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The effect of shock duration on trauma-induced coagulopathy in a murine model
BACKGROUND: Trauma-induced coagulopathy (TIC) is a life-threatening condition associated with high morbidity and mortality. TIC can present with different coagulation defects. In this study, the aim was to determine the effect of shock duration on TIC characteristics. We hypothesized that longer dur...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738789/ https://www.ncbi.nlm.nih.gov/pubmed/34993669 http://dx.doi.org/10.1186/s40635-021-00428-1 |
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author | Sloos, Pieter H. Maas, M. Adrie W. Hollmann, Markus W. Juffermans, Nicole P. Kleinveld, Derek J. B. |
author_facet | Sloos, Pieter H. Maas, M. Adrie W. Hollmann, Markus W. Juffermans, Nicole P. Kleinveld, Derek J. B. |
author_sort | Sloos, Pieter H. |
collection | PubMed |
description | BACKGROUND: Trauma-induced coagulopathy (TIC) is a life-threatening condition associated with high morbidity and mortality. TIC can present with different coagulation defects. In this study, the aim was to determine the effect of shock duration on TIC characteristics. We hypothesized that longer duration of shock leads to a more hypocoagulable rotational thromboelastometry (ROTEM) profile compared to a shorter duration of shock. METHODS: Male B57BL/6J(c) mice (n = 5–10 per group) were sedated and mechanically ventilated. Trauma was induced by bilateral lower limb fractures and crush injuries to the liver and small intestine. Shock was induced by blood withdrawals until a mean arterial pressure of 25–30 mmHg was achieved. Groups reflected trauma and shock for 30 min (TS30) and trauma and shock for 90 min (TS90). Control groups included ventilation only (V90) and trauma only (T90). RESULTS: Mice in the TS90 group had significantly increased base deficit compared to the V90 group. Mortality was 10% in the TS30 group and 30% in the TS90 group. ROTEM profile was more hypocoagulable, as shown by significantly lower maximum clot firmness (MCF) in the TS30 group (43.5 [37.5–46.8] mm) compared to the TS90 group (52.0 [47.0–53.0] mm, p = 0.04). ROTEM clotting time and parameters of clot build-up did not significantly differ between groups. CONCLUSIONS: TIC characteristics change with shock duration. Contrary to the hypothesis, a shorter duration of shock was associated with decreased maximum clotting amplitudes compared to a longer duration of shock. The effect of shock duration on TIC should be further assessed in trauma patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40635-021-00428-1. |
format | Online Article Text |
id | pubmed-8738789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-87387892022-01-20 The effect of shock duration on trauma-induced coagulopathy in a murine model Sloos, Pieter H. Maas, M. Adrie W. Hollmann, Markus W. Juffermans, Nicole P. Kleinveld, Derek J. B. Intensive Care Med Exp Research Articles BACKGROUND: Trauma-induced coagulopathy (TIC) is a life-threatening condition associated with high morbidity and mortality. TIC can present with different coagulation defects. In this study, the aim was to determine the effect of shock duration on TIC characteristics. We hypothesized that longer duration of shock leads to a more hypocoagulable rotational thromboelastometry (ROTEM) profile compared to a shorter duration of shock. METHODS: Male B57BL/6J(c) mice (n = 5–10 per group) were sedated and mechanically ventilated. Trauma was induced by bilateral lower limb fractures and crush injuries to the liver and small intestine. Shock was induced by blood withdrawals until a mean arterial pressure of 25–30 mmHg was achieved. Groups reflected trauma and shock for 30 min (TS30) and trauma and shock for 90 min (TS90). Control groups included ventilation only (V90) and trauma only (T90). RESULTS: Mice in the TS90 group had significantly increased base deficit compared to the V90 group. Mortality was 10% in the TS30 group and 30% in the TS90 group. ROTEM profile was more hypocoagulable, as shown by significantly lower maximum clot firmness (MCF) in the TS30 group (43.5 [37.5–46.8] mm) compared to the TS90 group (52.0 [47.0–53.0] mm, p = 0.04). ROTEM clotting time and parameters of clot build-up did not significantly differ between groups. CONCLUSIONS: TIC characteristics change with shock duration. Contrary to the hypothesis, a shorter duration of shock was associated with decreased maximum clotting amplitudes compared to a longer duration of shock. The effect of shock duration on TIC should be further assessed in trauma patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40635-021-00428-1. Springer International Publishing 2022-01-07 /pmc/articles/PMC8738789/ /pubmed/34993669 http://dx.doi.org/10.1186/s40635-021-00428-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Sloos, Pieter H. Maas, M. Adrie W. Hollmann, Markus W. Juffermans, Nicole P. Kleinveld, Derek J. B. The effect of shock duration on trauma-induced coagulopathy in a murine model |
title | The effect of shock duration on trauma-induced coagulopathy in a murine model |
title_full | The effect of shock duration on trauma-induced coagulopathy in a murine model |
title_fullStr | The effect of shock duration on trauma-induced coagulopathy in a murine model |
title_full_unstemmed | The effect of shock duration on trauma-induced coagulopathy in a murine model |
title_short | The effect of shock duration on trauma-induced coagulopathy in a murine model |
title_sort | effect of shock duration on trauma-induced coagulopathy in a murine model |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8738789/ https://www.ncbi.nlm.nih.gov/pubmed/34993669 http://dx.doi.org/10.1186/s40635-021-00428-1 |
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