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WUSCHEL-related homeobox family genes in rice control lateral root primordium size
The development of a plastic root system is essential for stable crop production under variable environments. Rice plants have two types of lateral roots (LRs): S-type (short and thin) and L-type (long, thick, and capable of further branching). LR types are determined at the primordium stage, with a...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8740593/ https://www.ncbi.nlm.nih.gov/pubmed/34983834 http://dx.doi.org/10.1073/pnas.2101846119 |
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author | Kawai, Tsubasa Shibata, Kyosuke Akahoshi, Ryosuke Nishiuchi, Shunsaku Takahashi, Hirokazu Nakazono, Mikio Kojima, Takaaki Nosaka-Takahashi, Misuzu Sato, Yutaka Toyoda, Atsushi Lucob-Agustin, Nonawin Kano-Nakata, Mana Suralta, Roel R. Niones, Jonathan M. Chen, Yinglong Siddique, Kadambot H. M. Yamauchi, Akira Inukai, Yoshiaki |
author_facet | Kawai, Tsubasa Shibata, Kyosuke Akahoshi, Ryosuke Nishiuchi, Shunsaku Takahashi, Hirokazu Nakazono, Mikio Kojima, Takaaki Nosaka-Takahashi, Misuzu Sato, Yutaka Toyoda, Atsushi Lucob-Agustin, Nonawin Kano-Nakata, Mana Suralta, Roel R. Niones, Jonathan M. Chen, Yinglong Siddique, Kadambot H. M. Yamauchi, Akira Inukai, Yoshiaki |
author_sort | Kawai, Tsubasa |
collection | PubMed |
description | The development of a plastic root system is essential for stable crop production under variable environments. Rice plants have two types of lateral roots (LRs): S-type (short and thin) and L-type (long, thick, and capable of further branching). LR types are determined at the primordium stage, with a larger primordium size in L-types than S-types. Despite the importance of LR types for rice adaptability to variable water conditions, molecular mechanisms underlying the primordium size control of LRs are unknown. Here, we show that two WUSCHEL-related homeobox (WOX) genes have opposing roles in controlling LR primordium (LRP) size in rice. Root tip excision on seminal roots induced L-type LR formation with wider primordia formed from an early developmental stage. QHB/OsWOX5 was isolated as a causative gene of a mutant that is defective in S-type LR formation but produces more L-type LRs than wild-type (WT) plants following root tip excision. A transcriptome analysis revealed that OsWOX10 is highly up-regulated in L-type LRPs. OsWOX10 overexpression in LRPs increased the LR diameter in an expression-dependent manner. Conversely, the mutation in OsWOX10 decreased the L-type LR diameter under mild drought conditions. The qhb mutants had higher OsWOX10 expression than WT after root tip excision. A yeast one-hybrid assay revealed that the transcriptional repressive activity of QHB was lost in qhb mutants. An electrophoresis mobility shift assay revealed that OsWOX10 is a potential target of QHB. These data suggest that QHB represses LR diameter increase, repressing OsWOX10. Our findings could help improve root system plasticity under variable environments. |
format | Online Article Text |
id | pubmed-8740593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-87405932022-01-25 WUSCHEL-related homeobox family genes in rice control lateral root primordium size Kawai, Tsubasa Shibata, Kyosuke Akahoshi, Ryosuke Nishiuchi, Shunsaku Takahashi, Hirokazu Nakazono, Mikio Kojima, Takaaki Nosaka-Takahashi, Misuzu Sato, Yutaka Toyoda, Atsushi Lucob-Agustin, Nonawin Kano-Nakata, Mana Suralta, Roel R. Niones, Jonathan M. Chen, Yinglong Siddique, Kadambot H. M. Yamauchi, Akira Inukai, Yoshiaki Proc Natl Acad Sci U S A Biological Sciences The development of a plastic root system is essential for stable crop production under variable environments. Rice plants have two types of lateral roots (LRs): S-type (short and thin) and L-type (long, thick, and capable of further branching). LR types are determined at the primordium stage, with a larger primordium size in L-types than S-types. Despite the importance of LR types for rice adaptability to variable water conditions, molecular mechanisms underlying the primordium size control of LRs are unknown. Here, we show that two WUSCHEL-related homeobox (WOX) genes have opposing roles in controlling LR primordium (LRP) size in rice. Root tip excision on seminal roots induced L-type LR formation with wider primordia formed from an early developmental stage. QHB/OsWOX5 was isolated as a causative gene of a mutant that is defective in S-type LR formation but produces more L-type LRs than wild-type (WT) plants following root tip excision. A transcriptome analysis revealed that OsWOX10 is highly up-regulated in L-type LRPs. OsWOX10 overexpression in LRPs increased the LR diameter in an expression-dependent manner. Conversely, the mutation in OsWOX10 decreased the L-type LR diameter under mild drought conditions. The qhb mutants had higher OsWOX10 expression than WT after root tip excision. A yeast one-hybrid assay revealed that the transcriptional repressive activity of QHB was lost in qhb mutants. An electrophoresis mobility shift assay revealed that OsWOX10 is a potential target of QHB. These data suggest that QHB represses LR diameter increase, repressing OsWOX10. Our findings could help improve root system plasticity under variable environments. National Academy of Sciences 2021-12-30 2022-01-04 /pmc/articles/PMC8740593/ /pubmed/34983834 http://dx.doi.org/10.1073/pnas.2101846119 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Kawai, Tsubasa Shibata, Kyosuke Akahoshi, Ryosuke Nishiuchi, Shunsaku Takahashi, Hirokazu Nakazono, Mikio Kojima, Takaaki Nosaka-Takahashi, Misuzu Sato, Yutaka Toyoda, Atsushi Lucob-Agustin, Nonawin Kano-Nakata, Mana Suralta, Roel R. Niones, Jonathan M. Chen, Yinglong Siddique, Kadambot H. M. Yamauchi, Akira Inukai, Yoshiaki WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title | WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title_full | WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title_fullStr | WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title_full_unstemmed | WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title_short | WUSCHEL-related homeobox family genes in rice control lateral root primordium size |
title_sort | wuschel-related homeobox family genes in rice control lateral root primordium size |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8740593/ https://www.ncbi.nlm.nih.gov/pubmed/34983834 http://dx.doi.org/10.1073/pnas.2101846119 |
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