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Using crystallography tools to improve vaccine formulations
This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer’s patches in the sma...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733884/ https://www.ncbi.nlm.nih.gov/pubmed/35059205 http://dx.doi.org/10.1107/S205225252101071X |
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author | Fantini, Márcia Carvalho de Abreu Oliveira, Cristiano Luis Pinto Lopes, José Luiz de Souza Martins, Tereza da Silva Akamatsu, Milena Apetito Trezena, Aryene Góes Franco, Milene Tino-De- Botosso, Viviane Fongaro Sant’Anna, Osvaldo Augusto Brazil Esteves Kardjilov, Nikolay Rasmussen, Martin Kjaerulf Bordallo, Heloísa Nunes |
author_facet | Fantini, Márcia Carvalho de Abreu Oliveira, Cristiano Luis Pinto Lopes, José Luiz de Souza Martins, Tereza da Silva Akamatsu, Milena Apetito Trezena, Aryene Góes Franco, Milene Tino-De- Botosso, Viviane Fongaro Sant’Anna, Osvaldo Augusto Brazil Esteves Kardjilov, Nikolay Rasmussen, Martin Kjaerulf Bordallo, Heloísa Nunes |
author_sort | Fantini, Márcia Carvalho de Abreu |
collection | PubMed |
description | This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer’s patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses. |
format | Online Article Text |
id | pubmed-8733884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-87338842022-01-19 Using crystallography tools to improve vaccine formulations Fantini, Márcia Carvalho de Abreu Oliveira, Cristiano Luis Pinto Lopes, José Luiz de Souza Martins, Tereza da Silva Akamatsu, Milena Apetito Trezena, Aryene Góes Franco, Milene Tino-De- Botosso, Viviane Fongaro Sant’Anna, Osvaldo Augusto Brazil Esteves Kardjilov, Nikolay Rasmussen, Martin Kjaerulf Bordallo, Heloísa Nunes IUCrJ Topical Reviews This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer’s patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses. International Union of Crystallography 2021-11-25 /pmc/articles/PMC8733884/ /pubmed/35059205 http://dx.doi.org/10.1107/S205225252101071X Text en © Márcia Carvalho de Abreu Fantini et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Topical Reviews Fantini, Márcia Carvalho de Abreu Oliveira, Cristiano Luis Pinto Lopes, José Luiz de Souza Martins, Tereza da Silva Akamatsu, Milena Apetito Trezena, Aryene Góes Franco, Milene Tino-De- Botosso, Viviane Fongaro Sant’Anna, Osvaldo Augusto Brazil Esteves Kardjilov, Nikolay Rasmussen, Martin Kjaerulf Bordallo, Heloísa Nunes Using crystallography tools to improve vaccine formulations |
title | Using crystallography tools to improve vaccine formulations |
title_full | Using crystallography tools to improve vaccine formulations |
title_fullStr | Using crystallography tools to improve vaccine formulations |
title_full_unstemmed | Using crystallography tools to improve vaccine formulations |
title_short | Using crystallography tools to improve vaccine formulations |
title_sort | using crystallography tools to improve vaccine formulations |
topic | Topical Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733884/ https://www.ncbi.nlm.nih.gov/pubmed/35059205 http://dx.doi.org/10.1107/S205225252101071X |
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