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Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey

Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-derived ant...

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Autores principales: Dewi, Grace, Manjankattil, Shijinaraj, Peichel, Claire, Jia, Shiliang, Nair, Divek, Vickers, Zata, Johnson, Timothy J., Cardona, Carol, Noll, Sally, Kollanoor Johny, Anup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741611/
https://www.ncbi.nlm.nih.gov/pubmed/34991037
http://dx.doi.org/10.1016/j.psj.2021.101581
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author Dewi, Grace
Manjankattil, Shijinaraj
Peichel, Claire
Jia, Shiliang
Nair, Divek
Vickers, Zata
Johnson, Timothy J.
Cardona, Carol
Noll, Sally
Kollanoor Johny, Anup
author_facet Dewi, Grace
Manjankattil, Shijinaraj
Peichel, Claire
Jia, Shiliang
Nair, Divek
Vickers, Zata
Johnson, Timothy J.
Cardona, Carol
Noll, Sally
Kollanoor Johny, Anup
author_sort Dewi, Grace
collection PubMed
description Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-derived antimicrobials (PDAs), lemongrass essential oil (LG), citral (CIT), and trans-cinnamaldehyde (TC), against SH in ground turkey, a comminuted product implicated in several outbreaks, was evaluated in this study. Ground turkey samples inoculated with ∼3.50 log(10) CFU/g of a three-strain SH cocktail were treated with either LG, CIT, or TC at either 0.5, 1, or 2% (vol/wt). Samples were stored at 4°C, and bacterial enumeration was performed on d 0, 1, 3, and 5. Appropriate controls were included alongside all treatments. Fluorescence microscopy was performed to evaluate the direct impact of the PDAs against SH in vitro. Appearance and aroma difference testing of raw patties was also performed for select treatments with trained sensory panelists. Treatment with 2% TC yielded a 2.5 log(10) CFU/g reduction by d 1 and complete reduction by d 5 (P < 0.05). By d 3, 2% CIT and 2% LG resulted in SH reduction of at least 1.7 log(10) CFU/g (P < 0.05). Addition of 1% TC resulted in reduction of at least 1.8 log(10) CFU/g by d 3 (P < 0.05). Participants could distinguish PDA-treated raw patties by aroma. Most participants (7/11) could not distinguish patties treated with 0.5% TC based on appearance. Microscopic images indicate that all PDAs resulted in disruption of the SH membrane. Results of the present study indicate that the three tested PDAs, LG, CIT, and TC are effective against SH in ground turkey, indicating their potential use as interventions to mitigate Salmonella contamination in comminuted turkey products.
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spelling pubmed-87416112022-01-12 Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey Dewi, Grace Manjankattil, Shijinaraj Peichel, Claire Jia, Shiliang Nair, Divek Vickers, Zata Johnson, Timothy J. Cardona, Carol Noll, Sally Kollanoor Johny, Anup Poult Sci MICROBIOLOGY AND FOOD SAFETY Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-derived antimicrobials (PDAs), lemongrass essential oil (LG), citral (CIT), and trans-cinnamaldehyde (TC), against SH in ground turkey, a comminuted product implicated in several outbreaks, was evaluated in this study. Ground turkey samples inoculated with ∼3.50 log(10) CFU/g of a three-strain SH cocktail were treated with either LG, CIT, or TC at either 0.5, 1, or 2% (vol/wt). Samples were stored at 4°C, and bacterial enumeration was performed on d 0, 1, 3, and 5. Appropriate controls were included alongside all treatments. Fluorescence microscopy was performed to evaluate the direct impact of the PDAs against SH in vitro. Appearance and aroma difference testing of raw patties was also performed for select treatments with trained sensory panelists. Treatment with 2% TC yielded a 2.5 log(10) CFU/g reduction by d 1 and complete reduction by d 5 (P < 0.05). By d 3, 2% CIT and 2% LG resulted in SH reduction of at least 1.7 log(10) CFU/g (P < 0.05). Addition of 1% TC resulted in reduction of at least 1.8 log(10) CFU/g by d 3 (P < 0.05). Participants could distinguish PDA-treated raw patties by aroma. Most participants (7/11) could not distinguish patties treated with 0.5% TC based on appearance. Microscopic images indicate that all PDAs resulted in disruption of the SH membrane. Results of the present study indicate that the three tested PDAs, LG, CIT, and TC are effective against SH in ground turkey, indicating their potential use as interventions to mitigate Salmonella contamination in comminuted turkey products. Elsevier 2021-11-07 /pmc/articles/PMC8741611/ /pubmed/34991037 http://dx.doi.org/10.1016/j.psj.2021.101581 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle MICROBIOLOGY AND FOOD SAFETY
Dewi, Grace
Manjankattil, Shijinaraj
Peichel, Claire
Jia, Shiliang
Nair, Divek
Vickers, Zata
Johnson, Timothy J.
Cardona, Carol
Noll, Sally
Kollanoor Johny, Anup
Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title_full Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title_fullStr Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title_full_unstemmed Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title_short Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
title_sort effect of plant-derived antimicrobials against multidrug-resistant salmonella heidelberg in ground turkey
topic MICROBIOLOGY AND FOOD SAFETY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741611/
https://www.ncbi.nlm.nih.gov/pubmed/34991037
http://dx.doi.org/10.1016/j.psj.2021.101581
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