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Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma

Lighting conditions may affect the development of retinal degenerative diseases such as macular degeneration. In this study, to determine whether the lighting environment affects the progression of degeneration of retinal ganglion cells (RGCs), we examined glutamate/aspartate transporter (GLAST) het...

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Autores principales: Ohashi, Tsutomu, Namekata, Kazuhiko, Guo, Xiaoli, Kimura, Atsuko, Harada, Chikako, Harada, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741416/
https://www.ncbi.nlm.nih.gov/pubmed/35028438
http://dx.doi.org/10.1016/j.bbrep.2021.101197
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author Ohashi, Tsutomu
Namekata, Kazuhiko
Guo, Xiaoli
Kimura, Atsuko
Harada, Chikako
Harada, Takayuki
author_facet Ohashi, Tsutomu
Namekata, Kazuhiko
Guo, Xiaoli
Kimura, Atsuko
Harada, Chikako
Harada, Takayuki
author_sort Ohashi, Tsutomu
collection PubMed
description Lighting conditions may affect the development of retinal degenerative diseases such as macular degeneration. In this study, to determine whether the lighting environment affects the progression of degeneration of retinal ganglion cells (RGCs), we examined glutamate/aspartate transporter (GLAST) heterozygous (GLAST(+/-)) mice, a mouse model of normal tension glaucoma. GLAST(+/-) mice were reared under a 12-h light-dark cycle (Light/Dark) or complete darkness (Dark/Dark) condition after birth. The total RGC number in the Dark/Dark group was significantly decreased compared with the Light/Dark group at 3 weeks old, while the number of osteopontin-positive αRGCs were similar in both groups. At 6 and 12 weeks old, the total RGC number were not significantly different in both conditions. In addition, the retinal function examined by multifocal electroretinogram were similar at 12 weeks old. These results suggest that lighting conditions may regulate the progression of RGC degeneration in some types of glaucoma.
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spelling pubmed-87414162022-01-12 Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma Ohashi, Tsutomu Namekata, Kazuhiko Guo, Xiaoli Kimura, Atsuko Harada, Chikako Harada, Takayuki Biochem Biophys Rep Research Article Lighting conditions may affect the development of retinal degenerative diseases such as macular degeneration. In this study, to determine whether the lighting environment affects the progression of degeneration of retinal ganglion cells (RGCs), we examined glutamate/aspartate transporter (GLAST) heterozygous (GLAST(+/-)) mice, a mouse model of normal tension glaucoma. GLAST(+/-) mice were reared under a 12-h light-dark cycle (Light/Dark) or complete darkness (Dark/Dark) condition after birth. The total RGC number in the Dark/Dark group was significantly decreased compared with the Light/Dark group at 3 weeks old, while the number of osteopontin-positive αRGCs were similar in both groups. At 6 and 12 weeks old, the total RGC number were not significantly different in both conditions. In addition, the retinal function examined by multifocal electroretinogram were similar at 12 weeks old. These results suggest that lighting conditions may regulate the progression of RGC degeneration in some types of glaucoma. Elsevier 2022-01-05 /pmc/articles/PMC8741416/ /pubmed/35028438 http://dx.doi.org/10.1016/j.bbrep.2021.101197 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Ohashi, Tsutomu
Namekata, Kazuhiko
Guo, Xiaoli
Kimura, Atsuko
Harada, Chikako
Harada, Takayuki
Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title_full Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title_fullStr Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title_full_unstemmed Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title_short Effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
title_sort effects of lighting environment on the degeneration of retinal ganglion cells in glutamate/aspartate transporter deficient mice, a mouse model of normal tension glaucoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741416/
https://www.ncbi.nlm.nih.gov/pubmed/35028438
http://dx.doi.org/10.1016/j.bbrep.2021.101197
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