, Yun
Cui, Lijian
Yang, Hongchao
Chen, Ning
Guo, Huishan
Gan, Xiaoruo
Wang, Rong
Shi, Weiye
Wu, Yu
Zhang, Yan
Lv, Pin
author_facet Huang, Yun
Cui, Lijian
Yang, Hongchao
Chen, Ning
Guo, Huishan
Gan, Xiaoruo
Wang, Rong
Shi, Weiye
Wu, Yu
Zhang, Yan
Lv, Pin
author_sort Huang, Yun
collection PubMed
description Panax notoginseng saponins (PNS) have been used to treat cardiovascular diseases for hundreds of years in China. Lysozyme can bind to exogenous compounds and promote their activity. Nevertheless, knowledge of whether there is a synergistic role between lysozyme and PNS is far from sufficient. In this study, we show that the mixture of PNS and lysozyme synergistically inhibited platelet derived growth factor BB (PDGF-BB)-induced vascular smooth muscle cell (VSMC) viability, and in the five main components of PNS, GS-Re, but not GS-Rb1, NG-R1, GS-Rg1, or GS-Rd, reduced VSMC viability by combined application with lysozyme. Next, the supramolecular complexes formed by GS-Re and lysozyme were detected by mass spectrometry, and the binding ability increased with the concentration ratio of GS-Re to lysozyme from 4:1 to 12:1. In the supramolecular complexes, the relative contents of α-helix of lysozyme were increased, which was beneficial for stabilizing the structure of lysozyme. The 12:1 mixture of GS-Re and lysozyme (12.8 μmol/L GS-Re+1.067 μmol/L lysozyme) repressed PDGF-BB-induced VSMC viability, proliferation, and migration, which were associated with the upregulated differentiated markers and downregulated dedifferentiated markers. Finally, in CaCl(2)-induced rodent abdominal aortic aneurysm (AAA) models, we found that the 12:1 mixture of GS-Re and lysozyme slowed down AAA progression and reversed phenotype transformation of VSMCs. Thus, Gs-Re combined with a small amount of lysozyme may provide a novel therapeutic strategy for vascular remodeling-associated cardiovascular diseases.
format Online
Article
Text
id pubmed-8733556
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87335562022-01-07 Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re Huang, Yun Cui, Lijian Yang, Hongchao Chen, Ning Guo, Huishan Gan, Xiaoruo Wang, Rong Shi, Weiye Wu, Yu Zhang, Yan Lv, Pin Front Nutr Nutrition Panax notoginseng saponins (PNS) have been used to treat cardiovascular diseases for hundreds of years in China. Lysozyme can bind to exogenous compounds and promote their activity. Nevertheless, knowledge of whether there is a synergistic role between lysozyme and PNS is far from sufficient. In this study, we show that the mixture of PNS and lysozyme synergistically inhibited platelet derived growth factor BB (PDGF-BB)-induced vascular smooth muscle cell (VSMC) viability, and in the five main components of PNS, GS-Re, but not GS-Rb1, NG-R1, GS-Rg1, or GS-Rd, reduced VSMC viability by combined application with lysozyme. Next, the supramolecular complexes formed by GS-Re and lysozyme were detected by mass spectrometry, and the binding ability increased with the concentration ratio of GS-Re to lysozyme from 4:1 to 12:1. In the supramolecular complexes, the relative contents of α-helix of lysozyme were increased, which was beneficial for stabilizing the structure of lysozyme. The 12:1 mixture of GS-Re and lysozyme (12.8 μmol/L GS-Re+1.067 μmol/L lysozyme) repressed PDGF-BB-induced VSMC viability, proliferation, and migration, which were associated with the upregulated differentiated markers and downregulated dedifferentiated markers. Finally, in CaCl(2)-induced rodent abdominal aortic aneurysm (AAA) models, we found that the 12:1 mixture of GS-Re and lysozyme slowed down AAA progression and reversed phenotype transformation of VSMCs. Thus, Gs-Re combined with a small amount of lysozyme may provide a novel therapeutic strategy for vascular remodeling-associated cardiovascular diseases. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733556/ /pubmed/35004822 http://dx.doi.org/10.3389/fnut.2021.795888 Text en Copyright © 2021 Huang, Cui, Yang, Chen, Guo, Gan, Wang, Shi, Wu, Zhang and Lv. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Huang, Yun
Cui, Lijian
Yang, Hongchao
Chen, Ning
Guo, Huishan
Gan, Xiaoruo
Wang, Rong
Shi, Weiye
Wu, Yu
Zhang, Yan
Lv, Pin
Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title_full Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title_fullStr Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title_full_unstemmed Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title_short Lysozyme Improves the Inhibitory Effects of Panax notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells by Binding to Ginsenoside Re
title_sort lysozyme improves the inhibitory effects of panax notoginseng saponins on phenotype transformation of vascular smooth muscle cells by binding to ginsenoside re
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733556/
https://www.ncbi.nlm.nih.gov/pubmed/35004822
http://dx.doi.org/10.3389/fnut.2021.795888
work_keys_str_mv AT huangyun lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT cuilijian lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT yanghongchao lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT chenning lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT guohuishan lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT ganxiaoruo lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT wangrong lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT shiweiye lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT wuyu lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT zhangyan lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere
AT lvpin lysozymeimprovestheinhibitoryeffectsofpanaxnotoginsengsaponinsonphenotypetransformationofvascularsmoothmusclecellsbybindingtoginsenosidere