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Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections

Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNPV infecti...

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Autores principales: Tsai, Chih-Hsuan, Chuang, Yi-Chi, Lu, Yun-Heng, Lin, Chia-Yang, Tang, Cheng-Kang, Wei, Sung-Chan, Wu, Yueh-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741431/
https://www.ncbi.nlm.nih.gov/pubmed/35028533
http://dx.doi.org/10.1016/j.isci.2021.103648
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author Tsai, Chih-Hsuan
Chuang, Yi-Chi
Lu, Yun-Heng
Lin, Chia-Yang
Tang, Cheng-Kang
Wei, Sung-Chan
Wu, Yueh-Lung
author_facet Tsai, Chih-Hsuan
Chuang, Yi-Chi
Lu, Yun-Heng
Lin, Chia-Yang
Tang, Cheng-Kang
Wei, Sung-Chan
Wu, Yueh-Lung
author_sort Tsai, Chih-Hsuan
collection PubMed
description Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNPV infecting Spodoptera litura), we found that stored carbohydrates, including hemolymph trehalose and fat body glycogen, are rapidly transformed into glucose; enzymes involved in glycolysis and the TCA cycle are upregulated and produce more ATP; adenosine signaling that regulates glycolytic activity is also increased. Subsequently, phagocytosis in cellular immunity and the expression of genes involved in humoral immunity increase significantly. Moreover, inhibiting glycolysis and the expression of gloverins in nonpermissive hosts increased baculovirus infectivity, indicating that the stimulated energy production is designed to support the immune response against infection. Our study highlights that alteration of the host's carbohydrate metabolism is an important factor determining the host specificity of baculoviruses, in addition to viral factors.
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spelling pubmed-87414312022-01-12 Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections Tsai, Chih-Hsuan Chuang, Yi-Chi Lu, Yun-Heng Lin, Chia-Yang Tang, Cheng-Kang Wei, Sung-Chan Wu, Yueh-Lung iScience Article Baculoviruses Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV) have highly similar genome sequences but exhibit no overlap in their host range. After baculovirus infects nonpermissive larvae (e.g., AcMNPV infecting B. mori or BmNPV infecting Spodoptera litura), we found that stored carbohydrates, including hemolymph trehalose and fat body glycogen, are rapidly transformed into glucose; enzymes involved in glycolysis and the TCA cycle are upregulated and produce more ATP; adenosine signaling that regulates glycolytic activity is also increased. Subsequently, phagocytosis in cellular immunity and the expression of genes involved in humoral immunity increase significantly. Moreover, inhibiting glycolysis and the expression of gloverins in nonpermissive hosts increased baculovirus infectivity, indicating that the stimulated energy production is designed to support the immune response against infection. Our study highlights that alteration of the host's carbohydrate metabolism is an important factor determining the host specificity of baculoviruses, in addition to viral factors. Elsevier 2021-12-18 /pmc/articles/PMC8741431/ /pubmed/35028533 http://dx.doi.org/10.1016/j.isci.2021.103648 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tsai, Chih-Hsuan
Chuang, Yi-Chi
Lu, Yun-Heng
Lin, Chia-Yang
Tang, Cheng-Kang
Wei, Sung-Chan
Wu, Yueh-Lung
Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title_full Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title_fullStr Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title_full_unstemmed Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title_short Carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
title_sort carbohydrate metabolism is a determinant for the host specificity of baculovirus infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741431/
https://www.ncbi.nlm.nih.gov/pubmed/35028533
http://dx.doi.org/10.1016/j.isci.2021.103648
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