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Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy

Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold stand...

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Autores principales: Estève, Emmanuel, Buob, David, Jamme, Frédéric, Jouanneau, Chantal, Kascakova, Slavka, Haymann, Jean-Philippe, Letavernier, Emmanuel, Galmiche, Louise, Ronco, Pierre, Daudon, Michel, Bazin, Dominique, Réfrégiers, Matthieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733991/
https://www.ncbi.nlm.nih.gov/pubmed/34985438
http://dx.doi.org/10.1107/S1600577521011371
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author Estève, Emmanuel
Buob, David
Jamme, Frédéric
Jouanneau, Chantal
Kascakova, Slavka
Haymann, Jean-Philippe
Letavernier, Emmanuel
Galmiche, Louise
Ronco, Pierre
Daudon, Michel
Bazin, Dominique
Réfrégiers, Matthieu
author_facet Estève, Emmanuel
Buob, David
Jamme, Frédéric
Jouanneau, Chantal
Kascakova, Slavka
Haymann, Jean-Philippe
Letavernier, Emmanuel
Galmiche, Louise
Ronco, Pierre
Daudon, Michel
Bazin, Dominique
Réfrégiers, Matthieu
author_sort Estève, Emmanuel
collection PubMed
description Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono-, di- and tri-hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non-aggregated oxalate accumulation in tubular cells. A nine-points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio.
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spelling pubmed-87339912022-01-19 Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy Estève, Emmanuel Buob, David Jamme, Frédéric Jouanneau, Chantal Kascakova, Slavka Haymann, Jean-Philippe Letavernier, Emmanuel Galmiche, Louise Ronco, Pierre Daudon, Michel Bazin, Dominique Réfrégiers, Matthieu J Synchrotron Radiat Research Papers Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono-, di- and tri-hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non-aggregated oxalate accumulation in tubular cells. A nine-points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio. International Union of Crystallography 2022-01-01 /pmc/articles/PMC8733991/ /pubmed/34985438 http://dx.doi.org/10.1107/S1600577521011371 Text en © Emmanuel Esteve et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Estève, Emmanuel
Buob, David
Jamme, Frédéric
Jouanneau, Chantal
Kascakova, Slavka
Haymann, Jean-Philippe
Letavernier, Emmanuel
Galmiche, Louise
Ronco, Pierre
Daudon, Michel
Bazin, Dominique
Réfrégiers, Matthieu
Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title_full Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title_fullStr Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title_full_unstemmed Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title_short Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
title_sort detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733991/
https://www.ncbi.nlm.nih.gov/pubmed/34985438
http://dx.doi.org/10.1107/S1600577521011371
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