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Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images

Virtual histology is increasingly utilized to reconstruct the cell mechanisms underlying dental morphology for fragile fossils when physical thin sections are not permitted. Yet, the comparability of data derived from virtual and physical thin sections is rarely tested. Here, the results from archae...

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Autores principales: Nava, Alessia, Mahoney, Patrick, Bondioli, Luca, Coppa, Alfredo, Cristiani, Emanuela, Fattore, Luciano, McFarlane, Gina, Dreossi, Diego, Mancini, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733994/
https://www.ncbi.nlm.nih.gov/pubmed/34985442
http://dx.doi.org/10.1107/S160057752101208X
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author Nava, Alessia
Mahoney, Patrick
Bondioli, Luca
Coppa, Alfredo
Cristiani, Emanuela
Fattore, Luciano
McFarlane, Gina
Dreossi, Diego
Mancini, Lucia
author_facet Nava, Alessia
Mahoney, Patrick
Bondioli, Luca
Coppa, Alfredo
Cristiani, Emanuela
Fattore, Luciano
McFarlane, Gina
Dreossi, Diego
Mancini, Lucia
author_sort Nava, Alessia
collection PubMed
description Virtual histology is increasingly utilized to reconstruct the cell mechanisms underlying dental morphology for fragile fossils when physical thin sections are not permitted. Yet, the comparability of data derived from virtual and physical thin sections is rarely tested. Here, the results from archaeological human deciduous incisor physical sections are compared with virtual ones obtained by phase-contrast synchrotron radiation computed microtomography (SRµCT) of intact specimens using a multi-scale approach. Moreover, virtual prenatal daily enamel secretion rates are compared with those calculated from physical thin sections of the same tooth class from the same archaeological skeletal series. Results showed overall good visibility of the enamel microstructures in the virtual sections which are comparable to that of physical ones. The highest spatial resolution SRµCT setting (effective pixel size = 0.9 µm) produced daily secretion rates that matched those calculated from physical sections. Rates obtained using the lowest spatial resolution setup (effective pixel size = 2.0 µm) were higher than those obtained from physical sections. The results demonstrate that virtual histology can be applied to the investigated samples to obtain reliable and quantitative measurements of prenatal daily enamel secretion rates.
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spelling pubmed-87339942022-01-19 Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images Nava, Alessia Mahoney, Patrick Bondioli, Luca Coppa, Alfredo Cristiani, Emanuela Fattore, Luciano McFarlane, Gina Dreossi, Diego Mancini, Lucia J Synchrotron Radiat Short Communications Virtual histology is increasingly utilized to reconstruct the cell mechanisms underlying dental morphology for fragile fossils when physical thin sections are not permitted. Yet, the comparability of data derived from virtual and physical thin sections is rarely tested. Here, the results from archaeological human deciduous incisor physical sections are compared with virtual ones obtained by phase-contrast synchrotron radiation computed microtomography (SRµCT) of intact specimens using a multi-scale approach. Moreover, virtual prenatal daily enamel secretion rates are compared with those calculated from physical thin sections of the same tooth class from the same archaeological skeletal series. Results showed overall good visibility of the enamel microstructures in the virtual sections which are comparable to that of physical ones. The highest spatial resolution SRµCT setting (effective pixel size = 0.9 µm) produced daily secretion rates that matched those calculated from physical sections. Rates obtained using the lowest spatial resolution setup (effective pixel size = 2.0 µm) were higher than those obtained from physical sections. The results demonstrate that virtual histology can be applied to the investigated samples to obtain reliable and quantitative measurements of prenatal daily enamel secretion rates. International Union of Crystallography 2022-01-01 /pmc/articles/PMC8733994/ /pubmed/34985442 http://dx.doi.org/10.1107/S160057752101208X Text en © Alessia Nava et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Short Communications
Nava, Alessia
Mahoney, Patrick
Bondioli, Luca
Coppa, Alfredo
Cristiani, Emanuela
Fattore, Luciano
McFarlane, Gina
Dreossi, Diego
Mancini, Lucia
Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title_full Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title_fullStr Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title_full_unstemmed Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title_short Virtual histology of archaeological human deciduous prenatal enamel through synchrotron X-ray computed microtomography images
title_sort virtual histology of archaeological human deciduous prenatal enamel through synchrotron x-ray computed microtomography images
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733994/
https://www.ncbi.nlm.nih.gov/pubmed/34985442
http://dx.doi.org/10.1107/S160057752101208X
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