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Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2
Vertically paired electrodes (VPEs) with multiple electrode pairs were developed for the enhancement of capacitive measurements by optimizing the electrode gap and number of electrode pairs. The electrode was fabricated using a conductive polymer layer of PEDOT:PSS instead of Ag and Pt metal electro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741629/ https://www.ncbi.nlm.nih.gov/pubmed/35042131 http://dx.doi.org/10.1016/j.bios.2022.113975 |
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author | Park, Jun-Hee Lee, Ga-Yeon Song, Zhiquan Bong, Ji-Hong Chang, Young Wook Cho, Sungbo Kang, Min-Jung Pyun, Jae-Chul |
author_facet | Park, Jun-Hee Lee, Ga-Yeon Song, Zhiquan Bong, Ji-Hong Chang, Young Wook Cho, Sungbo Kang, Min-Jung Pyun, Jae-Chul |
author_sort | Park, Jun-Hee |
collection | PubMed |
description | Vertically paired electrodes (VPEs) with multiple electrode pairs were developed for the enhancement of capacitive measurements by optimizing the electrode gap and number of electrode pairs. The electrode was fabricated using a conductive polymer layer of PEDOT:PSS instead of Ag and Pt metal electrodes to increase the VPE fabrication yield because the PEDOT:PSS layer could be effectively etched using a reactive dry etching process. In this study, sensitivity enhancement was realized by decreasing the electrode gap and increasing the number of VPE electrode pairs. Such an increase in sensitivity according to the electrode gap and the number of electrode pairs was estimated using a model analyte for an immunoassay. Additionally, a computer simulation was performed using VPEs with different electrode gaps and numbers of VPE electrode pairs. Finally, VPEs with multiple electrode pairs were applied for SARS-CoV-2 nucleoprotein (NP) detection. The capacitive biosensor based on the VPE with immobilized anti-SARS-CoV-2 NP was applied for the specific detection of SARS-CoV-2 in viral cultures. Using viral cultures of SARS-CoV-2, SARS-CoV, MERS-CoV, and CoV-strain 229E, the limit of detection (LOD) was estimated to satisfy the cutoff value (dilution factor of 1/800) for the medical diagnosis of COVID-19, and the assay results from the capacitive biosensor were compared with commercial rapid kit based on a lateral flow immunoassay. |
format | Online Article Text |
id | pubmed-8741629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87416292022-01-10 Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 Park, Jun-Hee Lee, Ga-Yeon Song, Zhiquan Bong, Ji-Hong Chang, Young Wook Cho, Sungbo Kang, Min-Jung Pyun, Jae-Chul Biosens Bioelectron Article Vertically paired electrodes (VPEs) with multiple electrode pairs were developed for the enhancement of capacitive measurements by optimizing the electrode gap and number of electrode pairs. The electrode was fabricated using a conductive polymer layer of PEDOT:PSS instead of Ag and Pt metal electrodes to increase the VPE fabrication yield because the PEDOT:PSS layer could be effectively etched using a reactive dry etching process. In this study, sensitivity enhancement was realized by decreasing the electrode gap and increasing the number of VPE electrode pairs. Such an increase in sensitivity according to the electrode gap and the number of electrode pairs was estimated using a model analyte for an immunoassay. Additionally, a computer simulation was performed using VPEs with different electrode gaps and numbers of VPE electrode pairs. Finally, VPEs with multiple electrode pairs were applied for SARS-CoV-2 nucleoprotein (NP) detection. The capacitive biosensor based on the VPE with immobilized anti-SARS-CoV-2 NP was applied for the specific detection of SARS-CoV-2 in viral cultures. Using viral cultures of SARS-CoV-2, SARS-CoV, MERS-CoV, and CoV-strain 229E, the limit of detection (LOD) was estimated to satisfy the cutoff value (dilution factor of 1/800) for the medical diagnosis of COVID-19, and the assay results from the capacitive biosensor were compared with commercial rapid kit based on a lateral flow immunoassay. Elsevier B.V. 2022-04-15 2022-01-08 /pmc/articles/PMC8741629/ /pubmed/35042131 http://dx.doi.org/10.1016/j.bios.2022.113975 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Park, Jun-Hee Lee, Ga-Yeon Song, Zhiquan Bong, Ji-Hong Chang, Young Wook Cho, Sungbo Kang, Min-Jung Pyun, Jae-Chul Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title | Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title_full | Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title_fullStr | Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title_full_unstemmed | Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title_short | Capacitive biosensor based on vertically paired electrodes for the detection of SARS-CoV-2 |
title_sort | capacitive biosensor based on vertically paired electrodes for the detection of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741629/ https://www.ncbi.nlm.nih.gov/pubmed/35042131 http://dx.doi.org/10.1016/j.bios.2022.113975 |
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