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Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species

As two cultivated widely allotetraploid cotton species, although Gossypium hirsutum and Gossypium barbadense evolved from the same ancestor, they differ in fiber quality; the molecular mechanism of that difference should be deeply studied. Here, we performed RNA-seq of fiber samples from four G. hir...

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Autores principales: Mei, Huan, Qi, Bowen, Han, Zegang, Zhao, Ting, Guo, Menglan, Han, Jin, Zhang, Juncheng, Guan, Xueying, Hu, Yan, Zhang, Tianzhen, Fang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733733/
https://www.ncbi.nlm.nih.gov/pubmed/35003198
http://dx.doi.org/10.3389/fpls.2021.819679
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author Mei, Huan
Qi, Bowen
Han, Zegang
Zhao, Ting
Guo, Menglan
Han, Jin
Zhang, Juncheng
Guan, Xueying
Hu, Yan
Zhang, Tianzhen
Fang, Lei
author_facet Mei, Huan
Qi, Bowen
Han, Zegang
Zhao, Ting
Guo, Menglan
Han, Jin
Zhang, Juncheng
Guan, Xueying
Hu, Yan
Zhang, Tianzhen
Fang, Lei
author_sort Mei, Huan
collection PubMed
description As two cultivated widely allotetraploid cotton species, although Gossypium hirsutum and Gossypium barbadense evolved from the same ancestor, they differ in fiber quality; the molecular mechanism of that difference should be deeply studied. Here, we performed RNA-seq of fiber samples from four G. hirsutum and three G. barbadense cultivars to compare their gene expression patterns on multiple dimensions. We found that 15.90–37.96% of differentially expressed genes showed biased expression toward the A or D subgenome. In particular, interspecific biased expression was exhibited by a total of 330 and 486 gene pairs at 10 days post-anthesis (DPA) and 20 DPA, respectively. Moreover, 6791 genes demonstrated temporal differences in expression, including 346 genes predominantly expressed at 10 DPA in G. hirsutum (TM-1) but postponed to 20 DPA in G. barbadense (Hai7124), and 367 genes predominantly expressed at 20 DPA in TM-1 but postponed to 25 DPA in Hai7124. These postponed genes mainly participated in carbohydrate metabolism, lipid metabolism, plant hormone signal transduction, and starch and sucrose metabolism. In addition, most of the co-expression network and hub genes involved in fiber development showed asymmetric expression between TM-1 and Hai7124, like three hub genes detected at 10 DPA in TM-1 but not until 25 DPA in Hai7124. Our study provides new insights into interspecific expression bias and postponed expression of genes associated with fiber quality, which are mainly tied to asymmetric hub gene network. This work will facilitate further research aimed at understanding the mechanisms underlying cotton fiber improvement.
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spelling pubmed-87337332022-01-07 Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species Mei, Huan Qi, Bowen Han, Zegang Zhao, Ting Guo, Menglan Han, Jin Zhang, Juncheng Guan, Xueying Hu, Yan Zhang, Tianzhen Fang, Lei Front Plant Sci Plant Science As two cultivated widely allotetraploid cotton species, although Gossypium hirsutum and Gossypium barbadense evolved from the same ancestor, they differ in fiber quality; the molecular mechanism of that difference should be deeply studied. Here, we performed RNA-seq of fiber samples from four G. hirsutum and three G. barbadense cultivars to compare their gene expression patterns on multiple dimensions. We found that 15.90–37.96% of differentially expressed genes showed biased expression toward the A or D subgenome. In particular, interspecific biased expression was exhibited by a total of 330 and 486 gene pairs at 10 days post-anthesis (DPA) and 20 DPA, respectively. Moreover, 6791 genes demonstrated temporal differences in expression, including 346 genes predominantly expressed at 10 DPA in G. hirsutum (TM-1) but postponed to 20 DPA in G. barbadense (Hai7124), and 367 genes predominantly expressed at 20 DPA in TM-1 but postponed to 25 DPA in Hai7124. These postponed genes mainly participated in carbohydrate metabolism, lipid metabolism, plant hormone signal transduction, and starch and sucrose metabolism. In addition, most of the co-expression network and hub genes involved in fiber development showed asymmetric expression between TM-1 and Hai7124, like three hub genes detected at 10 DPA in TM-1 but not until 25 DPA in Hai7124. Our study provides new insights into interspecific expression bias and postponed expression of genes associated with fiber quality, which are mainly tied to asymmetric hub gene network. This work will facilitate further research aimed at understanding the mechanisms underlying cotton fiber improvement. Frontiers Media S.A. 2021-12-23 /pmc/articles/PMC8733733/ /pubmed/35003198 http://dx.doi.org/10.3389/fpls.2021.819679 Text en Copyright © 2021 Mei, Qi, Han, Zhao, Guo, Han, Zhang, Guan, Hu, Zhang and Fang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mei, Huan
Qi, Bowen
Han, Zegang
Zhao, Ting
Guo, Menglan
Han, Jin
Zhang, Juncheng
Guan, Xueying
Hu, Yan
Zhang, Tianzhen
Fang, Lei
Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title_full Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title_fullStr Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title_full_unstemmed Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title_short Subgenome Bias and Temporal Postponement of Gene Expression Contributes to the Distinctions of Fiber Quality in Gossypium Species
title_sort subgenome bias and temporal postponement of gene expression contributes to the distinctions of fiber quality in gossypium species
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733733/
https://www.ncbi.nlm.nih.gov/pubmed/35003198
http://dx.doi.org/10.3389/fpls.2021.819679
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