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Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake

PURPOSE: Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogen...

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Autores principales: Coto, Laura, Sousa, Carolina, Cebolla, Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739026/
https://www.ncbi.nlm.nih.gov/pubmed/34993643
http://dx.doi.org/10.1007/s00394-021-02765-z
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author Coto, Laura
Sousa, Carolina
Cebolla, Angel
author_facet Coto, Laura
Sousa, Carolina
Cebolla, Angel
author_sort Coto, Laura
collection PubMed
description PURPOSE: Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogeneously controlled dietary conditions, and the sensitivity of fecal GIP tests after low amounts of punctual gluten ingestions. METHODS: Participants (n = 20) followed the same gluten-free menu for 12 days in which two separated doses of gluten (50 mg and 2 g) were ingested and all the depositions were collected. GIP from stool samples were analyzed by ELISA and lateral flow immunoassay (LFIA) tests. RESULTS: Most participants had detectable GIP after 50 mg and 2 g gluten ingestions using ELISA test (72.2% and 95%, respectively), whereas the LFIA test showed less sensitivity (22.2% and 80%, respectively). GIP were detected at higher either frequency or concentration in the range of 12–36 h after 50 mg intake, and 12–84 h after 2 g consumption. Considering this period, diagnostic sensitivity of GIP detection after a single 50 mg ingestion may be significatively increased analyzing three stool samples per individual. High variability among participants was found in the time and amount of GIP excretion; however, some individuals showed common patterns for both gluten intakes. CONCLUSION: Sporadic gluten exposure detection may require several fecal samples to achieve level of sensitivity above 90%. Interindividual variability in the dynamic of GIP excretion may suggest patterns of gluten metabolism.
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spelling pubmed-87390262022-01-07 Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake Coto, Laura Sousa, Carolina Cebolla, Angel Eur J Nutr Original Contribution PURPOSE: Determination of Gluten Immunogenic Peptides (GIP) in feces is a direct tool for gluten exposure detection. The sensitivity of GIP detection methods for cases of unintentional low gluten intakes is unknown. We studied the interindividual variability in the kinetic of excretion under homogeneously controlled dietary conditions, and the sensitivity of fecal GIP tests after low amounts of punctual gluten ingestions. METHODS: Participants (n = 20) followed the same gluten-free menu for 12 days in which two separated doses of gluten (50 mg and 2 g) were ingested and all the depositions were collected. GIP from stool samples were analyzed by ELISA and lateral flow immunoassay (LFIA) tests. RESULTS: Most participants had detectable GIP after 50 mg and 2 g gluten ingestions using ELISA test (72.2% and 95%, respectively), whereas the LFIA test showed less sensitivity (22.2% and 80%, respectively). GIP were detected at higher either frequency or concentration in the range of 12–36 h after 50 mg intake, and 12–84 h after 2 g consumption. Considering this period, diagnostic sensitivity of GIP detection after a single 50 mg ingestion may be significatively increased analyzing three stool samples per individual. High variability among participants was found in the time and amount of GIP excretion; however, some individuals showed common patterns for both gluten intakes. CONCLUSION: Sporadic gluten exposure detection may require several fecal samples to achieve level of sensitivity above 90%. Interindividual variability in the dynamic of GIP excretion may suggest patterns of gluten metabolism. Springer Berlin Heidelberg 2022-01-07 2022 /pmc/articles/PMC8739026/ /pubmed/34993643 http://dx.doi.org/10.1007/s00394-021-02765-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Contribution
Coto, Laura
Sousa, Carolina
Cebolla, Angel
Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_full Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_fullStr Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_full_unstemmed Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_short Individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
title_sort individual variability in patterns and dynamics of fecal gluten immunogenic peptides excretion after low gluten intake
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739026/
https://www.ncbi.nlm.nih.gov/pubmed/34993643
http://dx.doi.org/10.1007/s00394-021-02765-z
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