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Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host

Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there i...

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Autores principales: Oliveira, Fernando, Lima, Tânia, Correia, Alexandra, Silva, Ana Margarida, Soares, Cristina, Morais, Simone, Weißelberg, Samira, Vilanova, Manuel, Rohde, Holger, Cerca, Nuno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738164/
https://www.ncbi.nlm.nih.gov/pubmed/35004774
http://dx.doi.org/10.3389/fmed.2021.799227
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author Oliveira, Fernando
Lima, Tânia
Correia, Alexandra
Silva, Ana Margarida
Soares, Cristina
Morais, Simone
Weißelberg, Samira
Vilanova, Manuel
Rohde, Holger
Cerca, Nuno
author_facet Oliveira, Fernando
Lima, Tânia
Correia, Alexandra
Silva, Ana Margarida
Soares, Cristina
Morais, Simone
Weißelberg, Samira
Vilanova, Manuel
Rohde, Holger
Cerca, Nuno
author_sort Oliveira, Fernando
collection PubMed
description Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host.
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spelling pubmed-87381642022-01-08 Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host Oliveira, Fernando Lima, Tânia Correia, Alexandra Silva, Ana Margarida Soares, Cristina Morais, Simone Weißelberg, Samira Vilanova, Manuel Rohde, Holger Cerca, Nuno Front Med (Lausanne) Medicine Iron acquisition through siderophores, a class of small, potent iron-chelating organic molecules, is a widely spread strategy among pathogens to survive in the iron-restricted environment found in the host. Although these molecules have been implicated in the pathogenesis of several species, there is currently no comprehensive study addressing siderophore production in Staphylococcus epidermidis. Staphylococcus epidermidis is an innocuous skin commensal bacterium. The species, though, has emerged as a leading cause of implant-associated infections, significantly supported by an inherent ability to form biofilms. The process of adaptation from skin niche environments to the hostile conditions during invasion is yet not fully understood. Herein, we addressed the possible role of siderophore production in S. epidermidis virulence. We first identified and deleted a siderophore homolog locus, sfaABCD, and provided evidence for its involvement in iron acquisition. Our findings further suggested the involvement of siderophores in the protection against oxidative stress-induced damage and demonstrated the in vivo relevance of a siderophore-mediated iron acquisition during S. epidermidis infections. Conclusively, this study addressed, for the first time in this species, the underlying mechanisms of siderophore production, highlighting the importance of a siderophore-mediated iron acquisition under host relevant conditions and, most importantly, its contribution to survival within the host. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8738164/ /pubmed/35004774 http://dx.doi.org/10.3389/fmed.2021.799227 Text en Copyright © 2021 Oliveira, Lima, Correia, Silva, Soares, Morais, Weißelberg, Vilanova, Rohde and Cerca. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Oliveira, Fernando
Lima, Tânia
Correia, Alexandra
Silva, Ana Margarida
Soares, Cristina
Morais, Simone
Weißelberg, Samira
Vilanova, Manuel
Rohde, Holger
Cerca, Nuno
Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title_full Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title_fullStr Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title_full_unstemmed Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title_short Siderophore-Mediated Iron Acquisition Plays a Critical Role in Biofilm Formation and Survival of Staphylococcus epidermidis Within the Host
title_sort siderophore-mediated iron acquisition plays a critical role in biofilm formation and survival of staphylococcus epidermidis within the host
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738164/
https://www.ncbi.nlm.nih.gov/pubmed/35004774
http://dx.doi.org/10.3389/fmed.2021.799227
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