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CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis

Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study...

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Autores principales: Yang, Jian, Tian, Shuaiwei, Wang, Baocheng, Wang, Jiajia, Cao, Liangliang, Wang, Qinhua, Xie, Wanqun, Liang, Zhuangzhuang, Zhao, Heng, Zhao, Yang, Liao, Keman, Ma, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739489/
https://www.ncbi.nlm.nih.gov/pubmed/35004326
http://dx.doi.org/10.3389/fonc.2021.801776
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author Yang, Jian
Tian, Shuaiwei
Wang, Baocheng
Wang, Jiajia
Cao, Liangliang
Wang, Qinhua
Xie, Wanqun
Liang, Zhuangzhuang
Zhao, Heng
Zhao, Yang
Liao, Keman
Ma, Jie
author_facet Yang, Jian
Tian, Shuaiwei
Wang, Baocheng
Wang, Jiajia
Cao, Liangliang
Wang, Qinhua
Xie, Wanqun
Liang, Zhuangzhuang
Zhao, Heng
Zhao, Yang
Liao, Keman
Ma, Jie
author_sort Yang, Jian
collection PubMed
description Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study aims to investigate the roles and underlying mechanism of circPIK3C2A in regulating proliferation and invasion of glioblastoma. qRT-PCR assays showed that the expression level of circPIK3C2A was aberrantly higher in glioblastoma cell lines, in comparison with that in normal glia cells. The ectopic expression of circPIK3C2A promoted the proliferation, invasion and clonal formation of glioblastoma cells, while circPIK3C2A loss-of-function exerted exactly the opposite biological effects on the cells. The construction of subcutaneous xenograft tumor model in nude mice indicated that circPIK3C2A loss-of-function effectively diminished tumor load in vivo and prolonged the survival time of tumor-bearing animals. Luciferase reporter assay confirmed the interaction among circPIK3C2A/miR-877-5p and FOXM1. CircPIK3C2A function as competitive endogenous RNA via sponging miR-877-5p through certain binding sites, thereby modulating the expression of FOXM1. Our results collectively indicate that circPIK3C2A functions as ceRNA by mediating miR-877-5p/FOXM1 axis, providing a novel perspective of applying CircPIK3C2A in the clinical intervention of glioblastoma in the future.
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spelling pubmed-87394892022-01-08 CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis Yang, Jian Tian, Shuaiwei Wang, Baocheng Wang, Jiajia Cao, Liangliang Wang, Qinhua Xie, Wanqun Liang, Zhuangzhuang Zhao, Heng Zhao, Yang Liao, Keman Ma, Jie Front Oncol Oncology Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study aims to investigate the roles and underlying mechanism of circPIK3C2A in regulating proliferation and invasion of glioblastoma. qRT-PCR assays showed that the expression level of circPIK3C2A was aberrantly higher in glioblastoma cell lines, in comparison with that in normal glia cells. The ectopic expression of circPIK3C2A promoted the proliferation, invasion and clonal formation of glioblastoma cells, while circPIK3C2A loss-of-function exerted exactly the opposite biological effects on the cells. The construction of subcutaneous xenograft tumor model in nude mice indicated that circPIK3C2A loss-of-function effectively diminished tumor load in vivo and prolonged the survival time of tumor-bearing animals. Luciferase reporter assay confirmed the interaction among circPIK3C2A/miR-877-5p and FOXM1. CircPIK3C2A function as competitive endogenous RNA via sponging miR-877-5p through certain binding sites, thereby modulating the expression of FOXM1. Our results collectively indicate that circPIK3C2A functions as ceRNA by mediating miR-877-5p/FOXM1 axis, providing a novel perspective of applying CircPIK3C2A in the clinical intervention of glioblastoma in the future. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8739489/ /pubmed/35004326 http://dx.doi.org/10.3389/fonc.2021.801776 Text en Copyright © 2021 Yang, Tian, Wang, Wang, Cao, Wang, Xie, Liang, Zhao, Zhao, Liao and Ma https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Yang, Jian
Tian, Shuaiwei
Wang, Baocheng
Wang, Jiajia
Cao, Liangliang
Wang, Qinhua
Xie, Wanqun
Liang, Zhuangzhuang
Zhao, Heng
Zhao, Yang
Liao, Keman
Ma, Jie
CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title_full CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title_fullStr CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title_full_unstemmed CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title_short CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis
title_sort circpik3c2a facilitates the progression of glioblastoma via targeting mir-877-5p/foxm1 axis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739489/
https://www.ncbi.nlm.nih.gov/pubmed/35004326
http://dx.doi.org/10.3389/fonc.2021.801776
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