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Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma
Around 30–50% of classical Hodgkin lymphoma (cHL) cases in immunocompetent individuals from industrialized countries are associated with the B-lymphotropic Epstein-Barr virus (EBV). Although natural killer (NK) cells exhibit anti-viral and anti-tumoral functions, virtually nothing is known about qua...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738354/ https://www.ncbi.nlm.nih.gov/pubmed/33993319 http://dx.doi.org/10.1007/s00262-021-02956-x |
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author | Pánisová, Elena Lünemann, Anna Bürgler, Simone Kotur, Monika Lazarovici, Julien Danu, Alina Kaulfuss, Meike Mietz, Juliane Chijioke, Obinna Münz, Christian Busson, Pierre Berger, Christoph Ghez, David Azzi, Tarik |
author_facet | Pánisová, Elena Lünemann, Anna Bürgler, Simone Kotur, Monika Lazarovici, Julien Danu, Alina Kaulfuss, Meike Mietz, Juliane Chijioke, Obinna Münz, Christian Busson, Pierre Berger, Christoph Ghez, David Azzi, Tarik |
author_sort | Pánisová, Elena |
collection | PubMed |
description | Around 30–50% of classical Hodgkin lymphoma (cHL) cases in immunocompetent individuals from industrialized countries are associated with the B-lymphotropic Epstein-Barr virus (EBV). Although natural killer (NK) cells exhibit anti-viral and anti-tumoral functions, virtually nothing is known about quantitative and qualitative differences in NK cells in patients with EBV+ cHL vs. EBV- cHL. Here, we prospectively investigated 36 cHL patients without known immune suppression or overt immunodeficiency at diagnosis. All 10 EBV+ cHL patients and 25 out 26 EBV- cHL were seropositive for EBV antibodies, and EBV+ cHL patients presented with higher plasma EBV DNA levels compared to EBV- cHL patients. We show that the CD56(dim) CD16(+) NK cell subset was decreased in frequency in EBV+ cHL patients compared to EBV- cHL patients. This quantitative deficiency translates into an impaired CD56(dim) NK cell mediated degranulation toward rituximab-coated HLA class 1 negative lymphoblastoid cells in EBV+ compared to EBV- cHL patients. We finally observed a trend to a decrease in the rituximab-associated degranulation and ADCC of in vitro expanded NK cells of EBV+ cHL compared to healthy controls. Our findings may impact on the design of adjunctive treatment targeting antibody-dependent cellular cytotoxicity in EBV+ cHL. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00262-021-02956-x. |
format | Online Article Text |
id | pubmed-8738354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-87383542022-01-20 Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma Pánisová, Elena Lünemann, Anna Bürgler, Simone Kotur, Monika Lazarovici, Julien Danu, Alina Kaulfuss, Meike Mietz, Juliane Chijioke, Obinna Münz, Christian Busson, Pierre Berger, Christoph Ghez, David Azzi, Tarik Cancer Immunol Immunother Original Article Around 30–50% of classical Hodgkin lymphoma (cHL) cases in immunocompetent individuals from industrialized countries are associated with the B-lymphotropic Epstein-Barr virus (EBV). Although natural killer (NK) cells exhibit anti-viral and anti-tumoral functions, virtually nothing is known about quantitative and qualitative differences in NK cells in patients with EBV+ cHL vs. EBV- cHL. Here, we prospectively investigated 36 cHL patients without known immune suppression or overt immunodeficiency at diagnosis. All 10 EBV+ cHL patients and 25 out 26 EBV- cHL were seropositive for EBV antibodies, and EBV+ cHL patients presented with higher plasma EBV DNA levels compared to EBV- cHL patients. We show that the CD56(dim) CD16(+) NK cell subset was decreased in frequency in EBV+ cHL patients compared to EBV- cHL patients. This quantitative deficiency translates into an impaired CD56(dim) NK cell mediated degranulation toward rituximab-coated HLA class 1 negative lymphoblastoid cells in EBV+ compared to EBV- cHL patients. We finally observed a trend to a decrease in the rituximab-associated degranulation and ADCC of in vitro expanded NK cells of EBV+ cHL compared to healthy controls. Our findings may impact on the design of adjunctive treatment targeting antibody-dependent cellular cytotoxicity in EBV+ cHL. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00262-021-02956-x. Springer Berlin Heidelberg 2021-05-15 2022 /pmc/articles/PMC8738354/ /pubmed/33993319 http://dx.doi.org/10.1007/s00262-021-02956-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Pánisová, Elena Lünemann, Anna Bürgler, Simone Kotur, Monika Lazarovici, Julien Danu, Alina Kaulfuss, Meike Mietz, Juliane Chijioke, Obinna Münz, Christian Busson, Pierre Berger, Christoph Ghez, David Azzi, Tarik Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title | Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title_full | Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title_fullStr | Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title_full_unstemmed | Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title_short | Reduced frequency of cytotoxic CD56(dim) CD16(+) NK cells leads to impaired antibody-dependent degranulation in EBV-positive classical Hodgkin lymphoma |
title_sort | reduced frequency of cytotoxic cd56(dim) cd16(+) nk cells leads to impaired antibody-dependent degranulation in ebv-positive classical hodgkin lymphoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738354/ https://www.ncbi.nlm.nih.gov/pubmed/33993319 http://dx.doi.org/10.1007/s00262-021-02956-x |
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