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Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response

Drought is one of the most important environmental constraints affecting plant growth and development and ultimately leads to yield loss. Uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) are believed to play key roles in coping with environmental stresses. In rice, it is estimated tha...

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Autores principales: Liu, Qian, Dong, Guang-rui, Ma, Yu-qing, Zhao, Shu-man, Liu, Xi, Li, Xing-kun, Li, Yan-jie, Hou, Bing-kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733621/
https://www.ncbi.nlm.nih.gov/pubmed/35003178
http://dx.doi.org/10.3389/fpls.2021.790195
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author Liu, Qian
Dong, Guang-rui
Ma, Yu-qing
Zhao, Shu-man
Liu, Xi
Li, Xing-kun
Li, Yan-jie
Hou, Bing-kai
author_facet Liu, Qian
Dong, Guang-rui
Ma, Yu-qing
Zhao, Shu-man
Liu, Xi
Li, Xing-kun
Li, Yan-jie
Hou, Bing-kai
author_sort Liu, Qian
collection PubMed
description Drought is one of the most important environmental constraints affecting plant growth and development and ultimately leads to yield loss. Uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) are believed to play key roles in coping with environmental stresses. In rice, it is estimated that there are more than 200 UGT genes. However, most of them have not been identified as their physiological significance. In this study, we reported the characterization of a putative glycosyltransferase gene UGT85E1 in rice. UGT85E1 gene is significantly upregulated by drought stress and abscisic acid (ABA) treatment. The overexpression of UGT85E1 led to an enhanced tolerance in transgenic rice plants to drought stress, while the ugt85e1 mutants of rice showed a more sensitive phenotype to drought stress. Further studies indicated that UGT85E1 overexpression induced ABA accumulation, stomatal closure, enhanced reactive oxygen species (ROS) scavenging capacity, increased proline and sugar contents, and upregulated expression of stress-related genes under drought stress conditions. Moreover, when UGT85E1 was ectopically overexpressed in Arabidopsis, the transgenic plants showed increased tolerance to drought as well as in rice. Our findings suggest that UGT85E1 plays an important role in mediating plant response to drought and oxidative stresses. This work may provide a promising candidate gene for cultivating drought-tolerant crops both in dicots and monocots.
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spelling pubmed-87336212022-01-07 Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response Liu, Qian Dong, Guang-rui Ma, Yu-qing Zhao, Shu-man Liu, Xi Li, Xing-kun Li, Yan-jie Hou, Bing-kai Front Plant Sci Plant Science Drought is one of the most important environmental constraints affecting plant growth and development and ultimately leads to yield loss. Uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) are believed to play key roles in coping with environmental stresses. In rice, it is estimated that there are more than 200 UGT genes. However, most of them have not been identified as their physiological significance. In this study, we reported the characterization of a putative glycosyltransferase gene UGT85E1 in rice. UGT85E1 gene is significantly upregulated by drought stress and abscisic acid (ABA) treatment. The overexpression of UGT85E1 led to an enhanced tolerance in transgenic rice plants to drought stress, while the ugt85e1 mutants of rice showed a more sensitive phenotype to drought stress. Further studies indicated that UGT85E1 overexpression induced ABA accumulation, stomatal closure, enhanced reactive oxygen species (ROS) scavenging capacity, increased proline and sugar contents, and upregulated expression of stress-related genes under drought stress conditions. Moreover, when UGT85E1 was ectopically overexpressed in Arabidopsis, the transgenic plants showed increased tolerance to drought as well as in rice. Our findings suggest that UGT85E1 plays an important role in mediating plant response to drought and oxidative stresses. This work may provide a promising candidate gene for cultivating drought-tolerant crops both in dicots and monocots. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733621/ /pubmed/35003178 http://dx.doi.org/10.3389/fpls.2021.790195 Text en Copyright © 2021 Liu, Dong, Ma, Zhao, Liu, Li, Li and Hou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Qian
Dong, Guang-rui
Ma, Yu-qing
Zhao, Shu-man
Liu, Xi
Li, Xing-kun
Li, Yan-jie
Hou, Bing-kai
Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title_full Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title_fullStr Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title_full_unstemmed Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title_short Rice Glycosyltransferase Gene UGT85E1 Is Involved in Drought Stress Tolerance Through Enhancing Abscisic Acid Response
title_sort rice glycosyltransferase gene ugt85e1 is involved in drought stress tolerance through enhancing abscisic acid response
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733621/
https://www.ncbi.nlm.nih.gov/pubmed/35003178
http://dx.doi.org/10.3389/fpls.2021.790195
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