Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods

Assessing genuine extracellular vesicle (EV) uptake is crucial for understanding the functional roles of EVs. This study measured the bona fide labelling of EVs utilising two commonly used fluorescent dyes, PKH26 and C5-maleimide-Alexa633. MCF7 EVs tagged with mEmerald-CD81 were isolated from condit...

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Detalles Bibliográficos
Autores principales: Melling, Genevieve E., Conlon, Ross, Pantazi, Paschalia, Dellar, Elizabeth R., Samuel, Priya, Baena-Lopez, Luis Alberto, Simpson, Jeremy C., Carter, David R. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741769/
https://www.ncbi.nlm.nih.gov/pubmed/34997141
http://dx.doi.org/10.1038/s41598-021-04225-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741769/
https://www.ncbi.nlm.nih.gov/pubmed/34997141
http://dx.doi.org/10.1038/s41598-021-04225-4

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