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Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species

Herein, the effect of dietary inclusion of insect (Tenebrio molitor) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss), fed diets at 25%, 50% and 60% ins...

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Autores principales: Mente, Eleni, Bousdras, Thomas, Feidantsis, Konstantinos, Panteli, Nikolas, Mastoraki, Maria, Kormas, Konstantinos Ar., Chatzifotis, Stavros, Piccolo, Giovanni, Gasco, Laura, Gai, Francesco, Martin, Samuel A. M., Antonopoulou, Efthimia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742038/
https://www.ncbi.nlm.nih.gov/pubmed/34996900
http://dx.doi.org/10.1038/s41598-021-03306-8
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author Mente, Eleni
Bousdras, Thomas
Feidantsis, Konstantinos
Panteli, Nikolas
Mastoraki, Maria
Kormas, Konstantinos Ar.
Chatzifotis, Stavros
Piccolo, Giovanni
Gasco, Laura
Gai, Francesco
Martin, Samuel A. M.
Antonopoulou, Efthimia
author_facet Mente, Eleni
Bousdras, Thomas
Feidantsis, Konstantinos
Panteli, Nikolas
Mastoraki, Maria
Kormas, Konstantinos Ar.
Chatzifotis, Stavros
Piccolo, Giovanni
Gasco, Laura
Gai, Francesco
Martin, Samuel A. M.
Antonopoulou, Efthimia
author_sort Mente, Eleni
collection PubMed
description Herein, the effect of dietary inclusion of insect (Tenebrio molitor) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by T. molitor, D. labrax and O. mykiss suppressed apoptosis through induction of hepatic autophagy, while in S. aurata both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.
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spelling pubmed-87420382022-01-11 Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species Mente, Eleni Bousdras, Thomas Feidantsis, Konstantinos Panteli, Nikolas Mastoraki, Maria Kormas, Konstantinos Ar. Chatzifotis, Stavros Piccolo, Giovanni Gasco, Laura Gai, Francesco Martin, Samuel A. M. Antonopoulou, Efthimia Sci Rep Article Herein, the effect of dietary inclusion of insect (Tenebrio molitor) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by T. molitor, D. labrax and O. mykiss suppressed apoptosis through induction of hepatic autophagy, while in S. aurata both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species. Nature Publishing Group UK 2022-01-07 /pmc/articles/PMC8742038/ /pubmed/34996900 http://dx.doi.org/10.1038/s41598-021-03306-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mente, Eleni
Bousdras, Thomas
Feidantsis, Konstantinos
Panteli, Nikolas
Mastoraki, Maria
Kormas, Konstantinos Ar.
Chatzifotis, Stavros
Piccolo, Giovanni
Gasco, Laura
Gai, Francesco
Martin, Samuel A. M.
Antonopoulou, Efthimia
Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title_full Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title_fullStr Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title_full_unstemmed Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title_short Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
title_sort tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742038/
https://www.ncbi.nlm.nih.gov/pubmed/34996900
http://dx.doi.org/10.1038/s41598-021-03306-8
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