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Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy
BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741024/ https://www.ncbi.nlm.nih.gov/pubmed/35002969 http://dx.doi.org/10.3389/fendo.2021.791363 |
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author | Okamura, Takuro Okada, Hiroshi Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Hamaguchi, Masahide Fukui, Michiaki |
author_facet | Okamura, Takuro Okada, Hiroshi Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Hamaguchi, Masahide Fukui, Michiaki |
author_sort | Okamura, Takuro |
collection | PubMed |
description | BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p in the muscle, the effect of let-7e-5p overexpression or knockdown on the expression of myosin heavy chain, glucose uptake, and mitochondrial function was investigated in C2C12 myotube cells. Moreover, we examined serum let-7e-5p levels among male subjects with type 2 diabetes. RESULTS: We found that the expression of the miRNA, lethal (let)-7e-5p was significantly lower in ORX mice than that in Sham mice (p = 0.027); however, let-7e-5p expression in androgen-treated ORX mice was higher (p = 0.047). Suppression of let-7e-5p significantly upregulated the expression of myosin heavy chain, glucose uptake, and mitochondrial function. Real-time PCR revealed a possible regulation involving let-7e-5p and Igf2bp2 mRNA and protein in C2C12 cells. The serum let-7e-5p levels were significantly lower, which might be in compensation, in subjects with decreased muscle mass compared to subjects without decreased muscle mass. Let-7e-5p downregulates the expression of Igf2bp2 in myotube cells and inhibits the growth of the myosin heavy chain. CONCLUSIONS: Based on our study, serum level of let-7e-5p may be used as a potential diagnostic marker for muscle atrophy. |
format | Online Article Text |
id | pubmed-8741024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87410242022-01-08 Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy Okamura, Takuro Okada, Hiroshi Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Hamaguchi, Masahide Fukui, Michiaki Front Endocrinol (Lausanne) Endocrinology BACKGROUND AND AIMS: To understand the role of microRNAs in muscle atrophy caused by androgen-depletion, we performed microarray analysis of microRNA expression in the skeletal muscles of Sham, orchiectomized (ORX), and androgen-treated ORX mice. METHODS: To clarify role and mechanisms of let-7e-5p in the muscle, the effect of let-7e-5p overexpression or knockdown on the expression of myosin heavy chain, glucose uptake, and mitochondrial function was investigated in C2C12 myotube cells. Moreover, we examined serum let-7e-5p levels among male subjects with type 2 diabetes. RESULTS: We found that the expression of the miRNA, lethal (let)-7e-5p was significantly lower in ORX mice than that in Sham mice (p = 0.027); however, let-7e-5p expression in androgen-treated ORX mice was higher (p = 0.047). Suppression of let-7e-5p significantly upregulated the expression of myosin heavy chain, glucose uptake, and mitochondrial function. Real-time PCR revealed a possible regulation involving let-7e-5p and Igf2bp2 mRNA and protein in C2C12 cells. The serum let-7e-5p levels were significantly lower, which might be in compensation, in subjects with decreased muscle mass compared to subjects without decreased muscle mass. Let-7e-5p downregulates the expression of Igf2bp2 in myotube cells and inhibits the growth of the myosin heavy chain. CONCLUSIONS: Based on our study, serum level of let-7e-5p may be used as a potential diagnostic marker for muscle atrophy. Frontiers Media S.A. 2021-12-24 /pmc/articles/PMC8741024/ /pubmed/35002969 http://dx.doi.org/10.3389/fendo.2021.791363 Text en Copyright © 2021 Okamura, Okada, Hashimoto, Majima, Senmaru, Nakanishi, Asano, Yamazaki, Hamaguchi and Fukui https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Okamura, Takuro Okada, Hiroshi Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Hamaguchi, Masahide Fukui, Michiaki Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title | Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title_full | Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title_fullStr | Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title_full_unstemmed | Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title_short | Let-7e-5p Regulates IGF2BP2, and Induces Muscle Atrophy |
title_sort | let-7e-5p regulates igf2bp2, and induces muscle atrophy |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741024/ https://www.ncbi.nlm.nih.gov/pubmed/35002969 http://dx.doi.org/10.3389/fendo.2021.791363 |
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