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Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy

BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p...

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Autores principales: Okamura, Takuro, Okada, Hiroshi, Hashimoto, Yoshitaka, Majima, Saori, Senmaru, Takafumi, Nakanishi, Naoko, Asano, Mai, Yamazaki, Masahiro, Hamaguchi, Masahide, Fukui, Michiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741024/
https://www.ncbi.nlm.nih.gov/pubmed/35002969
http://dx.doi.org/10.3389/fendo.2021.791363
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author Okamura, Takuro
Okada, Hiroshi
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Hamaguchi, Masahide
Fukui, Michiaki
author_facet Okamura, Takuro
Okada, Hiroshi
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Hamaguchi, Masahide
Fukui, Michiaki
author_sort Okamura, Takuro
collection PubMed
description BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p in the muscle, the effect of let-7e-5p overexpression or knockdown on the expression of myosin heavy chain, glucose uptake, and mitochondrial function was investigated in C2C12 myotube cells. Moreover, we examined serum let-7e-5p levels among male subjects with type 2 diabetes. RESULTS: We found that the expression of the miRNA, lethal (let)-7e-5p was significantly lower in ORX mice than that in Sham mice (p = 0.027); however, let-7e-5p expression in androgen-treated ORX mice was higher (p = 0.047). Suppression of let-7e-5p significantly upregulated the expression of myosin heavy chain, glucose uptake, and mitochondrial function. Real-time PCR revealed a possible regulation involving let-7e-5p and Igf2bp2 mRNA and protein in C2C12 cells. The serum let-7e-5p levels were significantly lower, which might be in compensation, in subjects with decreased muscle mass compared to subjects without decreased muscle mass. Let-7e-5p downregulates the expression of Igf2bp2 in myotube cells and inhibits the growth of the myosin heavy chain. CONCLUSIONS: Based on our study, serum level of let-7e-5p may be used as a potential diagnostic marker for muscle atrophy.
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spelling pubmed-87410242022-01-08 Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy Okamura, Takuro Okada, Hiroshi Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Hamaguchi, Masahide Fukui, Michiaki Front Endocrinol (Lausanne) Endocrinology BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p in the muscle, the effect of let-7e-5p overexpression or knockdown on the expression of myosin heavy chain, glucose uptake, and mitochondrial function was investigated in C2C12 myotube cells. Moreover, we examined serum let-7e-5p levels among male subjects with type 2 diabetes. RESULTS: We found that the expression of the miRNA, lethal (let)-7e-5p was significantly lower in ORX mice than that in Sham mice (p = 0.027); however, let-7e-5p expression in androgen-treated ORX mice was higher (p = 0.047). Suppression of let-7e-5p significantly upregulated the expression of myosin heavy chain, glucose uptake, and mitochondrial function. Real-time PCR revealed a possible regulation involving let-7e-5p and Igf2bp2 mRNA and protein in C2C12 cells. The serum let-7e-5p levels were significantly lower, which might be in compensation, in subjects with decreased muscle mass compared to subjects without decreased muscle mass. Let-7e-5p downregulates the expression of Igf2bp2 in myotube cells and inhibits the growth of the myosin heavy chain. CONCLUSIONS: Based on our study, serum level of let-7e-5p may be used as a potential diagnostic marker for muscle atrophy. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8741024/ /pubmed/35002969 http://dx.doi.org/10.3389/fendo.2021.791363 Text en Copyright © 2021 Okamura, Okada, Hashimoto, Majima, Senmaru, Nakanishi, Asano, Yamazaki, Hamaguchi and Fukui https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Okamura, Takuro
Okada, Hiroshi
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Hamaguchi, Masahide
Fukui, Michiaki
Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title_full Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title_fullStr Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title_full_unstemmed Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title_short Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
title_sort let-7e-5p regulates igf2bp2, and induces muscle atrophy
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741024/
https://www.ncbi.nlm.nih.gov/pubmed/35002969
http://dx.doi.org/10.3389/fendo.2021.791363
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