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Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients

CONTEXT: Measurements of thyroglobulin (Tg) and Tg antibodies are crucial in the follow-up of treated differentiated thyroid cancer (DTC) patients. Interassay differences may significantly impact follow-up. OBJECTIVE: The aim of this multicenter study was to explore the impact of Tg and Tg antibody...

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Autores principales: Schoonen, Lise, Neele, Marjolein, van Toor, Hans, van Kinschot, Caroline M J, van Noord, Charlotte, Visser, W Edward, Groen, Joost, Boesten, Lianne S M, Lentjes, Eef G W M, van den Berg, Sjoerd A A, Kos, Snjezana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739647/
https://www.ncbi.nlm.nih.gov/pubmed/35024538
http://dx.doi.org/10.1210/jendso/bvab166
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author Schoonen, Lise
Neele, Marjolein
van Toor, Hans
van Kinschot, Caroline M J
van Noord, Charlotte
Visser, W Edward
Groen, Joost
Boesten, Lianne S M
Lentjes, Eef G W M
van den Berg, Sjoerd A A
Kos, Snjezana
author_facet Schoonen, Lise
Neele, Marjolein
van Toor, Hans
van Kinschot, Caroline M J
van Noord, Charlotte
Visser, W Edward
Groen, Joost
Boesten, Lianne S M
Lentjes, Eef G W M
van den Berg, Sjoerd A A
Kos, Snjezana
author_sort Schoonen, Lise
collection PubMed
description CONTEXT: Measurements of thyroglobulin (Tg) and Tg antibodies are crucial in the follow-up of treated differentiated thyroid cancer (DTC) patients. Interassay differences may significantly impact follow-up. OBJECTIVE: The aim of this multicenter study was to explore the impact of Tg and Tg antibody assay performance on the differential classification of DTC patients, as described in national and international guidelines. DESIGN: Four commonly used Tg and Tg antibody assays were technically compared to reflect possible effects on patients with DTC follow-up. Storage stability at different storage temperatures was also investigated for LIAISON® and Kryptor assays, as this is an underexposed topic in current literature. RESULTS: B.R.A.H.M.S. assays yield approximately 50% lower Tg values over the whole range compared to the DiaSorin and Roche assays investigated. These differences between assays may result in potential misclassification in up to 7% of patients if fixed cutoffs (eg, 1 ng/mL) are applied. Poor correlation was also observed between the Tg antibody assays when the method-specific upper limits of normal are used as cutoffs. Storage of Tg and Tg antibodies was possible for 3 to 4 weeks at −20°C and −80°C. Calibration of the assays, however, was found to be crucial for stable results over time. CONCLUSIONS: Technical aspects of Tg and Tg antibody assays, including interassay differences, calibration and standardization, and cutoff values, may have a significant clinical impact on the follow-up of DTC patients.
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spelling pubmed-87396472022-01-11 Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients Schoonen, Lise Neele, Marjolein van Toor, Hans van Kinschot, Caroline M J van Noord, Charlotte Visser, W Edward Groen, Joost Boesten, Lianne S M Lentjes, Eef G W M van den Berg, Sjoerd A A Kos, Snjezana J Endocr Soc Clinical Research Article CONTEXT: Measurements of thyroglobulin (Tg) and Tg antibodies are crucial in the follow-up of treated differentiated thyroid cancer (DTC) patients. Interassay differences may significantly impact follow-up. OBJECTIVE: The aim of this multicenter study was to explore the impact of Tg and Tg antibody assay performance on the differential classification of DTC patients, as described in national and international guidelines. DESIGN: Four commonly used Tg and Tg antibody assays were technically compared to reflect possible effects on patients with DTC follow-up. Storage stability at different storage temperatures was also investigated for LIAISON® and Kryptor assays, as this is an underexposed topic in current literature. RESULTS: B.R.A.H.M.S. assays yield approximately 50% lower Tg values over the whole range compared to the DiaSorin and Roche assays investigated. These differences between assays may result in potential misclassification in up to 7% of patients if fixed cutoffs (eg, 1 ng/mL) are applied. Poor correlation was also observed between the Tg antibody assays when the method-specific upper limits of normal are used as cutoffs. Storage of Tg and Tg antibodies was possible for 3 to 4 weeks at −20°C and −80°C. Calibration of the assays, however, was found to be crucial for stable results over time. CONCLUSIONS: Technical aspects of Tg and Tg antibody assays, including interassay differences, calibration and standardization, and cutoff values, may have a significant clinical impact on the follow-up of DTC patients. Oxford University Press 2021-11-10 /pmc/articles/PMC8739647/ /pubmed/35024538 http://dx.doi.org/10.1210/jendso/bvab166 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Clinical Research Article
Schoonen, Lise
Neele, Marjolein
van Toor, Hans
van Kinschot, Caroline M J
van Noord, Charlotte
Visser, W Edward
Groen, Joost
Boesten, Lianne S M
Lentjes, Eef G W M
van den Berg, Sjoerd A A
Kos, Snjezana
Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title_full Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title_fullStr Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title_full_unstemmed Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title_short Impact of Thyroglobulin and Thyroglobulin Antibody Assay Performance on the Differential Classification of DTC Patients
title_sort impact of thyroglobulin and thyroglobulin antibody assay performance on the differential classification of dtc patients
topic Clinical Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739647/
https://www.ncbi.nlm.nih.gov/pubmed/35024538
http://dx.doi.org/10.1210/jendso/bvab166
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