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MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5

BACKGROUND: Although studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. We aimed to investigate the roles of microRNA-603 in cutaneous melanoma cells. METHODS: First, microRNA-603 expression in cuta...

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Autores principales: Dong, Xianghua, Wang, Ying, Qu, Yan, Liu, Junru, Feng, Xien, Xu, Xuechao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741382/
https://www.ncbi.nlm.nih.gov/pubmed/35003317
http://dx.doi.org/10.1155/2021/1888501
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author Dong, Xianghua
Wang, Ying
Qu, Yan
Liu, Junru
Feng, Xien
Xu, Xuechao
author_facet Dong, Xianghua
Wang, Ying
Qu, Yan
Liu, Junru
Feng, Xien
Xu, Xuechao
author_sort Dong, Xianghua
collection PubMed
description BACKGROUND: Although studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. We aimed to investigate the roles of microRNA-603 in cutaneous melanoma cells. METHODS: First, microRNA-603 expression in cutaneous melanoma cell lines was detected by qRT-PCR. The mRNA and protein expression levels of TBX5 in cutaneous melanoma cell lines were tested by qRT-PCR and western blot, respectively. In addition, the interaction between microRNA-603 and TBX5 was determined by dual-luciferase reporter gene assay, and their impacts on the growth of cutaneous melanoma cells were detected by cellular function experiments such as MTT, colony formation, and Transwell assays. RESULTS: The expression level of microRNA-603 in human cutaneous melanoma cells was relatively upregulated. Overexpressing microRNA-603 could promote progression of cutaneous melanoma cells, while silencing microRNA-603 expression could suppress the malignant progression of cutaneous melanoma. In addition, TBX5 was lowly expressed in cutaneous melanoma cells. As confirmed by dual-luciferase assay, microRNA-603 could specifically bind to 3′UTR of TBX5 and regulate TBX5. The results of the rescue experiment demonstrated that inhibiting microRNA-603 expression could suppress the proliferation, migration, and invasion of cutaneous melanoma cells, but its suppressive effect could be restored by TBX5. CONCLUSION: MicroRNA-603 could regulate the expression of TBX5, thus promoting the malignant progression of cutaneous melanoma cells.
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spelling pubmed-87413822022-01-08 MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5 Dong, Xianghua Wang, Ying Qu, Yan Liu, Junru Feng, Xien Xu, Xuechao Comput Math Methods Med Research Article BACKGROUND: Although studies manifested that microRNA-603 plays a vital role in many cancers, the modulatory mechanism of microRNA-603 in cutaneous melanoma remains unknown. We aimed to investigate the roles of microRNA-603 in cutaneous melanoma cells. METHODS: First, microRNA-603 expression in cutaneous melanoma cell lines was detected by qRT-PCR. The mRNA and protein expression levels of TBX5 in cutaneous melanoma cell lines were tested by qRT-PCR and western blot, respectively. In addition, the interaction between microRNA-603 and TBX5 was determined by dual-luciferase reporter gene assay, and their impacts on the growth of cutaneous melanoma cells were detected by cellular function experiments such as MTT, colony formation, and Transwell assays. RESULTS: The expression level of microRNA-603 in human cutaneous melanoma cells was relatively upregulated. Overexpressing microRNA-603 could promote progression of cutaneous melanoma cells, while silencing microRNA-603 expression could suppress the malignant progression of cutaneous melanoma. In addition, TBX5 was lowly expressed in cutaneous melanoma cells. As confirmed by dual-luciferase assay, microRNA-603 could specifically bind to 3′UTR of TBX5 and regulate TBX5. The results of the rescue experiment demonstrated that inhibiting microRNA-603 expression could suppress the proliferation, migration, and invasion of cutaneous melanoma cells, but its suppressive effect could be restored by TBX5. CONCLUSION: MicroRNA-603 could regulate the expression of TBX5, thus promoting the malignant progression of cutaneous melanoma cells. Hindawi 2021-12-31 /pmc/articles/PMC8741382/ /pubmed/35003317 http://dx.doi.org/10.1155/2021/1888501 Text en Copyright © 2021 Xianghua Dong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dong, Xianghua
Wang, Ying
Qu, Yan
Liu, Junru
Feng, Xien
Xu, Xuechao
MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title_full MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title_fullStr MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title_full_unstemmed MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title_short MicroRNA-603 Promotes Progression of Cutaneous Melanoma by Regulating TBX5
title_sort microrna-603 promotes progression of cutaneous melanoma by regulating tbx5
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741382/
https://www.ncbi.nlm.nih.gov/pubmed/35003317
http://dx.doi.org/10.1155/2021/1888501
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