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φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestio...

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Autores principales: Filik, Karolina, Szermer-Olearnik, Bożena, Niedziółka-Jönson, Joanna, Roźniecka, Ewa, Ciekot, Jarosław, Pyra, Anna, Matyjaszczyk, Irwin, Skurnik, Mikael, Brzozowska, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739404/
https://www.ncbi.nlm.nih.gov/pubmed/34989907
http://dx.doi.org/10.1186/s13568-021-01341-2
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author Filik, Karolina
Szermer-Olearnik, Bożena
Niedziółka-Jönson, Joanna
Roźniecka, Ewa
Ciekot, Jarosław
Pyra, Anna
Matyjaszczyk, Irwin
Skurnik, Mikael
Brzozowska, Ewa
author_facet Filik, Karolina
Szermer-Olearnik, Bożena
Niedziółka-Jönson, Joanna
Roźniecka, Ewa
Ciekot, Jarosław
Pyra, Anna
Matyjaszczyk, Irwin
Skurnik, Mikael
Brzozowska, Ewa
author_sort Filik, Karolina
collection PubMed
description Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selective growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, especially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01341-2.
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spelling pubmed-87394042022-01-20 φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3 Filik, Karolina Szermer-Olearnik, Bożena Niedziółka-Jönson, Joanna Roźniecka, Ewa Ciekot, Jarosław Pyra, Anna Matyjaszczyk, Irwin Skurnik, Mikael Brzozowska, Ewa AMB Express Original Article Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selective growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, especially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01341-2. Springer Berlin Heidelberg 2022-01-06 /pmc/articles/PMC8739404/ /pubmed/34989907 http://dx.doi.org/10.1186/s13568-021-01341-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Filik, Karolina
Szermer-Olearnik, Bożena
Niedziółka-Jönson, Joanna
Roźniecka, Ewa
Ciekot, Jarosław
Pyra, Anna
Matyjaszczyk, Irwin
Skurnik, Mikael
Brzozowska, Ewa
φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title_full φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title_fullStr φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title_full_unstemmed φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title_short φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
title_sort φyeo3-12 phage tail fiber gp17 as a promising high specific tool for recognition of yersinia enterocolitica pathogenic serotype o:3
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739404/
https://www.ncbi.nlm.nih.gov/pubmed/34989907
http://dx.doi.org/10.1186/s13568-021-01341-2
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