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Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma
BACKGROUND: The advance of new treatment strategies for more effective management of oral cancer requires identification of novel biological targets. Therefore, the purpose of this study is to identify novel biomarkers associated with oral tumorigenesis and prognostic signature by comparing gene exp...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739923/ https://www.ncbi.nlm.nih.gov/pubmed/35003322 http://dx.doi.org/10.1155/2021/5802110 |
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author | Xie, Pengfeng Wu, Shichao Guo, Lijuan Ren, Jun Cai, Kaizhi Zhou, Mingyue Liu, Weiwei Yang, Sen |
author_facet | Xie, Pengfeng Wu, Shichao Guo, Lijuan Ren, Jun Cai, Kaizhi Zhou, Mingyue Liu, Weiwei Yang, Sen |
author_sort | Xie, Pengfeng |
collection | PubMed |
description | BACKGROUND: The advance of new treatment strategies for more effective management of oral cancer requires identification of novel biological targets. Therefore, the purpose of this study is to identify novel biomarkers associated with oral tumorigenesis and prognostic signature by comparing gene expression profile of oral squamous cell carcinomas (OSCCs). METHODS: Four datasets including GSE25099, GSE30784, GSE37991, and GSE41613 were collected from Gene Expression Omnibus (GEO) database. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, Cox model analysis, identification of key genes, and Kaplan-Meier analysis were also performed. The xCell was utilized to analyze the infiltration levels of immune cells. RESULTS: A total of 235 differentially expressed genes (DEGs) were found to be dysregulated in OSCC. These genes were mainly enriched in ECM receptor interaction and focal adhesion. Cox regression analysis identified 10 genes considered as key genes. Kaplan-Meier analysis showed that low expression of SERPINE1 (also known as PAI-1), high expression of CD1C, and C-X3-C motif chemokine receptor 1 (CX3CR1) were associated with well prognostic status in OSCC patients. In addition, we constructed a 3-immune-cell signature (myeloid dendritic cell, T cell CD4(+) central memory, and common myeloid progenitor) that may be used to predict the survival status of OSCC patients. CONCLUSION: Three key genes and 3-immune-cell signature were potential biomarkers for the prognosis of OSCC, and they may serve as potential targets for the treatment of OSCC patients. |
format | Online Article Text |
id | pubmed-8739923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-87399232022-01-08 Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma Xie, Pengfeng Wu, Shichao Guo, Lijuan Ren, Jun Cai, Kaizhi Zhou, Mingyue Liu, Weiwei Yang, Sen Comput Math Methods Med Research Article BACKGROUND: The advance of new treatment strategies for more effective management of oral cancer requires identification of novel biological targets. Therefore, the purpose of this study is to identify novel biomarkers associated with oral tumorigenesis and prognostic signature by comparing gene expression profile of oral squamous cell carcinomas (OSCCs). METHODS: Four datasets including GSE25099, GSE30784, GSE37991, and GSE41613 were collected from Gene Expression Omnibus (GEO) database. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, Cox model analysis, identification of key genes, and Kaplan-Meier analysis were also performed. The xCell was utilized to analyze the infiltration levels of immune cells. RESULTS: A total of 235 differentially expressed genes (DEGs) were found to be dysregulated in OSCC. These genes were mainly enriched in ECM receptor interaction and focal adhesion. Cox regression analysis identified 10 genes considered as key genes. Kaplan-Meier analysis showed that low expression of SERPINE1 (also known as PAI-1), high expression of CD1C, and C-X3-C motif chemokine receptor 1 (CX3CR1) were associated with well prognostic status in OSCC patients. In addition, we constructed a 3-immune-cell signature (myeloid dendritic cell, T cell CD4(+) central memory, and common myeloid progenitor) that may be used to predict the survival status of OSCC patients. CONCLUSION: Three key genes and 3-immune-cell signature were potential biomarkers for the prognosis of OSCC, and they may serve as potential targets for the treatment of OSCC patients. Hindawi 2021-12-30 /pmc/articles/PMC8739923/ /pubmed/35003322 http://dx.doi.org/10.1155/2021/5802110 Text en Copyright © 2021 Pengfeng Xie et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xie, Pengfeng Wu, Shichao Guo, Lijuan Ren, Jun Cai, Kaizhi Zhou, Mingyue Liu, Weiwei Yang, Sen Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title | Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title_full | Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title_fullStr | Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title_full_unstemmed | Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title_short | Identification of Candidate Target Genes and Immune Cells in Oral Squamous Cell Carcinoma |
title_sort | identification of candidate target genes and immune cells in oral squamous cell carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739923/ https://www.ncbi.nlm.nih.gov/pubmed/35003322 http://dx.doi.org/10.1155/2021/5802110 |
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