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Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor

BACKGROUND: Histone deacetylases (HDACs) play an important role in the regulation of gene expression, which is indispensable in plant growth, development, and responses to environmental stresses. In Arabidopsis and rice, the molecular functions of HDACs have been well-described. However, systematic...

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Autores principales: Du, Qiaoli, Fang, Yuanpeng, Jiang, Junmei, Chen, Meiqing, Fu, Xiaodong, Yang, Zaifu, Luo, Liting, Wu, Qijiao, Yang, Qian, Wang, Lujie, Qu, Zhiguang, Li, Xiangyang, Xie, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739980/
https://www.ncbi.nlm.nih.gov/pubmed/34991465
http://dx.doi.org/10.1186/s12864-021-08229-2
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author Du, Qiaoli
Fang, Yuanpeng
Jiang, Junmei
Chen, Meiqing
Fu, Xiaodong
Yang, Zaifu
Luo, Liting
Wu, Qijiao
Yang, Qian
Wang, Lujie
Qu, Zhiguang
Li, Xiangyang
Xie, Xin
author_facet Du, Qiaoli
Fang, Yuanpeng
Jiang, Junmei
Chen, Meiqing
Fu, Xiaodong
Yang, Zaifu
Luo, Liting
Wu, Qijiao
Yang, Qian
Wang, Lujie
Qu, Zhiguang
Li, Xiangyang
Xie, Xin
author_sort Du, Qiaoli
collection PubMed
description BACKGROUND: Histone deacetylases (HDACs) play an important role in the regulation of gene expression, which is indispensable in plant growth, development, and responses to environmental stresses. In Arabidopsis and rice, the molecular functions of HDACs have been well-described. However, systematic analysis of the HDAC gene family and gene expression in response to biotic and abiotic stresses has not been reported for sorghum. RESULTS: We conducted a systematic analysis of the sorghum HDAC gene family and identified 19 SbHDACs mainly distributed on eight chromosomes. Phylogenetic tree analysis of SbHDACs showed that the gene family was divided into three subfamilies: RPD3/HDA1, SIR2, and HD2. Tissue-specific expression results showed that SbHDACs displayed different expression patterns in different tissues, indicating that these genes may perform different functions in growth and development. The expression pattern of SbHDACs under different stresses (high and low temperature, drought, osmotic and salt) and pathogen-associated molecular model (PAMPs) elf18, chitin, and flg22) indicated that SbHDAC genes may participate in adversity responses and biological stress defenses. Overexpression of SbHDA1, SbHDA3, SbHDT2 and SbSRT2 in Escherichia coli promoted the growth of recombinant cells under abiotic stress. Interestingly, we also showed that the sorghum acetylation level was enhanced when plants were under cold, heat, drought, osmotic and salt stresses. The findings will help us to understand the HDAC gene family in sorghum, and illuminate the molecular mechanism of the responses to abiotic and biotic stresses. CONCLUSION: We have identified and classified 19 HDAC genes in sorghum. Our data provides insights into the evolution of the HDAC gene family and further support the hypothesis that these genes are important for the plant responses to abiotic and biotic stresses. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08229-2.
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spelling pubmed-87399802022-01-07 Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor Du, Qiaoli Fang, Yuanpeng Jiang, Junmei Chen, Meiqing Fu, Xiaodong Yang, Zaifu Luo, Liting Wu, Qijiao Yang, Qian Wang, Lujie Qu, Zhiguang Li, Xiangyang Xie, Xin BMC Genomics Research BACKGROUND: Histone deacetylases (HDACs) play an important role in the regulation of gene expression, which is indispensable in plant growth, development, and responses to environmental stresses. In Arabidopsis and rice, the molecular functions of HDACs have been well-described. However, systematic analysis of the HDAC gene family and gene expression in response to biotic and abiotic stresses has not been reported for sorghum. RESULTS: We conducted a systematic analysis of the sorghum HDAC gene family and identified 19 SbHDACs mainly distributed on eight chromosomes. Phylogenetic tree analysis of SbHDACs showed that the gene family was divided into three subfamilies: RPD3/HDA1, SIR2, and HD2. Tissue-specific expression results showed that SbHDACs displayed different expression patterns in different tissues, indicating that these genes may perform different functions in growth and development. The expression pattern of SbHDACs under different stresses (high and low temperature, drought, osmotic and salt) and pathogen-associated molecular model (PAMPs) elf18, chitin, and flg22) indicated that SbHDAC genes may participate in adversity responses and biological stress defenses. Overexpression of SbHDA1, SbHDA3, SbHDT2 and SbSRT2 in Escherichia coli promoted the growth of recombinant cells under abiotic stress. Interestingly, we also showed that the sorghum acetylation level was enhanced when plants were under cold, heat, drought, osmotic and salt stresses. The findings will help us to understand the HDAC gene family in sorghum, and illuminate the molecular mechanism of the responses to abiotic and biotic stresses. CONCLUSION: We have identified and classified 19 HDAC genes in sorghum. Our data provides insights into the evolution of the HDAC gene family and further support the hypothesis that these genes are important for the plant responses to abiotic and biotic stresses. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08229-2. BioMed Central 2022-01-06 /pmc/articles/PMC8739980/ /pubmed/34991465 http://dx.doi.org/10.1186/s12864-021-08229-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Du, Qiaoli
Fang, Yuanpeng
Jiang, Junmei
Chen, Meiqing
Fu, Xiaodong
Yang, Zaifu
Luo, Liting
Wu, Qijiao
Yang, Qian
Wang, Lujie
Qu, Zhiguang
Li, Xiangyang
Xie, Xin
Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title_full Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title_fullStr Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title_full_unstemmed Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title_short Characterization of histone deacetylases and their roles in response to abiotic and PAMPs stresses in Sorghum bicolor
title_sort characterization of histone deacetylases and their roles in response to abiotic and pamps stresses in sorghum bicolor
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739980/
https://www.ncbi.nlm.nih.gov/pubmed/34991465
http://dx.doi.org/10.1186/s12864-021-08229-2
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